Date published: 2025-9-8
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MKLP-1 Antibody (C-12) - Western Blotting - Image 286106
MKLP-1 抗体 (C-12): sc-390113. K-562 (A), HeLa (B) 和 Jurkat (C) 全细胞裂解液中 MKLP-1 表达的 Western 印迹分析.
MKLP-1 抗体 (C-12): sc-390113. K-562 (A), HeLa (B) 和 Jurkat (C) 全细胞裂解液中 MKLP-1 表达的 Western 印迹分析.

MKLP-1 抗体 (C-12): sc-390113

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说明书
  • MKLP-1 抗体 C-12 是小鼠单克隆 IgG1 κ，MKLP-1抗体, 在9篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1-110位于human物种的MKLP-1的N-terminus
  • MKLP-1 抗体 (C-12) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 MKLP-1
  • 抗MKLP-1抗体（C-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MKLP-1 (C-12): sc-390113的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是MKLP-1 Antibody (C-12) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与MKLP-1 Antibody (C-12) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
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研究信息

関連項目

MKLP-1抗体（C-12）是一种IgG1 κ小鼠单克隆MKLP-1抗体（也称为有丝分裂驱动蛋白样蛋白1（MKLP1）抗体、驱动蛋白样蛋白家族成员6抗体、驱动蛋白-6抗体、微管依赖性分子马达相关抗体、有丝分裂正端定向马达蛋白抗体、后期中央纺锤体复合体相关抗体或KNSL5抗体），可通过WB、IP、IF和ELISA检测人源MKLP-1蛋白。MKLP-1抗体（C-12）可以以非偶联抗MKLP-1抗体的形式提供，也可以以多种偶联形式的抗MKLP-1抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。单克隆抗体CHO1检测有丝分裂进程中所需的纺锤体抗原。用该抗体筛选HeLa细胞cDNA表达文库已显示出能产生一种cDNA，该cDNA预计能编码一种在其氨基末端半部与驱动蛋白超家族成员的马达端显著相关的蛋白质。由于这种相似性并没有进一步扩展，因此有人提出，现在被指定为MKLP-1（有丝分裂驱动蛋白样蛋白-1）的CHO1抗原代表一种新型驱动蛋白。序列分析还表明，MKLP-1具有核蛋白的典型特征。免疫细胞学研究表明，MKLP-1在有丝分裂早期从细胞核中移出，然后在细胞质分裂后移至中间体。MKLP-1已被证明能在体外捆绑反平行微管，并以与体内纺锤体伸长相当的速度移动它们。还分离出了MKLP-1的仓鼠同源物，被指定为CHO1抗原。尽管在微管捆绑活性方面看似功能相当，但人蛋白和仓鼠蛋白在其C-末端存在显著差异，这可能是由于选择性剪接或存在多个MKLP-1位点所致。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MKLP-1 抗体 (C-12) 参考文献:

  1. 驱动蛋白样蛋白 MKLP-1 C 端结构域的核定位  |  Deavours, BE. and Walker, RA. 1999. Biochem Biophys Res Commun. 260: 605-8. PMID: 10403813
  2. 一种能在体外移动反平行微管的加端定向马达酶定位在有丝分裂纺锤体的区间。  |  Nislow, C., et al. 1992. Nature. 359: 543-7. PMID: 1406973
  3. Polo-like-kinase 1 与有丝分裂驱动蛋白 CHO1/MKLP-1 的分子相互作用  |  Liu, X., et al. 2004. J Cell Sci. 117: 3233-46. PMID: 15199097
  4. 有丝分裂纺锤体的分子成分  |  Kuriyama, R. and Nislow, C. 1992. Bioessays. 14: 81-8. PMID: 1575716
  5. MKLP1 的树突靶向需要特定的结构域。  |  Xu, X., et al. 2006. J Cell Sci. 119: 452-8. PMID: 16418225
  6. 有丝分裂驱动蛋白样蛋白 Mklp-1 的核定位信号：细胞分裂过程中对 Mklp-1 功能的影响  |  Liu, X. and Erikson, RL. 2007. Biochem Biophys Res Commun. 353: 960-4. PMID: 17198681
  7. 一种有丝分裂微管相关蛋白的单克隆抗体会阻碍有丝分裂的进行。  |  Nislow, C., et al. 1990. J Cell Biol. 111: 511-22. PMID: 2199459
  8. 中国仓鼠卵巢细胞中体基质成分在细胞周期中的分布。  |  Sellitto, C. and Kuriyama, R. 1988. J Cell Biol. 106: 431-9. PMID: 2448315
  9. 核定位信号介导的蛋白质运输  |  Roberts, B. 1989. Biochim Biophys Acta. 1008: 263-80. PMID: 2667641
  10. CYK4 可以放松 MKLP1 驱动蛋白-6 在离轴运动中的偏差。  |  Maruyama, Y., et al. 2021. Commun Biol. 4: 180. PMID: 33568771
  11. 有丝分裂特异性驱动蛋白 CHO1 抗原的异质性与微管相互作用。分析在昆虫 sf9 细胞中表达的亚域。  |  Kuriyama, R., et al. 1994. J Cell Sci. 107 (Pt 12): 3485-99. PMID: 7706400
  12. 向谢赫拉扎德致歉：1001 个驱动蛋白马达的尾巴。  |  Goldstein, LS. 1993. Annu Rev Genet. 27: 319-51. PMID: 8122906

