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MMP-12 Antibody (G-2) - Western Blotting - Image 362899
MMP-12 抗体 (G-2): sc-390863. 在 AMJ2-C8 (A), SW480 (B), RAW 264. 7 (C) 和 A-10 (D) 全细胞裂解液以及大鼠肺组织提取物 (E) 中 MMP-12 表达的 Western 印迹分析.
MMP-12 抗体 (G-2): sc-390863. 在 AMJ2-C8 (A), SW480 (B), RAW 264. 7 (C) 和 A-10 (D) 全细胞裂解液以及大鼠肺组织提取物 (E) 中 MMP-12 表达的 Western 印迹分析.

MMP-12 抗体 (G-2): sc-390863

5.0(1)
寫評論提問

说明书
  • MMP-12抗体(G-2)是小鼠单克隆IgG1 κ, 在14篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于mouse物种的MMP-12的C-terminus附近的氨基酸438-453之间
  • 抗-MMP-12 抗体 (G-2) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 MMP-12
  • 抗MMP-12抗体（G-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MMP-12 (G-2): sc-390863的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是MMP-12 Antibody (G-2) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与MMP-12 Antibody (G-2) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加MMP抗体，包括MMP-1, MMP-10, MMP-11, MMP-12, MMP-13, MMP-2, MMP-21, MMP-26, MMP-3, MMP-7, MMP-8和MMP-9

MMP-12抗体（G-2）是一种IgG1 κ小鼠单克隆MMP-12抗体，可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的MMP-12。MMP-12抗体（G-2）既可作为非偶联的抗MMP-12抗体形式提供，也可作为多种偶联形式的抗MMP-12抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。基质金属蛋白酶（MMP）是一类肽酶，负责降解细胞外基质成分，包括胶原蛋白、明胶、纤连蛋白、层粘连蛋白和蛋白聚糖。MMP基因的转录被佛波酯、脂多糖（LPS）或金黄色葡萄球菌肠毒素B（SEB）差异性激活。MMP的催化需要钙和锌。MMP-12（也称为巨噬细胞金属弹性蛋白酶）在肺泡巨噬细胞中产生，并降解弹性蛋白。MMP-12可能有助于肉芽肿性皮肤病中发生的弹性蛋白降解，并可能参与巨噬细胞在炎症性疾病中通过表皮和血管基膜的迁移。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MMP-12 抗体 (G-2) 参考文献:

  1. 人金属弹性蛋白酶（MMP-12）在皮肤肉芽肿和巨噬细胞迁移中的表达增强。  |  Vaalamo, M., et al. 1999. J Invest Dermatol. 112: 499-505. PMID: 10201535
  2. 小鼠巨噬细胞金属弹性蛋白酶的分子克隆, 染色体定域和细菌表达。  |  Shapiro, SD., et al. 1992. J Biol Chem. 267: 4664-71. PMID: 1537850
  3. 将巨噬细胞金属弹性蛋白酶（MMP-12）作为治疗呼吸系统炎症性疾病的靶点。  |  Lagente, V., et al. 2009. Expert Opin Ther Targets. 13: 287-95. PMID: 19236151
  4. 有症状和无症状重度颈动脉狭窄患者的 MMP-12 和 TIMP 行为。  |  Del Porto, F., et al. 2017. J Stroke Cerebrovasc Dis. 26: 334-338. PMID: 27746079
  5. MMP-1、MMP-3和MMP-12与动脉粥样硬化程度、斑块稳定性及心脑血管事件的相关性。  |  Hu, W., et al. 2018. Exp Ther Med. 15: 1994-1998. PMID: 29434795
  6. 口腔黏膜下纤维化和口腔鳞状细胞癌患者唾液基质金属蛋白酶-12（MMP- 12）水平的估计。  |  Saleem, Z., et al. 2021. BMC Oral Health. 21: 205. PMID: 33892690
  7. RSV感染诱发的巨噬细胞可产生大量MMP-12，加剧过敏性气道炎症和中性粒细胞浸润。  |  Makino, A., et al. 2021. iScience. 24: 103201. PMID: 34703996
  8. 将循环 MMP-12 作为评估过敏性鼻炎疾病严重程度和舌下免疫疗法疗效的潜在生物标记物  |  Zhou, Y., et al. 2022. Mediators Inflamm. 2022: 3378035. PMID: 35733520
  9. 人肺泡巨噬细胞产生的一种独特的弹性溶解金属蛋白酶的克隆和表征。  |  Shapiro, SD., et al. 1993. J Biol Chem. 268: 23824-9. PMID: 8226919
  10. N末端在胶原酶 '超活化 '过程中作用的结构含义。晶体学研究。  |  Reinemer, P., et al. 1994. FEBS Lett. 338: 227-33. PMID: 8307185
  11. 基质金属蛋白酶：综述。  |  Birkedal-Hansen, H., et al. 1993. Crit Rev Oral Biol Med. 4: 197-250. PMID: 8435466
  12. 人类单核细胞中几种基质金属蛋白酶基因的表达。  |  Machein, U. and Conca, W. 1997. Adv Exp Med Biol. 421: 247-51. PMID: 9330704

