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Monomethyl Histone H4 Antibody (5E10-D8) - Western Blotting - Image 377114 — 单甲基组蛋白 H4 抗体 (5E10-D8): sc-134221. 单甲基组蛋白 H4 在 HeLa 全细胞裂解液 (A) 和 K-562 (B), MCF7 (C), NIH/3T3 (D) 和 KNRK (E) 核提取物中表达的 Western 印迹分析.

单甲基组蛋白 H4 抗体 (5E10-D8): sc-134221. 单甲基组蛋白 H4 在 HeLa 全细胞裂解液 (A) 和 K-562 (B), MCF7 (C), NIH/3T3 (D) 和 KNRK (E) 核提取物中表达的 Western 印迹分析.

Monomethyl Histone H4 抗体 (5E10-D8): sc-134221

Name: Monomethyl Histone H4抗体 (5E10-D8)
Brand: SCBIO
SKU: sc-134221
Price: 2377.0 CNY
Availability: InStock

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说明书

Monomethyl Histone H4抗体(5E10-D8)是小鼠单克隆IgG₁ κ, 在6篇文献中引用，规格为200 µg/ml
针对human起源的Lys 20 monomethylated Histone H4中单甲基化的赖氨酸20的短氨基酸序列
抗-Monomethyl Histone H4 抗体 (5E10-D8) 推荐用于 WB, IP 和 ELISA，检测mouse, rat 和human 来源的 Lys 20 monomethylated Histone H4; 不会与 Histone H4 unmethylated or dimethylated at lysine 20, nor with Histone H3产生交联反应
抗Monomethyl Histone H4抗体（5E10-D8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
关于如何获取Monomethyl Histone H4 (5E10-D8): sc-134221的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG₁ BP-HRP和m-IgGκ BP-HRP是Monomethyl Histone H4 Antibody (5E10-D8) 适用于 WB 应用。的首选辅助检测试剂。这些试剂现与Monomethyl Histone H4 Antibody (5E10-D8) 打包提供（请参阅下面的订购信息）。

查看产品引用文献(6)

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関連項目

附加Histone抗体，包括Histone cluster 1 H1, Histone H2A, Histone H2B, Histone H3, Histone H4和Histone cluster 1 H3A

Monomethyl Histone H4抗体（5E10-D8）是一种IgG1 κ小鼠单克隆Monomethyl Histone H4抗体，它可以通过蛋白质印迹和酶联免疫吸附试验检测小鼠、大鼠和人类来源的Lys 20单甲基化Histone H4。Monomethyl Histone H4抗体（5E10-D8）既有非共轭形式的抗Monomethyl Histone H4抗体，也有多种共轭形式的抗Monomethyl Histone H4抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。真核生物的组蛋白是碱性的水溶性核蛋白，通过包裹146个碱基对的DNA并以左手超螺旋方式依次形成异八聚体核小体颗粒，进而构成染色体纤维。四个核心组蛋白（H2A、H2B、H3和H4）各由两个分子组成八聚体，其中包括两个H2A-H2B二聚体和两个H3-H4二聚体，通过三级结构形成两个几乎对称的半体。组蛋白主要受到酶的翻译后修饰，这些修饰主要发生在其N端尾部，但也存在于其球状结构域中。人类和小鼠的Histone H4在Lys 20位置会受到甲基化修饰，这种修饰对于特定的DNA交易或染色质状态转变可能是必要的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor^® 是Molecular Probes Inc., OR., USA的商标

LI-COR^®和 Odyssey^® 是LI-COR Biosciences的注册商标

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
Monomethyl Histone H4 抗体 (5E10-D8)	sc-134221	200 µg/ml	RMB2377.00
Monomethyl Histone H4 (5E10-D8): m-IgG Fc BP-HRP 套装	sc-528788	200 µg Ab; 10 µg BP	RMB2662.00
Monomethyl Histone H4 (5E10-D8): m-IgGκ BP-HRP 套装	sc-521296	200 µg Ab, 40 µg BP	RMB2662.00
Monomethyl Histone H4 (5E10-D8): m-IgG₁ BP-HRP 套装	sc-543131	200 µg Ab; 20 µg BP	RMB2662.00
Monomethyl Histone H4 抗体 (5E10-D8) AC	sc-134221 AC	500 µg/ml, 25% agarose	RMB3129.00
Monomethyl Histone H4 抗体 (5E10-D8) HRP	sc-134221 HRP	200 µg/ml	RMB2377.00
Monomethyl Histone H4 抗体 (5E10-D8) FITC	sc-134221 FITC	200 µg/ml	RMB2482.00
Monomethyl Histone H4 抗体 (5E10-D8) PE	sc-134221 PE	200 µg/ml	RMB2580.00
Monomethyl Histone H4 抗体 (5E10-D8) Alexa Fluor^® 488	sc-134221 AF488	200 µg/ml	RMB2685.00
Monomethyl Histone H4 抗体 (5E10-D8) Alexa Fluor^® 546	sc-134221 AF546	200 µg/ml	RMB2685.00
Monomethyl Histone H4 抗体 (5E10-D8) Alexa Fluor^® 594	sc-134221 AF594	200 µg/ml	RMB2685.00
Monomethyl Histone H4 抗体 (5E10-D8) Alexa Fluor^® 647	sc-134221 AF647	200 µg/ml	RMB2685.00
Monomethyl Histone H4 抗体 (5E10-D8) Alexa Fluor^® 680	sc-134221 AF680	200 µg/ml	RMB2685.00
Monomethyl Histone H4 抗体 (5E10-D8) Alexa Fluor^® 790	sc-134221 AF790	200 µg/ml	RMB2685.00

I need to use an antibody to detect the protein in samples of monkey origin. Do you know if sc-134221: Monomethyl Histone H4 (5E10-D8) mouse monoclonal antibody will work?

Asked by: DefinitelyNotMatt

Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.

Answered by: Technical Support

Date published: 2017-03-06

Rated 5 out of 5 by Shikata from This antibody is good for western blotting.I tested this antibody with preimplantaition embryos. It performs well in western blot analysis.

Date published: 2019-04-13

Rated 4 out of 5 by kathir from Works well for yeastI tested this antibody with the yeast protein extracts. It is sensitive and specific. I would recommend using this antibody in yeast.

Date published: 2017-10-27

Rated 5 out of 5 by LR from Publishable immunostaining in paraffin embeddedPublishable immunostaining in paraffin embedded mouse testes -SCBT Publication Review

Date published: 2015-04-08

Rated 5 out of 5 by LM from Clean blot with single strong band at expectedClean blot with single strong band at expected size using HeLa whole cell lysate. -SCBT QC

Date published: 2013-10-10

Monomethyl Histone H4 抗体 (5E10-D8) is rated 4.8 out of 5 by 4.

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Monomethyl Histone H4 抗体 (5E10-D8): sc-134221

Monomethyl Histone H4 抗体 (5E10-D8) 规格

替代产品名称: Monomethyl Histone H4

产品克隆名称: 5E10-D8

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG₁ kappa light chain

氨基酸: 16-26

抗原表位靶标: Lys 20 monomethylated Histone H4

抗原表位种属: human

产品反应性/检测: Lys 20 monomethylated Histone H4

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, ELISA

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 6篇各种科学出版物的引文