Date published: 2025-9-7

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Mi2-β 抗体 (F-7): sc-365639

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说明书
  • Mi2-beta 抗体 F-7 是小鼠单克隆 IgG3 (kappa轻链),Mi2-β抗体,规格为200 µg/ml
  • 特异性抗原位于human物种的Mi2-β的C-terminus的氨基酸1887-1909之间
  • 推荐用于 mouse, rat 和 human 来源的Mi2-β WB, IP, IF, IHC(P) 和 ELISA检测; 也和以下物种反应,包括: 和 equine, canine and porcine
  • 关于如何获取Mi2-β (F-7): sc-365639免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG3 BP-HRPm-IgGκ BP-HRP是Mi2-β抗体(F-7)在WB and IHC(P)应用中的首选二次检测试剂。这些试剂现在与Mi2-β 抗体(F-7)捆绑销售(见下文订购信息)。

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    関連項目

    Mi2-β抗体(F-7)是一种IgG3 κ小鼠单克隆Mi2-β抗体(也被称为Mi2-β抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的Mi2-β蛋白。Mi2-β抗体(F-7)为非偶联抗Mi2-β抗体。在完整细胞中,DNA与组蛋白和其他核蛋白紧密结合形成染色质。染色质重塑被认为是转录调控的关键组成部分,而这种重塑的主要来源是由核小体组蛋白的乙酰化引起的。组蛋白氨基末端尾部结构域中赖氨酸残基的乙酰化导致核小体构象发生变构变化,并增加DNA对转录因子的可及性。相反,组蛋白的去乙酰化与转录沉默有关。染色质结构改变可能是由ATP依赖的多蛋白复合物的作用引起的。其中一种复合物是mSin3辅阻遏物复合物,它包含mSin3、组蛋白去乙酰化酶HDAC1和HDAC2、相关蛋白SAP 30和SAP 18,以及自身抗原Mi2-α和Mi2-β。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    Mi2-β 抗体 (F-7) 参考文献:

    1. 染色质重塑酶Mi-2beta是CD4表达和T细胞发育所必需的。  |  Williams, CJ., et al. 2004. Immunity. 20: 719-33. PMID: 15189737
    2. Ikaros和染色质重塑酶Mi-2beta之间的拮抗作用决定了沉默基因的活性和Cd4基因的表达。  |  Naito, T., et al. 2007. Immunity. 27: 723-34. PMID: 17980631
    3. 染色质重塑酶Mi-2beta在造血干细胞自我更新和多向分化中的作用。  |  Yoshida, T., et al. 2008. Genes Dev. 22: 1174-89. PMID: 18451107
    4. Mi2beta通过消除ACF建立的DNase I超敏位点来显示染色质酶的特异性。  |  Ishii, H., et al. 2009. J Biol Chem. 284: 7533-41. PMID: 19158090
    5. 皮肌炎特异性的主要 Mi-2 自身抗原是一种参与转录激活的假定螺旋酶。  |  Seelig, HP., et al. 1995. Arthritis Rheum. 38: 1389-99. PMID: 7575689
    6. 乙酰化导致的核小体结构变化  |  Bauer, WR., et al. 1994. J Mol Biol. 236: 685-90. PMID: 8114086
    7. 组蛋白乙酰化在转录因子进入核糖体 DNA 的过程中发挥了积极作用。  |  Lee, DY., et al. 1993. Cell. 72: 73-84. PMID: 8422685
    8. 酵母的转录沉默与核小体乙酰化减少有关。  |  Braunstein, M., et al. 1993. Genes Dev. 7: 592-604. PMID: 8458576
    9. 改变染色质结构的多蛋白复合物的抑制和激活作用。  |  Kingston, RE., et al. 1996. Genes Dev. 10: 905-20. PMID: 8608939
    10. 组蛋白去乙酰化酶和一种新型多肽 SAP18 是人类 Sin3 复合物的组成部分。  |  Zhang, Y., et al. 1997. Cell. 89: 357-64. PMID: 9150135
    11. SAP30是一种在人类和酵母之间保守的新型蛋白质,是组蛋白去乙酰化酶复合物的一个组成部分。  |  Zhang, Y., et al. 1998. Mol Cell. 1: 1021-31. PMID: 9651585
    12. 皮肌炎特异性自身抗原 Mi2 是含有组蛋白去乙酰化酶和核小体重塑活性的复合物的一个组成部分。  |  Zhang, Y., et al. 1998. Cell. 95: 279-89. PMID: 9790534

    订购信息

    产品名称产品编号规格价格数量收藏夹

    Mi2-β 抗体 (F-7)

    sc-365639
    200 µg/ml
    RMB2377.00

    Mi2-β (F-7): m-IgGκ BP-HRP 套装

    sc-522306
    200 µg Ab, 40 µg BP
    RMB2662.00

    Mi2-β (F-7): m-IgG3 BP-HRP 套装

    sc-550343
    200 µg Ab; 40 µg BP
    RMB2662.00

    Mi2-β (F-7) 中和勝肽

    sc-365639 P
    100 µg/0.5 ml
    RMB511.00

    What application is the blocking peptide sc-365639 P appropriate for?

    Asked by: Randy McDonald
    Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
    Answered by: Technical Support
    Date published: 2017-02-28
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    • QUESTIONS, PRODUCT
    Rated 1 out of 5 by from UnreliableWe could not see any band in western blot using whole cell extracts or nuclear extracts of human or mouse cells. The same experimental conditions allowed the detection of Mi2-beta using the discontinued sc-11378 antibody.
    Date published: 2021-05-31
    Rated 2 out of 5 by from no good signal in IFI didn't detected good staining signal in 4%PFA fixed embyronic mouse brain(after antigen retrieval)
    Date published: 2018-03-07
    Rated 5 out of 5 by from goodWestern blot, AsPC-1 cell, loading 20ug, antibody dilution, 1:1000, easy to get band at the right size.
    Date published: 2018-02-04
    Rated 4 out of 5 by from works wellI apply this antibody for IP, and it is very effective.
    Date published: 2017-09-04
    Rated 5 out of 5 by from mi2Beta western blot from sample monoclonal Abmi2Beta monoclonal antibody works great. I will recommend it to anyone
    Date published: 2017-08-30
    Rated 5 out of 5 by from Works well in IPThe sample antibody works well with RNA IP protocol in stem cells.
    Date published: 2017-08-16
    Rated 5 out of 5 by from Exceptional immunofluorescence cytoplasmicExceptional immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
    Date published: 2015-04-12
    Rated 5 out of 5 by from Good Western Blot data of Mi2Good Western Blot data of Mi2- expression in Jurkat and K-562 nuclear extracts. -SCBT QC
    Date published: 2013-01-21
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    Mi2-β 抗体 (F-7) is rated 4.0 out of 5 by 8.
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