Date published: 2025-9-8
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MITF Antibody (4H205) - Western Blotting - Image 143379
MITF 抗体 (4H205): sc-71587. Jurkat (A) 和 SK-MEL-28 (B) 核提取物中 MITF 表达的 Western 印迹分析.
MITF Antibody (4H205) - Western Blotting - Image 143379
MITF 抗体 (4H205): sc-71587. Jurkat (A) 和 SK-MEL-28 (B) 核提取物中 MITF 表达的 Western 印迹分析.
MITF Antibody (4H205) - Western Blotting - Image 38612
MITF 抗体 (4H205): sc-71587. Western 印迹分析 Jurkat 核提取物中 MITF 的表达.
MITF 抗体 (4H205): sc-71587. Jurkat (A) 和 SK-MEL-28 (B) 核提取物中 MITF 表达的 Western 印迹分析.

MITF 抗体 (4H205): sc-71587

4.3(3)
寫評論提問

说明书
  • MITF 抗体 4H205 是小鼠单克隆 IgG1, 在1篇文献中引用，提供 100 µg/ml
  • 针对氨基酸408-419 MITF human
  • 推荐WB 和 IP检测mouse, rat, human, avian 和 Xenopus来源的MITF; 也和以下物种反应，包括： 和 equine, bovine and canine
  • 目前，我们还没有完成MITF 抗体 (4H205)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

MITF（小眼畸形相关转录因子）是一种含有碱性螺旋-环-螺旋（HLH）和亮氨酸拉链（LZ）结构域的黑素细胞核蛋白。这些蛋白质基序在其他转录因子中经常被观察到，在Myc家族成员中尤为常见。 MITF可作为同二聚体直接与DNA结合，是黑素细胞发育和分化的必要条件。其表达由cAMP和cAMP依赖性途径上调。 MITF通过结合其E-box激活几种不同的基因启动子。酪氨酸酶、TRP1和TRP2是MITF激活的色素合成基因。当Ser73上的MITF被磷酸化时（通过MAPK途径），它与p300/CBP家族的共激活因子结合并增强转录。 MITF具有几种同工型，包括在黑素细胞中特异性表达的MITF-M。在MITF缺陷小鼠中，黑素细胞完全缺失。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

MITF 抗体 (4H205)

sc-71587
100 µg/ml
RMB2377.00

Do you sell a small size of this MiTF Ab (sc-71587)?

Asked by: ND_WCM
Thank you for your question. No, we do not sell a smaller size. The smallest size available for this antibody is 100 ug in 1 ml. We do offer some free 10 ug sample sizes for alternative MITF antibodies, including: sc-515925, sc-52938 and sc-56726. Please contact our Technical Service Department for more information on how to receive a free 10 ug samples of monoclonal antibodies.
Answered by: Technical Support
Date published: 2019-07-24

For Western Blot, is it recommended to use denatured or non-denatured conditions with MITF (4H205): sc-71587 antibody?

Asked by: Professor Griffin
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-03-27
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Rated 5 out of 5 by from good results at correct molecular weightWe used this antibody for a western blot experiment and got nice dark bands in Jurkat cells at the correct molecular weight
Date published: 2017-01-19
Rated 3 out of 5 by from Faint positive signal in western blot usingFaint positive signal in western blot using Jurkat whole cell lysates. -SCBT QC
Date published: 2015-05-27
Rated 5 out of 5 by from StrongStrong, clean bands in western blot analysis of MITF expression in Jurkat and SK-MEL-28 nuclear extracts. -SCBT QC
Date published: 2014-03-08
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MITF 抗体 (4H205) is rated 4.3 out of 5 by 3.
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