Date published: 2025-9-7

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Mitofilin 抗体 (D-3): sc-390707

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说明书
  • Mitofilin 抗体 D-3 是小鼠单克隆 IgG1 κ,Mitofilin抗体, 在8篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列364-657位于human物种的Mitofilin的内部区域内
  • Mitofilin 抗体 (D-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Mitofilin
  • 抗Mitofilin抗体(D-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Mitofilin (D-3): sc-390707免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 Mitofilin 抗体 (D-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Mitofilin 抗体 (D-3) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Mitofilin抗体(D-3)是一种IgG1 κ小鼠单克隆Mitofilin抗体(也称为Mitofilin抗体),可通过WB、IP、IF、IHC(P)和ELISA检测来自小鼠、大鼠和人类的Mitofilin蛋白。Mitofilin抗体(D-3)既有非偶联抗Mitofilin抗体形式,也有多种偶联形式的抗Mitofilin抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。嵴是高度折叠的线粒体内膜隔室,上面镶嵌着细胞色素和ATP合酶蛋白,功能是提供一个大的表面积以供细胞呼吸发生。线粒体蛋白(也称为IMMT、HMP、PIG4或P87/89)是一种由758个氨基酸组成的线粒体内膜蛋白,优先表达于心脏组织中。线粒体蛋白存在三种选择性剪接的异构体,其功能是控制线粒体嵴的形态,包括嵴连接的形成和组织以及正常的管状嵴。由于线粒体蛋白在介导嵴的结构和功能中发挥作用,因此它对正常的线粒体功能至关重要,从而对整个细胞的存活也至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Mitofilin 抗体 (D-3) 参考文献:

  1. 线粒体内膜蛋白 mitofilin 控制着嵴的形态。  |  John, GB., et al. 2005. Mol Biol Cell. 16: 1543-54. PMID: 15647377
  2. 黑色素瘤相关视网膜病变中作为靶抗原的米托菲林和泰汀。  |  Pföhler, C., et al. 2007. Int J Cancer. 120: 788-95. PMID: 17131336
  3. 线粒体内膜蛋白 mitofilin 与 SAM50, metaxins 1 和 2, coil-coil-helix coiling-helix domain-containing protein 3 和 6 以及 DnaJC11 组成复合物。  |  Xie, J., et al. 2007. FEBS Lett. 581: 3545-9. PMID: 17624330
  4. 鉴定脑外伤后多 ADP 核糖基化线粒体蛋白。  |  Lai, Y., et al. 2008. J Neurochem. 104: 1700-11. PMID: 17996029
  5. 衰老加速小鼠海马体蛋白质组分析:Uchl3 和 mitofilin 对认知障碍和 SAMP8 线粒体功能障碍的影响  |  Wang, Q., et al. 2008. Neurochem Res. 33: 1776-82. PMID: 18307031
  6. 载脂蛋白的新细胞内功能:作为线粒体蛋白/MINOS复合物成分的ApoO蛋白家族决定了线粒体的嵴形态。  |  Koob, S. and Reichert, AS. 2014. Biol Chem. 395: 285-96. PMID: 24391192
  7. 线粒体蛋白的下调抑制了HeLa细胞的自动吞噬,并促进了饥饿诱导的细胞凋亡。  |  Shen, M., et al. 2019. Iran J Basic Med Sci. 22: 1132-1137. PMID: 31998453
  8. CHCHD10调控的OPA1-mitofilin复合体介导了TDP-43诱导的与额颞叶痴呆症相关的线粒体表型。  |  Liu, T., et al. 2020. FASEB J. 34: 8493-8509. PMID: 32369233
  9. Parkin 与 Mitofilin 相互作用,增加多巴胺能神经元在帕金森病相关压力下的死亡。  |  Imam Aliagan, AD., et al. 2020. Am J Transl Res. 12: 7542-7564. PMID: 33312388
  10. 一种在心肌中优先转录的新型人类基因。  |  Icho, T., et al. 1994. Gene. 144: 301-6. PMID: 8039717
  11. 丝裂蛋白(HMP)的分子特征,丝裂蛋白是一种线粒体缔合作用蛋白,具有预测的盘绕线圈和膜间空间靶向结构域。  |  Odgren, PR., et al. 1996. J Cell Sci. 109 (Pt 9): 2253-64. PMID: 8886976
  12. Mitofilin 是线粒体内膜的一种跨膜蛋白,以两种异构体形式表达。  |  Gieffers, C., et al. 1997. Exp Cell Res. 232: 395-9. PMID: 9168817

