Date published: 2025-9-7
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Mucin 4/MUC4 Antibody (1G8) - Western Blotting - Image 379159
Mucin 4 抗体 (1G8): sc-33654. HUV-EC-C (A), Y79 (B) 和 COLO 205 (C) 全细胞裂解液中 Mucin 4 表达的 Western 印迹分析.
Mucin 4 抗体 (1G8): sc-33654. HUV-EC-C (A), Y79 (B) 和 COLO 205 (C) 全细胞裂解液中 Mucin 4 表达的 Western 印迹分析.

Mucin 4/MUC4 抗体 (1G8): sc-33654

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说明书
  • Mucin 4/MUC4 抗体 1G8 是小鼠单克隆 IgG1 κ，Mucin 4/MUC4抗体, 在32篇文献中引用，规格为200 µg/ml
  • 针对大鼠粘蛋白4的Mucin 4跨膜域
  • Mucin 4/MUC4 抗体 (1G8) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 Mucin 4
  • 抗Mucin 4/MUC4抗体（1G8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Mucin 4/MUC4 (1G8): sc-33654的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 Mucin 4/MUC4 抗体 (1G8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Mucin 4/MUC4 抗体 (1G8) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Mucin 抗体 包括 Mucin 1, Mucin 12, Mucin 13, Mucin 15, Mucin 16, Mucin 2, Mucin 3, Mucin 4, Mucin 5AC和Mucin 5B

Mucin 4 Antibody (1G8) 是一种小鼠单克隆 IgG1 kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）检测小鼠、大鼠和人类来源的 Mucin 4 蛋白。Mucin 4 (1G8) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。Mucin 4 是一种重要的膜结合型糖蛋白，在细胞信号传导和上皮表面保护中发挥着重要作用。粘蛋白4由两个亚基组成：粘蛋白4α，一种大型氨基粘蛋白亚基；粘蛋白4β，一种含有三个表皮生长因子样结构域的跨膜亚基。这些结构域有助于与各种信号分子相互作用，从而影响细胞增殖和分化等过程。粘蛋白4主要在正常尿道上皮中表达，也存在于其他组织中，包括气管、肺和睾丸。Mucin 4表达失调与胰腺肿瘤有关，在胰腺肿瘤中，Mucin 4通过视黄酸受体-α信号通路上调，而TGFβ2则作为临时介质。Mucin 4基因经历选择性剪接，产生至少12种剪接变体，分为分泌型和膜结合型，进一步凸显了Mucin 4在正常生理和疾病状态下的复杂性和重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Mucin 4/MUC4 抗体 (1G8) 参考文献:

  1. 人类粘蛋白 MUC4 的完整序列：一种推定的细胞膜相关粘蛋白。  |  Moniaux, N., et al. 1999. Biochem J. 338 (Pt 2): 325-33. PMID: 10024507
  2. 交替剪接产生了一个假定分泌型和膜相关型 MUC4 粘蛋白家族。  |  Moniaux, N., et al. 2000. Eur J Biochem. 267: 4536-44. PMID: 10880978
  3. 视黄酸依赖性转化生长因子-β2介导的人胰腺肿瘤细胞 MUC4 粘蛋白表达诱导遵循视黄酸受体-α 信号通路。  |  Choudhury, A., et al. 2000. J Biol Chem. 275: 33929-36. PMID: 10938282
  4. 人体尿路上皮细胞和转入尿路的肠段中粘蛋白基因的表达。  |  N'Dow, J., et al. 2000. J Urol. 164: 1398-404. PMID: 10992422
  5. 巴雷特食管中黏蛋白基因的表达：原位杂交和免疫组化研究。  |  Arul, GS., et al. 2000. Gut. 47: 753-61. PMID: 11076872
  6. 人类正常, 癌前和恶性食管中粘蛋白基因的表达和细胞分化。  |  Guillem, P., et al. 2000. Int J Cancer. 88: 856-61. PMID: 11093805
  7. 人类胰腺肿瘤相关粘蛋白 MUC4 cDNA 3'-end 的交替剪接。  |  Choudhury, A., et al. 2001. Teratog Carcinog Mutagen. 21: 83-96. PMID: 11135323
  8. 人胆囊癌中 MUC1 和 MUC2 的表达：临床病理学研究及与预后的关系。  |  Kashiwagi, H., et al. 2001. Oncol Rep. 8: 485-9. PMID: 11295067
  9. 人类中耳和咽鼓管中粘蛋白的特征。  |  Lin, J., et al. 2001. Am J Physiol Lung Cell Mol Physiol. 280: L1157-67. PMID: 11350794
  10. MUCIN-4（MUC4）是胰腺癌免疫治疗中的一种新型肿瘤抗原。  |  Gautam, SK., et al. 2020. Semin Immunol. 47: 101391. PMID: 31952903
  11. 粘蛋白4通过激活AKT，使胆管癌患者对吉西他滨产生耐药性，并导致不良预后。  |  Pan, YR., et al. 2023. Int J Biol Sci. 19: 2772-2786. PMID: 37324940
  12. 致癌 KRAS、Mucin 4 和 Activin A 介导的成纤维细胞活化共同作用于 PanIN 的启动。  |  Hu, CM., et al. 2023. Adv Sci (Weinh). 10: e2301240. PMID: 37964407

