Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
MIF Antibody (D-2) - Western Blotting - Image 135217
MIF 抗体 (D-2): sc-271631. Western 印迹分析 Jurkat 全细胞裂解液中 MIF 的表达.
MIF 抗体 (D-2): sc-271631. Western 印迹分析 Jurkat 全细胞裂解液中 MIF 的表达.

MIF 抗体 (D-2): sc-271631

4.4(11)
寫評論提問

说明书
  • MIF 抗体 D-2 是小鼠单克隆 IgM κ，MIF抗体, 在15篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的MIF的N-terminus的氨基酸7-39之间
  • 推荐用于 mouse, rat 和 human 来源的MIF WB, IP, IF, IHC(P) 和 ELISA检测; 也和以下物种反应，包括： porcine
  • 另提供琼脂糖偶联抗体，规格为琼脂糖装，可用于IP实验；HRP 偶联抗体用于WB, IHC(P)和ELISA实验；以及藻红蛋白、FITC、Alexa Fluor® 488 或Alexa Fluor® 647 偶联抗体，规格为用于IF、IHC(P)和FCM实验
  • 关于如何获取MIF (D-2): sc-271631的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成MIF 抗体 (D-2)的首选二抗检测试剂的鉴定。这项工作正在进行中。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

MIF抗体（D-2）是一种IgM κ小鼠单克隆MIF抗体（也称为GLIF抗体或苯丙酮酸互变异构酶抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类的MIF蛋白。MIF抗体（D-2）既可以非偶联的抗MIF抗体形式提供，也可以多种偶联形式的抗MIF抗体形式提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。巨噬细胞迁移抑制因子，即MIF或糖基化抑制因子，是一种分泌的、同三聚体的促炎细胞因子，可调节巨噬细胞和T细胞的功能，是宿主对感染反应的重要调节因子。MIF在炎症部位表达，这表明它在调节宿主防御中的巨噬细胞功能方面发挥作用。MIF由垂体产生，存在于单核细胞、巨噬细胞、眼晶状体和脑中的分化免疫细胞以及成纤维细胞中。在严重脓毒症或感染性休克患者的血浆中可检测到MIF蛋白水平升高，在这种情况下，MIF通过增强其他炎性细胞因子（包括肿瘤坏死因子α（TNFα）、白细胞介素-1（IL-1）和干扰素-γ（IFN-γ））的产生来影响内毒素休克。MIF通过拮抗糖皮质激素介导的免疫细胞活化和促炎细胞因子产生的抑制作用来促进全身炎症反应。MIF可能通过诱导蛋白酶来介导组织破坏。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MIF 抗体 (D-2) 参考文献:

  1. 类风湿关节滑膜组织中巨噬细胞迁移抑制因子的高表达。  |  Onodera, S., et al. 1999. Cytokine. 11: 163-7. PMID: 10089139
  2. 巨噬细胞迁移抑制因子的 Pro-1 在苯基丙酮酸同工酶活性中起催化基的作用。  |  Lubetsky, JB., et al. 1999. Biochemistry. 38: 7346-54. PMID: 10353846
  3. 中和巨噬细胞迁移抑制因子可防止脓毒性休克。  |  Calandra, T., et al. 2000. Nat Med. 6: 164-70. PMID: 10655104
  4. 巨噬细胞迁移抑制因子（MIF）是睾丸体细胞相互作用的旁分泌介质。  |  Meinhardt, A., et al. 2000. Andrologia. 32: 46-8. PMID: 10702866
  5. 促炎介质巨噬细胞迁移抑制因子诱导肌肉中的葡萄糖分解。  |  Benigni, F., et al. 2000. J Clin Invest. 106: 1291-300. PMID: 11086030
  6. 巨噬细胞迁移抑制因子（MIF）可调节由内毒素和革兰氏阴性细菌诱导的先天性免疫反应。  |  Roger, T., et al. 2001. J Endotoxin Res. 7: 456-60. PMID: 11753217
  7. 编码人巨噬细胞迁移抑制因子 cDNA 的分子克隆。  |  Weiser, WY., et al. 1989. Proc Natl Acad Sci U S A. 86: 7522-6. PMID: 2552447
  8. 巨噬细胞迁移抑制因子（MIF）是睾丸体细胞相互作用的旁分泌介质  |  Ruiz-Rosado, Jde D., et al. 2016. Mediators Inflamm. 2016: 9101762. PMID: 27057101
  9. 巨噬细胞迁移抑制因子（MIF）可促进吞噬性中性粒细胞产生次氯酸。  |  Schindler, L., et al. 2021. Redox Biol. 41: 101946. PMID: 33823474
  10. MIF的N端调节参与CD74激活的残基的动态分布。  |  Parkins, A., et al. 2021. Biophys J. 120: 3893-3900. PMID: 34437846
  11. 小鼠和人类巨噬细胞迁移抑制因子（MIF）的纯化, 生物活性和二级结构分析。  |  Bernhagen, J., et al. 1994. Biochemistry. 33: 14144-55. PMID: 7947826
  12. 克隆巨噬细胞迁移抑制因子（MIF）的人类基因。  |  Paralkar, V. and Wistow, G. 1994. Genomics. 19: 48-51. PMID: 8188240