订购信息

产品名称产品编号规格价格数量收藏夹

Mitofilin 抗体 (D-3)

sc-390707
200 µg/ml
RMB2377.00

Mitofilin (D-3): m-IgG Fc BP-HRP 套装

sc-530235
200 µg Ab; 10 µg BP
RMB2662.00

Mitofilin (D-3): m-IgGκ BP-HRP 套装

sc-523646
200 µg Ab, 40 µg BP
RMB2662.00

Mitofilin (D-3): m-IgG1 BP-HRP 套装

sc-543945
200 µg Ab; 20 µg BP
RMB2662.00

Mitofilin 抗体 (D-3) AC

sc-390707 AC
500 µg/ml, 25% agarose
RMB3129.00

Mitofilin 抗体 (D-3) HRP

sc-390707 HRP
200 µg/ml
RMB2377.00

Mitofilin 抗体 (D-3) FITC

sc-390707 FITC
200 µg/ml
RMB2482.00

Mitofilin 抗体 (D-3) PE

sc-390707 PE
200 µg/ml
RMB2580.00

Mitofilin 抗体 (D-3) Alexa Fluor® 488

sc-390707 AF488
200 µg/ml
RMB2685.00

Mitofilin 抗体 (D-3) Alexa Fluor® 546

sc-390707 AF546
200 µg/ml
RMB2685.00

Mitofilin 抗体 (D-3) Alexa Fluor® 594

sc-390707 AF594
200 µg/ml
RMB2685.00

Mitofilin 抗体 (D-3) Alexa Fluor® 647

sc-390707 AF647
200 µg/ml
RMB2685.00

Mitofilin 抗体 (D-3) Alexa Fluor® 680

sc-390707 AF680
200 µg/ml
RMB2685.00

Mitofilin 抗体 (D-3) Alexa Fluor® 790

sc-390707 AF790
200 µg/ml
RMB2685.00

请问抗体浓度是多少ug/ml?用AG磁珠做IP稀释比例是多少呢?

Asked by: Anya1991
Thanks for your question. Anti-Mitofilin Antibody (D-3): sc-390707 is provided at 200 µg/ml. To 1 ml of cell lysate, or approximately 100–1000 µg of total cellular protein,incubate lysate with 1–10 µl (i.e., 0.2–2 µg) primary antibody (optimal antibody concentration should be determined by titration) . 感谢您的提问。Anti-Mitofilin 抗体(D-3): sc-390707浓度是200 µg/ml。对于1ml细胞裂解液或100-1000 µg全细胞蛋白,建议使用1-10ul一抗(最佳抗体浓度需要进行浓度滴定)。
Answered by: Yanru Tao
Date published: 2022-06-09

I am using Mitofilin (D-3): sc-390707 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-25
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Rated 4 out of 5 by from Good antibody in WBWe have tested in human proximal tubule cell extract and it worked well
Date published: 2020-12-03
Rated 4 out of 5 by from Very clear WB in pancreatic cell linesGive us a clear band at expected size in 4 pancreatic cell lines : Panc-1, BxPC-3, HPAF-2, MiaPaCa-2 and Patu8988T
Date published: 2018-05-15
Rated 5 out of 5 by from Great antibodyThis antibody is very effective in western blot. I can get a strong signal band in 293T cells.
Date published: 2018-04-10
Rated 5 out of 5 by from 出色试用了一下小样,结果很是出乎意料。条带干净,特异性好。很灵敏。值得购买。所以我又联系了其他同学购买santa cruz的抗体产品。
Date published: 2017-10-23
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmicProduced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human heart muscle tissue. -SCBT QC
Date published: 2015-03-17
Rated 5 out of 5 by from Produced positive Western Blot data of MitofilinProduced positive Western Blot data of Mitofilin expression in Raji and HeLa whole cell lysates. -SCBT QC
Date published: 2013-05-09
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Mitofilin 抗体 (D-3) is rated 4.7 out of 5 by 6.
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