TEST CONDITIONS
HPLC Conditions: Column: C18, 5μ, 4.6 X 250 mm, Mob. Phase: Methanol: Water: Acetic acid (80: 20: 0.1), Flow Rate: 1.00 ml/min, Detector UV: 289 nm. TLC Conditions: Mob. Phase: Hexane: Ethyl Acetate (80: 20), Stationary Phase: Silica Gel, Visualized With: Sulfuric Acid.