Date published: 2025-9-7

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MICA 抗体 (2C10): sc-23870

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说明书
  • MICA 抗体 2C10 是小鼠单克隆 IgG1 κ,MICA抗体, 在22篇文献中引用,规格为200 µg/ml
  • 针对C1R细胞表达MICA mRNA,在RSV.5neo中稳定转染MICA cDNA后
  • MICA 抗体 (2C10) 推荐用于WB, IP 和 FCM,检测human 来源的 MICA
  • 抗MICA抗体(2C10)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MICA (2C10): sc-23870免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是MICA Antibody (2C10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与MICA Antibody (2C10) 打包提供(请参阅下面的订购信息)。

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関連項目

MICA抗体(2C10)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)和流式细胞术(FCM)检测人源MICA蛋白。MICA (2C10) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。MICA是主要组织相容性复合物(MHC)I类家族的一员,在免疫反应中扮演着至关重要的角色,它充当天然杀伤(NK)细胞和某些T细胞的应激诱导配体。MICA的表达在肿瘤免疫学中尤为重要,因为MICA在上皮肿瘤中通常会上调,表明细胞处于困境,并可能触发免疫反应。这种高度糖化的细胞表面蛋白的独特之处在于其表达独立于传统的I类肽配体,且不需要与β-2微球蛋白结合。肠道上皮Vδ1 γδ T细胞识别MICA,这些细胞表达多种T细胞受体,这凸显了MICA在肠道上皮免疫监测和反应中的重要性,尤其是在细胞应激条件下。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MICA 抗体 (2C10) 参考文献:

  1. MICA 和 MICB 的广泛肿瘤缔合作用表达和肿瘤衍生物γ δ T 细胞的识别。  |  Groh, V., et al. 1999. Proc Natl Acad Sci U S A. 96: 6879-84. PMID: 10359807
  2. 肾脏移植中的MICA。  |  Allison, SJ. 2022. Nat Rev Nephrol. 18: 273. PMID: 35338343
  3. MICA是移植中的组织相容性抗原。  |  Li, XC. 2022. Am J Transplant. 22: 2129. PMID: 36039544
  4. 芬兰的MICA和MICB等位基因分类。  |  Koskela, S., et al. 2023. HLA. 102: 52-61. PMID: 36919857
  5. MICA特异性纳米抗体用于MICA+肿瘤的诊断和免疫治疗。  |  Verhaar, ER., et al. 2024. Front Immunol. 15: 1368586. PMID: 38550583
  6. 基于纳米抗体的CAR NK细胞用于MICA+肿瘤的可能免疫治疗。  |  Verhaar, ER., et al. 2024. PNAS Nexus. 3: pgae184. PMID: 38756234
  7. 哺乳动物主要组织相容性复合体 I 类基因的第二系。  |  Bahram, S., et al. 1994. Proc Natl Acad Sci U S A. 91: 6259-63. PMID: 8022771
  8. 人类 MHC I 类 MICA 基因的等全基因库。  |  Fodil, N., et al. 1996. Immunogenetics. 44: 351-7. PMID: 8781120
  9. 胃肠上皮细胞中表达的受细胞压力调控的人类主要组织相容性复合体 I 类基因。  |  Groh, V., et al. 1996. Proc Natl Acad Sci U S A. 93: 12445-50. PMID: 8901601
  10. 人类 MHC I 类 MICB 基因的基因组结构。  |  Bahram, S., et al. 1996. Immunogenetics. 45: 161-2. PMID: 8952966
  11. 肠上皮 gammadelta T 细胞识别压力诱导的 MHC 分子。  |  Groh, V., et al. 1998. Science. 279: 1737-40. PMID: 9497295
  12. 主要组织相容性复合体 I 类相关分子 MIC 家族中与进化相距甚远的成员的多样化, 表达和γ-δT 细胞识别。  |  Steinle, A., et al. 1998. Proc Natl Acad Sci U S A. 95: 12510-5. PMID: 9770516

