Date published: 2025-9-6

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MICA/B 抗体 (F-6): sc-137242

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说明书
  • MICA/B 抗体 F-6 是小鼠单克隆 IgG1 κ,MICA/B抗体, 在8篇文献中引用,规格为200 µg/ml
  • 针对氨基酸1-300,在MICA的human起源的N-terminus处进行映射
  • MICA/B 抗体 (F-6) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 MICA and MICB
  • 抗MICA/B抗体(F-6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MICA/B (F-6): sc-137242免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 MICA/B 抗体 (F-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MICA/B 抗体 (F-6) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

MICA/B抗体(F-6)是一种IgG1 κ小鼠单克隆MICA/B抗体(也被称为MICA抗体、MICB抗体、MHC I类多肽相关序列A抗体、MIC-A抗体、主要组织相容性复合体I类链相关蛋白A抗体、MHC I类相关序列A抗体、MHC I类多肽相关序列B抗体、MIC-B抗体、主要组织相容性复合体I类链相关蛋白B抗体、MHC I类相关序列B抗体或HLA I类抗原抗体),它通过WB、IP、IF、IHC(P)和ELISA检测方法识别人类来源的MICA/B蛋白。MICA/B抗体(F-6)既可以作为非偶联形式的抗MICA/B抗体使用,也可以作为多种偶联形式的抗MICA/B抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。MICA和MICB是与主要组织相容性复合体(MHC)I类分子相关的应激诱导抗原。MICA和MICB在上皮性肿瘤中经常表达。这些高度糖基化的细胞表面蛋白在没有常规I类肽配体或与β-2-微球蛋白结合的情况下稳定表达。其表达在增殖或热休克应激的上皮细胞中被诱导。MICA和MICB被表达可变TCR的肠上皮Vδ1 γδ T细胞广泛识别,这表明这些抗原可能在向肠上皮引发免疫反应的细胞应激信号中起核心作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MICA/B 抗体 (F-6) 参考文献:

  1. MICA 和 MICB 的广泛肿瘤缔合作用表达和肿瘤衍生物γ δ T 细胞的识别。  |  Groh, V., et al. 1999. Proc Natl Acad Sci U S A. 96: 6879-84. PMID: 10359807
  2. MICA而不是MICB、TNFA或HLA-DRB1与银屑病关节炎的易感性有关。  |  González, S., et al. 2002. J Rheumatol. 29: 973-8. PMID: 12022360
  3. 抗体介导的MICA和MICB脱落抑制可促进NK细胞驱动的肿瘤免疫。  |  Ferrari de Andrade, L., et al. 2018. Science. 359: 1537-1542. PMID: 29599246
  4. 人类结直肠肿瘤球与免疫细胞的共培养揭示了MICA/B和NKG2A靶向治疗癌症的潜力。  |  Courau, T., et al. 2019. J Immunother Cancer. 7: 74. PMID: 30871626
  5. CD64+ 炎症性 2 型树突状细胞对自然杀伤细胞的 MICa/b 依赖性激活有助于自身免疫。  |  Sánchez-Cerrillo, I., et al. 2023. EMBO J. 42: e113714. PMID: 37916875
  6. 抑制MICA和MICB的脱落可增强NK细胞介导的、对多发性骨髓瘤的类似记忆的细胞毒性。  |  Tahri, S., et al. 2024. Blood Adv. 8: 5365-5370. PMID: 39106432
  7. 哺乳动物主要组织相容性复合体 I 类基因的第二系。  |  Bahram, S., et al. 1994. Proc Natl Acad Sci U S A. 91: 6259-63. PMID: 8022771
  8. 人类 MHC I 类 MICA 基因的核苷酸序列。  |  Bahram, S., et al. 1996. Immunogenetics. 44: 80-1. PMID: 8613147
  9. 胃肠上皮细胞中表达的受细胞压力调控的人类主要组织相容性复合体 I 类基因。  |  Groh, V., et al. 1996. Proc Natl Acad Sci U S A. 93: 12445-50. PMID: 8901601
  10. 人类 MHC I 类 MICB 基因的基因组结构。  |  Bahram, S., et al. 1996. Immunogenetics. 45: 161-2. PMID: 8952966
  11. 肠上皮 gammadelta T 细胞识别压力诱导的 MHC 分子。  |  Groh, V., et al. 1998. Science. 279: 1737-40. PMID: 9497295
  12. 主要组织相容性复合体 I 类相关分子 MIC 家族中与进化相距甚远的成员的多样化, 表达和γ-δT 细胞识别。  |  Steinle, A., et al. 1998. Proc Natl Acad Sci U S A. 95: 12510-5. PMID: 9770516