订购信息

产品名称产品编号规格价格数量收藏夹

MIF 抗体 (D-2)

sc-271631
200 µg/ml
$316.00

MIF 抗体 (D-2) AC

sc-271631 AC
500 µg/ml, 25% agarose
$416.00

MIF 抗体 (D-2) Alexa Fluor® 488

sc-271631 AF488
200 µg/ml
$357.00

MIF 抗体 (D-2) Alexa Fluor® 647

sc-271631 AF647
200 µg/ml
$357.00

MIF 抗体 (D-2) FITC

sc-271631 FITC
200 µg/ml
$330.00

MIF 抗体 (D-2) HRP

sc-271631 HRP
200 µg/ml
$316.00

MIF (D-2) 中和勝肽

sc-271631 P
100 µg/0.5 ml
$68.00

MIF 抗体 (D-2) PE

sc-271631 PE
200 µg/ml
$343.00

Is this antibody suitable for neutralization experiments in mice?

Asked by: Al2995
Thank you for your question. We have not tested the antibody for neutralization experiments and, therefore, we are uncertain if it will work in that application.
Answered by: Santa Cruz TS
Date published: 2021-07-10

What application is the blocking peptide sc-271631 P appropriate for?

Asked by: DefinitelyNotMatt
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_8, d_29, h_8CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_271631, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 126ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Good antibody!Works well enough! I got strong bands at 1:500 dilution using 40ug of protein
Date published: 2018-11-08
Rated 5 out of 5 by from Excellent For WBIn MCF7 and T47D cells, diluted at 1:1000, using tricine SDS PAGE, a very specific band was abserved. Excellent antibody.
Date published: 2018-04-12
Rated 1 out of 5 by from DId not detect mouse MIF (HT22 cells)Unfortunately, the antibody did not detect mouse MIF (HT22 & MEF cells) or rat MIF (primary rat cortex tissue) but human MIF (HEK293T cells).
Date published: 2018-01-08
Rated 5 out of 5 by from WB detectionWe usually used this antibody for WB detection MIF protein,we got the specific stainingat dilution 1:250,it's shows good quality.
Date published: 2017-09-03
Rated 4 out of 5 by from Work in IHCGood staining with low background using IHC in mouse tissue
Date published: 2017-08-28
Rated 5 out of 5 by from Good for WB1:500 diluted for wb of rat protein which extracted from h9c2,the band is strong ,but there exist another band below the main band.
Date published: 2017-08-08
Rated 5 out of 5 by from Strong and reliable for IHC and WBExcellent choice if you need a dependable MIF antibody for immunohistochemistry or Western blot. Smart choice!
Date published: 2017-02-06
Rated 4 out of 5 by from Decent band in ARPE-19 cell lysatesDecent band in ARPE-19 cell lysates, correct molecular weight. Couple higher molecular weight non-specific bands.
Date published: 2016-10-05
  • y_2025, m_8, d_29, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_11
  • loc_zh_CN, sid_271631, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 8ms
  • REVIEWS, PRODUCT
MIF 抗体 (D-2) is rated 4.4 out of 5 by 11.
  • y_2025, m_8, d_29, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_11
  • loc_zh_CN, sid_271631, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 111ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。