订购信息

产品名称产品编号规格价格数量收藏夹

MICA 抗体 (2C10)

sc-23870
200 µg/ml
RMB2377.00

MICA (2C10): m-IgG Fc BP-HRP 套装

sc-528374
200 µg Ab; 10 µg BP
RMB2662.00

MICA (2C10): m-IgGκ BP-HRP 套装

sc-520736
200 µg Ab, 40 µg BP
RMB2662.00

MICA (2C10): m-IgG1 BP-HRP 套装

sc-542901
200 µg Ab; 20 µg BP
RMB2662.00

MICA 抗体 (2C10) AC

sc-23870 AC
500 µg/ml, 25% agarose
RMB3129.00

MICA 抗体 (2C10) HRP

sc-23870 HRP
200 µg/ml
RMB2377.00

MICA 抗体 (2C10) FITC

sc-23870 FITC
200 µg/ml
RMB2482.00

MICA 抗体 (2C10) PE

sc-23870 PE
200 µg/ml
RMB2580.00

MICA 抗体 (2C10) Alexa Fluor® 488

sc-23870 AF488
200 µg/ml
RMB2685.00

MICA 抗体 (2C10) Alexa Fluor® 546

sc-23870 AF546
200 µg/ml
RMB2685.00

MICA 抗体 (2C10) Alexa Fluor® 594

sc-23870 AF594
200 µg/ml
RMB2685.00

MICA 抗体 (2C10) Alexa Fluor® 647

sc-23870 AF647
200 µg/ml
RMB2685.00

MICA 抗体 (2C10) Alexa Fluor® 680

sc-23870 AF680
200 µg/ml
RMB2685.00

MICA 抗体 (2C10) Alexa Fluor® 790

sc-23870 AF790
200 µg/ml
RMB2685.00

Where can I look for the specific epitope where this antibody binds to?

Asked by: LDozal
Thank you for your question. So far, no epitope mapping has been performed for MICA Antibody (2C10): sc-23870, so its exact epitope region is currently not known.
Answered by: Tech Support Europe
Date published: 2020-12-16

Can I receive 10ug free trial sample for sc-23870.

Asked by: Zhinan02139
Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody. Please call us toll-free at (800) 457-3801 option 2, or use the live chat on our website.
Answered by: Technical Support
Date published: 2018-11-14

Can MICA (2C10): sc-23870 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, MICA (2C10): sc-23870 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Tested on HFFF by FACSThis antibody was tested on HFFF cells by FACS and gave a very strong signal, even at dilution 1:100
Date published: 2018-11-19
Rated 5 out of 5 by from Popular antibody!Reliable and sensitive, this is a great choice if you are looking for a solid MICA antibody. Highly recommend it for WB, IP and Flow!
Date published: 2017-01-13
Rated 4 out of 5 by from FACS publishable data with human primaryFACS publishable data with human primary hepatocyte, HCC cell lines (HepG2 and PLC/PRF/5), HeLa, U373, and primary endothelial cells - SCBT Publication Review
Date published: 2015-07-15
Rated 3 out of 5 by from Worked ok with transfected lysate by WBWorked ok with transfected lysate by WB. -SCBT QC
Date published: 2015-05-01
Rated 4 out of 5 by from This antibody was used in a publication forThis antibody was used in a publication for WB on human 293T cells - SCBT Publication Review
Date published: 2015-04-24
Rated 4 out of 5 by from Publishable IF data with human BC3Publishable IF data with human BC3, C1R, U373, HeLa, BJAB, Jurkat, U937, HT29, HepG2, Caco-2, and 293T - SCBT Publication Review
Date published: 2015-01-02
Rated 4 out of 5 by from Clear FCM data of MCF7 cellsClear FCM data of MCF7 cells. -SCBT QC
Date published: 2014-03-08
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MICA 抗体 (2C10) is rated 4.1 out of 5 by 7.
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