Date published: 2025-9-9
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MMP9 Antibody (2C3) - Western Blotting - Image 148927
MMP-9 抗体 (2C3): sc-21733. 人 PBL 全细胞裂解液中 MMP-9 表达的 Western 印迹分析.
MMP-9 抗体 (2C3): sc-21733. 人 PBL 全细胞裂解液中 MMP-9 表达的 Western 印迹分析.

MMP9 抗体 (2C3): sc-21733

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说明书
  • MMP9抗体(2C3)是小鼠单克隆IgG1 κ, 在325篇文献中引用，规格为200 µg/ml
  • 免疫human物种的MMP-9的氨基酸603-614
  • MMP9 抗体 (2C3) 推荐用于WB, IP, IF 和 IHC(P)，检测human 来源的 MMP-9
  • MMP9抗体（2C3）可与琼脂糖结合用于IP
  • 还可偶联Alexa Fluor® 594 用于IF, IHC(P) 和 FCM
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 看 MMP9 (E-11): sc-393859，可查看有标记的 MMP9 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取MMP9 (2C3): sc-21733的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 MMP9 抗体 (2C3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MMP9 抗体 (2C3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 MMP 抗体 包括 MMP-1, MMP-10, MMP-11, MMP-12, MMP-13, MMP-2, MMP-21, MMP-26, MMP-3和MMP-7

MMP-9抗体（2C3）是一种IgG1 κ小鼠单克隆MMP-9抗体，可通过WB、IP、IF和IHC(P)检测人源的MMP-9。MMP-9抗体（2C3）既可作为偶联形式也可作为非偶联形式的抗MMP-9抗体。基质金属蛋白酶（MMP）是一组肽酶，负责降解细胞外基质成分，包括胶原蛋白、明胶、纤维连接蛋白、层粘连蛋白和蛋白聚糖。MMP基因的转录被佛波酯、脂多糖（LPS）或葡萄球菌肠毒素B（SEB）差异激活。MMP催化需要钙和锌。MMP-9（也称为92 kDa IV型胶原酶或明胶酶B）已被证明与MMP-1（也称为间质胶原酶、成纤维细胞胶原酶或胶原酶-1）以及半胱氨酸蛋白酶协同降解骨胶原，并可能在骨破骨细胞吸收中发挥作用。p53可下调MMP-1的表达，p53表达异常可能通过调控MMP-1的表达参与类风湿关节炎的关节降解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MMP9 抗体 (2C3) 参考文献:

  1. p53 下调人类基质金属蛋白酶-1（Collagenase-1）基因的表达。  |  Sun, Y., et al. 1999. J Biol Chem. 274: 11535-40. PMID: 10206959
  2. 人类肿瘤细胞间质胶原酶的克隆和表征。  |  Templeton, NS., et al. 1990. Cancer Res. 50: 5431-7. PMID: 2167156
  3. 基质金属蛋白酶 9（92 千道尔顿明胶酶/IV 型胶原酶 = 明胶酶 B）在破骨细胞中的定位：对骨吸收的影响。  |  Okada, Y., et al. 1995. Lab Invest. 72: 311-22. PMID: 7898050
  4. 小鼠发育过程中破骨细胞系中 92-kD IV 型胶原酶（明胶酶 B）的高表达。  |  Reponen, P., et al. 1994. J Cell Biol. 124: 1091-1102. PMID: 8132709
  5. N末端在胶原酶 '超活化 '过程中作用的结构含义。晶体学研究。  |  Reinemer, P., et al. 1994. FEBS Lett. 338: 227-33. PMID: 8307185
  6. 基质金属蛋白酶：综述。  |  Birkedal-Hansen, H., et al. 1993. Crit Rev Oral Biol Med. 4: 197-250. PMID: 8435466
  7. 人类单核细胞中几种基质金属蛋白酶基因的表达。  |  Machein, U. and Conca, W. 1997. Adv Exp Med Biol. 421: 247-51. PMID: 9330704

订购信息

产品名称产品编号规格价格数量收藏夹

MMP9 抗体 (2C3)

sc-21733
200 µg/ml
RMB2377.00

MMP9 (2C3): m-IgG Fc BP-HRP 套装

sc-526603
200 µg Ab; 10 µg BP
RMB2662.00

MMP9 (2C3): m-IgG1 BP-HRP 套装

sc-531976
200 µg Ab; 20 µg BP
RMB2662.00

MMP9 抗体 (2C3) AC

sc-21733 AC
500 µg/ml, 25% agarose
RMB3129.00

MMP9 抗体 (2C3) Alexa Fluor® 546

sc-21733 AF546
200 µg/ml
RMB2685.00

MMP9 抗体 (2C3) Alexa Fluor® 594

sc-21733 AF594
200 µg/ml
RMB2685.00

MMP9 抗体 (2C3) Alexa Fluor® 680

sc-21733 AF680
200 µg/ml
RMB2685.00

MMP9 抗体 (2C3) Alexa Fluor® 790

sc-21733 AF790
200 µg/ml
RMB2685.00

Have there been reports of a cleaved form of this metalloproteinase? I'm getting a single clear concise band near 45 kDa in different cells lines.

Asked by: AnaL
Thank you for your question. We are not aware of any splicing variants that would appear at that molecular weight. Please reach out to our technical service so we can look into this matter.
Answered by: Tech Support Europe
Date published: 2020-11-16

Is any of MMP9 antibodies working with horses

Asked by: helsheikh
Thank you for your question. This antibody has not yet been tested in horse samples so that is not a recommended species at this time. We are still gathering data to show on our website. Check back for updates!
Answered by: Tech Service
Date published: 2019-11-06

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2016-12-21
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Rated 4 out of 5 by from Great for WBThe antibody is good for western blot. The band is single and clear.
Date published: 2024-04-01
Rated 4 out of 5 by from Good for ImmunofluorescenceThe antibody works very well in immunofluorescence (1:200) on mesenchymal stem cells fixed with 4% PFA.
Date published: 2020-11-10
Rated 3 out of 5 by from みえなかった。サンプルでいただきました。癌細胞を使ってウエスタンブロットで確認。濃度は濃く使用しましたが、検出されませんでした。
Date published: 2020-08-19
Rated 5 out of 5 by from Great for immunofluorescenceI purchased this Ab about a month ago and have had great results in my immunofluorescence staining.
Date published: 2019-11-06
Rated 1 out of 5 by from very faint band at 1:250 dilutionI received the antibody to test on PMA-stimulated THP-1 cells, the 90kDa band is faint and there is unspecific band approx. 45kDa
Date published: 2019-04-25
Rated 5 out of 5 by from 目标条带清晰在肠癌和乳腺癌的wb中，这支抗体有清晰的目标条带，未出现杂带，背景清晰，适合在wb中使用，稀释比例1:500
Date published: 2018-04-02
Rated 5 out of 5 by from Excellent productI got a free sample of this product last month and it work very well for my samples: cell line and tumor from mice
Date published: 2018-02-08
Rated 5 out of 5 by from western blotA clear and strong signal was showed in the lysis of HepG2 cells (1:400).
Date published: 2018-01-25
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MMP9 抗体 (2C3) is rated 4.6 out of 5 by 21.
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