订购信息

产品名称产品编号规格价格数量收藏夹

MMP-12 抗体 (G-2)

sc-390863
200 µg/ml
RMB2377.00

MMP-12 (G-2): m-IgG Fc BP-HRP 套装

sc-530292
200 µg Ab; 10 µg BP
RMB2662.00

MMP-12 (G-2): m-IgGκ BP-HRP 套装

sc-523715
200 µg Ab, 40 µg BP
RMB2662.00

MMP-12 (G-2): m-IgG1 BP-HRP 套装

sc-543975
200 µg Ab; 20 µg BP
RMB2662.00

MMP-12 抗体 (G-2) AC

sc-390863 AC
500 µg/ml, 25% agarose
RMB3129.00

MMP-12 抗体 (G-2) HRP

sc-390863 HRP
200 µg/ml
RMB2377.00

MMP-12 抗体 (G-2) FITC

sc-390863 FITC
200 µg/ml
RMB2482.00

MMP-12 抗体 (G-2) PE

sc-390863 PE
200 µg/ml
RMB2580.00

MMP-12 抗体 (G-2) Alexa Fluor® 488

sc-390863 AF488
200 µg/ml
RMB2685.00

MMP-12 抗体 (G-2) Alexa Fluor® 546

sc-390863 AF546
200 µg/ml
RMB2685.00

MMP-12 抗体 (G-2) Alexa Fluor® 594

sc-390863 AF594
200 µg/ml
RMB2685.00

MMP-12 抗体 (G-2) Alexa Fluor® 647

sc-390863 AF647
200 µg/ml
RMB2685.00

MMP-12 抗体 (G-2) Alexa Fluor® 680

sc-390863 AF680
200 µg/ml
RMB2685.00

MMP-12 抗体 (G-2) Alexa Fluor® 790

sc-390863 AF790
200 µg/ml
RMB2685.00

MMP-12 (G-2) 中和勝肽

sc-390863 P
100 µg/0.5 ml
RMB511.00

Can this product be purchased without azide? I would like to try it as a neutralizing antibody with live cells.

Asked by: Beez
Thank you for your question. Yes, this product can be purchased without azide, please contact technical service for further assistance.
Answered by: Technical Service 4
Date published: 2020-12-07

Does this antibody target the pro-form or active form of Mmp12 or both?

Asked by: Zhipeng
Thank you for your question. The zymogen form for MMP-12 is about ~54 kDa, which is activated to the 45-48 kDa form, which further breaks down to a cascade of active forms and resulting in a common 22 kDa form. Which form you will see depends on what form is present in your sample. Our data with this antibody generally shows the active 48kDa form.
Answered by: Tech Service
Date published: 2019-10-08

Dear Madam/Sir, I am write in asking for how to receive FREE 10 µg sample of MMP-12 (G-2): sc-390863. I want to give a try before buying some for my research

Asked by: YuanCao
Thank you for your question. Please contact one of our authorized distributors in Australia to request a free 10 µg sample of this monoclonal antibody. Please use the link below to search for their contact information on our website. https://www.scbt.com/scbt/resources/distributors
Answered by: Technical Support
Date published: 2017-10-12

What application is the blocking peptide sc-390863 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Specific and strong band detected by WesternSpecific and strong band detected by Western blot analysis of MMP-12 expression in AMJ2-C8 whole cell lysate. - SCBT QC
Date published: 2015-03-06
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MMP-12 抗体 (G-2) is rated 5.0 out of 5 by 1.
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