订购信息

产品名称产品编号规格价格数量收藏夹

MKLP-1 抗体 (C-12)

sc-390113
200 µg/ml
RMB2377.00

MKLP-1 (C-12): m-IgG Fc BP-HRP 套装

sc-530067
200 µg Ab; 10 µg BP
RMB2662.00

MKLP-1 (C-12): m-IgGκ BP-HRP 套装

sc-523411
200 µg Ab, 40 µg BP
RMB2662.00

MKLP-1 (C-12): m-IgG1 BP-HRP 套装

sc-543852
200 µg Ab; 20 µg BP
RMB2662.00

MKLP-1 抗体 (C-12) AC

sc-390113 AC
500 µg/ml, 25% agarose
RMB3129.00

MKLP-1 抗体 (C-12) HRP

sc-390113 HRP
200 µg/ml
RMB2377.00

MKLP-1 抗体 (C-12) FITC

sc-390113 FITC
200 µg/ml
RMB2482.00

MKLP-1 抗体 (C-12) PE

sc-390113 PE
200 µg/ml
RMB2580.00

MKLP-1 抗体 (C-12) Alexa Fluor® 488

sc-390113 AF488
200 µg/ml
RMB2685.00

MKLP-1 抗体 (C-12) Alexa Fluor® 546

sc-390113 AF546
200 µg/ml
RMB2685.00

MKLP-1 抗体 (C-12) Alexa Fluor® 594

sc-390113 AF594
200 µg/ml
RMB2685.00

MKLP-1 抗体 (C-12) Alexa Fluor® 647

sc-390113 AF647
200 µg/ml
RMB2685.00

MKLP-1 抗体 (C-12) Alexa Fluor® 680

sc-390113 AF680
200 µg/ml
RMB2685.00

MKLP-1 抗体 (C-12) Alexa Fluor® 790

sc-390113 AF790
200 µg/ml
RMB2685.00

Can the MKLP-1 (C-12) monoclonal antibody recognise MKLP-1 of mouse origin or just human? It isn't clear from the details provided. Thank you

Asked by: CellDeathLab
Thank you for your question. MKLP-1 (C-12) is recommended for detection of MKLP-1 of human origin by WB, IP, IF and ELISA.
Answered by: Technical Support 12
Date published: 2021-07-05

The MKLP N-19 is has been discontinued. It worked very well in IF, it stained spindle midzone very clearly with almost no background. I wonder if the C-12 monoclonal also work in IF? If yes, can I see any example?

Asked by: GaryCrick
Thank you for your question. sc-390113 MKLP-1 (C-12) is recommended and warrantied for IF use on human samples. Unfortunately I do not have any published images we can provide at this time. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-09-06

Can MKLP-1 (C-12): sc-390113 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, MKLP-1 (C-12): sc-390113 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 良かったです。western blotでは500倍希釈で問題なく使用できました。 試しに免疫染色を試みましたが、発色は得られませんでした。
Date published: 2017-12-19
Rated 4 out of 5 by from Try both MKLP-1 antibodiesTried both the N24 and the C-12 antibody for MKLP-1 and one does work better although I am not sure which antibody is the more specific.
Date published: 2017-11-17
Rated 1 out of 5 by from Doesn't work in IFI asked for a free sample to try as I previosuly used N-19 which has been discontinued. But when I fixed cell with 4% paraformaldehyde, it does not work in IF.
Date published: 2017-09-19
Rated 5 out of 5 by from Produced positive Western blot data of MKLPProduced positive Western blot data of MKLP-1 expression in K-562, HeLa and Jurkat whole cell lysates. -SCBT QC
Date published: 2013-07-16
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MKLP-1 抗体 (C-12) is rated 3.8 out of 5 by 4.
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