订购信息

产品名称产品编号规格价格数量收藏夹

MICA/B 抗体 (F-6)

sc-137242
200 µg/ml
RMB2377.00

MICA/B (F-6): m-IgG Fc BP-HRP 套装

sc-528883
200 µg Ab; 10 µg BP
RMB2662.00

MICA/B (F-6): m-IgGκ BP-HRP 套装

sc-521469
200 µg Ab, 40 µg BP
RMB2662.00

MICA/B (F-6): m-IgG1 BP-HRP 套装

sc-543191
200 µg Ab; 20 µg BP
RMB2662.00

MICA/B 抗体 (F-6) AC

sc-137242 AC
500 µg/ml, 25% agarose
RMB3129.00

MICA/B 抗体 (F-6) HRP

sc-137242 HRP
200 µg/ml
RMB2377.00

MICA/B 抗体 (F-6) FITC

sc-137242 FITC
200 µg/ml
RMB2482.00

MICA/B 抗体 (F-6) PE

sc-137242 PE
200 µg/ml
RMB2580.00

MICA/B 抗体 (F-6) Alexa Fluor® 488

sc-137242 AF488
200 µg/ml
RMB2685.00

MICA/B 抗体 (F-6) Alexa Fluor® 546

sc-137242 AF546
200 µg/ml
RMB2685.00

MICA/B 抗体 (F-6) Alexa Fluor® 594

sc-137242 AF594
200 µg/ml
RMB2685.00

MICA/B 抗体 (F-6) Alexa Fluor® 647

sc-137242 AF647
200 µg/ml
RMB2685.00

MICA/B 抗体 (F-6) Alexa Fluor® 680

sc-137242 AF680
200 µg/ml
RMB2685.00

MICA/B 抗体 (F-6) Alexa Fluor® 790

sc-137242 AF790
200 µg/ml
RMB2685.00

Can this MICA/B (F-6) antibody be used in flow cytometry analysis? If not, why?

Asked by: LDozal
Thank you for your question. MICA/B Antibody (F-6): sc-137242 is conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for flow cytometry.
Answered by: Tech Support Europe
Date published: 2020-11-23

Can this MICA/B (F-6): sc-137242 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. We recommend following our recommended protocol, provided below, https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining Alternatively, we do offer this antibody directly conjugated to HRP and a variety of fluorochromes, if you wish to omit using any secondary detection reagent and potential non-specific binding.
Answered by: Technical Support
Date published: 2017-01-30
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Rated 3 out of 5 by from fairly wellfairly well. It has some no-specific bands between main band.
Date published: 2018-02-06
Rated 5 out of 5 by from Produced publishable IHC data on human tissuereference in the primary literature for IHC on human extrahepatic cholangiocarcinoma tissues
Date published: 2017-01-11
Rated 5 out of 5 by from Strong band in WB in MCF7 whole cell lysatesStrong band in WB in MCF7 whole cell lysates. -SCBT QC
Date published: 2015-06-21
Rated 5 out of 5 by from Robust signal in WB in human transfectedRobust signal in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-06-07
Rated 5 out of 5 by from WB publishable data with human HaCaTWB publishable data with human HaCaT, C33-A, SiHa and HeLa cells - SCBT Publication Review
Date published: 2015-02-16
Rated 5 out of 5 by from Beautiful immunoperoxidase cytoplasmic stainingBeautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human esophagus tissue. -SCBT QC
Date published: 2014-08-21
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MICA/B 抗体 (F-6) is rated 4.7 out of 5 by 6.
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