Date published: 2025-9-9
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MUS81 Antibody (MTA30 2G10/3) - Western Blotting - Image 363732
MUS81 抗体 (MTA30 2G10/3): sc-53382. A-431 核提取物 (A) 和 Jurkat 全细胞裂解液 (B) 中 MUS81 表达的 Western 印迹分析.
MUS81 抗体 (MTA30 2G10/3): sc-53382. A-431 核提取物 (A) 和 Jurkat 全细胞裂解液 (B) 中 MUS81 表达的 Western 印迹分析.

MUS81 抗体 (MTA30 2G10/3): sc-53382

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寫評論提問

说明书
  • MUS81抗体(MTA30 2G10/3)是小鼠单克隆IgG2a κ, 在26篇文献中引用，规格为200 µg/ml
  • 针对源自human的Mus81
  • 推荐用于 human 来源的MUS81 WB 和 IP检测
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是MUS81 Antibody (MTA30 2G10/3) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与MUS81 Antibody (MTA30 2G10/3) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

DNA修复和检查点反应共同确保基因组的完整性。在遗传毒性辐射或化疗后，细胞周期检查点和DNA修复的协调尤为重要，因为这些治疗过程中会承受异常高的DNA损伤负荷。MUS81编码一种螺旋-发夹-螺旋蛋白，这种蛋白参与酿酒酵母中由紫外线和甲基化诱导的DNA损伤的反应。Mus81对于芽殖酵母和裂殖酵母的复制压力耐受性都很重要。具体而言，Mus81与Eme1结合形成内切核酸酶，这种酶能够在复制叉回归形成Holliday连接之前处理停滞的复制叉。与Mus81相关的内切核酸酶通过仅在相同极性的链上切割连接处，将Holliday连接解析为线性双链体。此外，在暴露于阻断DNA复制的试剂后，Mus81蛋白在细胞中的丰度会增加。Mus81参与将Cds1招募到异常的DNA结构中，其中Cds1调节损伤耐受酶的活性。编码人类MUS81的基因位于染色体11q13上，并编码一个由551个氨基酸组成的蛋白质。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MUS81 抗体 (MTA30 2G10/3) 参考文献:

  1. 人Mus81相关内切酶在体外裂解霍利迪连接。  |  Chen, XB., et al. 2001. Mol Cell. 8: 1117-27. PMID: 11741546
  2. MUS81 内切酶对端粒酶阴性的细胞增殖至关重要。  |  Zeng, S. and Yang, Q. 2009. Cell Cycle. 8: 2157-60. PMID: 19617716
  3. DNA 修复内切酶 Mus81 能在没有外源损伤的情况下促进 DNA 的快速复制。  |  Fu, H., et al. 2015. Nat Commun. 6: 6746. PMID: 25879486
  4. HIV-1 病毒蛋白 R (Vpr) 对 MUS81-EME1 蛋白的下调与 SLX4-SLX1 蛋白无关。  |  Zhou, X., et al. 2016. J Biol Chem. 291: 16936-16947. PMID: 27354282
  5. STC2是肝细胞癌中Mus81依赖性增殖和存活的新型介质。  |  Wu, F., et al. 2017. Cancer Lett. 388: 177-186. PMID: 27939696
  6. 通过激活 CHK1 通路，敲除 Mus81 可使结肠癌细胞对化疗药物敏感。  |  Wu, F., et al. 2017. Clin Res Hepatol Gastroenterol. 41: 592-601. PMID: 28291626
  7. MUS81 核酸酶活性对 BRCA2 缺陷细胞的复制压力耐受性和染色体分离至关重要。  |  Lai, X., et al. 2017. Nat Commun. 8: 15983. PMID: 28714477
  8. 有丝分裂过程中 DNA 复制中间产物的异常处理依赖于 Mus81-Eme1，会损害基因组的完整性。  |  Calzetta, NL., et al. 2020. Sci Adv. 6: PMID: 33298441
  9. 测定人类 MUS81-EME1&2 内切酶活性的方案。  |  Fang, Q., et al. 2022. STAR Protoc. 3: 101528. PMID: 35819885
  10. 下调 MUS81 的表达可抑制细胞迁移，并通过 miR-BART9-5p 维持 EBV 相关胃癌的潜伏感染。  |  Zhang, Y., et al. 2023. J Med Virol. 95: e28725. PMID: 37185865

订购信息

产品名称产品编号规格价格数量收藏夹

MUS81 抗体 (MTA30 2G10/3)

sc-53382
200 µg/ml
RMB2377.00

MUS81 (MTA30 2G10/3): m-IgG Fc BP-HRP 套装

sc-536949
200 µg Ab; 10 µg BP
RMB2662.00

MUS81 (MTA30 2G10/3): m-IgGκ BP-HRP 套装

sc-534126
200 µg Ab; 40 µg BP
RMB2662.00

MUS81 (MTA30 2G10/3): m-IgG2a BP-HRP 套装

sc-547907
200 µg Ab; 10 µg BP
RMB2662.00

What is the sequence of epitope is used for the antibody? I am looking for an antbody to detect a truncated MUS81.

Asked by: Jackie25
Thank you for your question. Anti-MUS81 Antibody (MTA30 2G10/3): sc-53382 was raised against Mus81 of human origin. No epitope mapping has been performed for this antibody as of yet, so the exact binding site is not known at this point.
Answered by: Tech Support Europe
Date published: 2021-11-02

What is the recommended starting dilution for Western blot for sc-53382: MUS81 (MTA30 2G10/3) monoclonal antibody?

Asked by: jenniferc
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Works fine for WBUsed on BJ-hTERT cells (lysed in NETN buffer). Worked well at 1:500 dilution, may want to use a lower dilution than this, however.
Date published: 2017-06-02
Rated 5 out of 5 by from Great for Western blotProduced specific results by WB with MUS81 Antibody (MTA30 2G10/3)
Date published: 2017-01-19
Rated 5 out of 5 by from This antibody worked wellThis antibody worked well.
Date published: 2015-05-29
Rated 5 out of 5 by from siRNA and WB publishable data with humansiRNA and WB publishable data with human Hela and Kyoto cells - SCBT Publication Review
Date published: 2015-05-29
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MUS81 抗体 (MTA30 2G10/3) is rated 5.0 out of 5 by 4.
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