订购信息

产品名称产品编号规格价格数量收藏夹

Mucin 4/MUC4 抗体 (1G8)

sc-33654
200 µg/ml
RMB2377.00

Mucin 4/MUC4 (1G8): m-IgG Fc BP-HRP 套装

sc-526697
200 µg Ab; 10 µg BP
RMB2662.00

Mucin 4/MUC4 (1G8): m-IgG1 BP-HRP 套装

sc-532070
200 µg Ab; 20 µg BP
RMB2662.00

Mucin 4/MUC4 抗体 (1G8) AC

sc-33654 AC
500 µg/ml, 25% agarose
RMB3129.00

Mucin 4/MUC4 抗体 (1G8) HRP

sc-33654 HRP
200 µg/ml
RMB2377.00

Mucin 4/MUC4 抗体 (1G8) FITC

sc-33654 FITC
200 µg/ml
RMB2482.00

Mucin 4/MUC4 抗体 (1G8) PE

sc-33654 PE
200 µg/ml
RMB2580.00

Mucin 4/MUC4 抗体 (1G8) Alexa Fluor® 488

sc-33654 AF488
200 µg/ml
RMB2685.00

Mucin 4/MUC4 抗体 (1G8) Alexa Fluor® 546

sc-33654 AF546
200 µg/ml
RMB2685.00

Mucin 4/MUC4 抗体 (1G8) Alexa Fluor® 594

sc-33654 AF594
200 µg/ml
RMB2685.00

Mucin 4/MUC4 抗体 (1G8) Alexa Fluor® 647

sc-33654 AF647
200 µg/ml
RMB2685.00

Mucin 4/MUC4 抗体 (1G8) Alexa Fluor® 680

sc-33654 AF680
200 µg/ml
RMB2685.00

Mucin 4/MUC4 抗体 (1G8) Alexa Fluor® 790

sc-33654 AF790
200 µg/ml
RMB2685.00

こちらの抗体の抗原の配列に関して伺いたいですが、教えていただけないでしょうか。

Asked by: omk9
Thank you for your question.Sc-33654 Mucin 4 (1G8) has not been epitope mapped. Here is a link for more information on the protein. https://www.uniprot.org/uniprotkb/Q8JZM8/entry
Answered by: Jiajun Z
Date published: 2025-08-08

I am planning to use Mucin 4/MUC4 Antibody (1G8): sc-33654-FITC for flow cytometry. In this case, which mouse IgG1 kappa light chain antibody should I use as a negative control? Is it okay to use any IgG1 FITC antibody?

Asked by: luximil
Thanks for your question. Normal mouse IgG1-FITC: sc-2855 is recommended for use as an isotype negative control immunoglobulin for flow cytometry application. 질문 감사합니다.정상 마우스 IgG1-FITC:sc-2855는 유세포측정기 적용을 위한 동형음성조절 면역글로불린으로 사용하는 것이 권장된다.
Answered by: Yanru Tao
Date published: 2024-11-04

Can I use your conjugated Mucin 4 antibody for flow cytometry or sorting?

Asked by: Bartha
Thank you for your question. Yes, the conjugated form on this mouse monoclonal Mucin 4 (1G8) antibody, sc-33654, is recommended for Flow cytometry.
Answered by: Tech Service
Date published: 2022-04-24

Mucin 4 Antibody (1G8): sc-33654 Do you sell the blocking peptide? Thank you!

Asked by: Roxana Schillaci
Thank you for your question. No, we do not offer a binding protein for this monoclonal antibody. We suggest using normal mouse IgG as a negative control.
Answered by: Tech Service
Date published: 2022-04-24

I would like to use Mucin 4 Antibody (1G8): sc-33654 for double staining with another mouse monoclonal primary antibody. Is this possible?

Asked by: Trav11
Thank you for your question. In cases like this, we recommend using our directly conjugated mouse monoclonal primary antibodies. Currently, sc-33654 is available conjugated to HRP, FITC, PE, Alexa Fluor® 488 and Alexa Fluor® 647. If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2022-04-24
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Rated 5 out of 5 by from Works in gerbils!Very pleased that this first Mucin 4 antibody I tried worked.
Date published: 2018-06-14
Rated 5 out of 5 by from Highly recommended for IHC(P) and WB.The go to monoclonal antibody for Mucin 4. Strong Immunoperoxidase staining of formalin fixed, paraffin-embedded human duodenum tissue. Well characterized in the primary literature.
Date published: 2017-01-07
Rated 5 out of 5 by from Publishable immunostaining in human paraffinPublishable immunostaining in human paraffin-embedded esphageal adenocarcinoma cells. -SCBT Publication Review
Date published: 2015-04-10
Rated 5 out of 5 by from Great antibody for WBGreat antibody for WB, works well in HUV-EC-C whole cell lysates. -SCBT QC
Date published: 2015-03-21
Rated 5 out of 5 by from Robust staining in paraffinRobust staining in paraffin-embedded human duodenum. -SCBT QC
Date published: 2015-01-24
Rated 5 out of 5 by from Great monoGreat mono! Successfully detects Mucin 4 activation in sc-400329-ACT transfected cells. -SCBT QC
Date published: 2015-01-04
Rated 5 out of 5 by from Good WB publishable data gathered with humanGood WB publishable data gathered with human gastric cancer cells. -SCBT Publication Review
Date published: 2015-01-02
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Mucin 4/MUC4 抗体 (1G8) is rated 5.0 out of 5 by 7.
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