Date published: 2025-9-7

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MFG-E8 抗体 (MFG-06): sc-8029

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说明书
  • MFG-E8 抗体 MFG-06 是小鼠单克隆 IgG1 κ,MFG-E8抗体, 在13篇文献中引用,规格为200 µg/ml
  • 针对human乳脂球膜组织/细胞制备
  • MFG-E8 抗体 (MFG-06) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 MFG-E8
  • 抗MFG-E8抗体(MFG-06)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MFG-E8 (MFG-06): sc-8029免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 MFG-E8 抗体 (MFG-06) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MFG-E8 抗体 (MFG-06) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

MFG-E8抗体(MFG-06)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和酶联免疫吸附测定(ELISA)检测人类MFG-E8蛋白。抗MFG-E8抗体(MFG-06)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。MFG-E8蛋白,又称乳粘蛋白或BA46,是一种由387个氨基酸组成的细胞外膜蛋白,在细胞粘附和信号传导中起着至关重要的作用。MFG-E8主要位于乳腺上皮细胞的膜上,在人类母乳中含量丰富,MFG-E8可包裹脂质,并作为分化癌的标志物。MFG-E8是整合素β5和整合素β3的特异性配体,在受精和细胞凋亡过程中促进重要的相互作用。MFG-E8已被证明可与轮状病毒结合,抑制病毒复制,并具有抗病毒感染的保护作用。MFG-E8的过度表达与乳腺癌有关,表明其可能参与肿瘤发生,因此MFG-E8成为癌症研究的重要靶标。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MFG-E8 抗体 (MFG-06) 参考文献:

  1. 外周膜蛋白 MFG-E8 作为与膜泡的复合物分泌。  |  Oshima, K., et al. 2002. Eur J Biochem. 269: 1209-18. PMID: 11856354
  2. 鉴定将凋亡细胞与吞噬细胞联系起来的因子。  |  Hanayama, R., et al. 2002. Nature. 417: 182-7. PMID: 12000961
  3. 乳粘连蛋白缺乏会导致细胞凋亡和小鼠动脉粥样硬化加速。  |  Ait-Oufella, H., et al. 2007. Circulation. 115: 2168-77. PMID: 17420351
  4. p63(TP63) 通过 TA 和 DeltaN 异构体之间的相互作用,引起 MFG-E8/lactadherin/BA46 基因的强烈反式激活。  |  Okuyama, T., et al. 2008. Oncogene. 27: 308-17. PMID: 17637751
  5. 人类系统性红斑狼疮患者血清中的乳脂球 EGF 因子 8。  |  Yamaguchi, H., et al. 2008. J Leukoc Biol. 83: 1300-7. PMID: 18303131
  6. 缺乏 alphavbeta5 整合素受体或其配体 MFG-E8:对视网膜功能的不同影响。  |  Nandrot, EF. and Finnemann, SC. 2008. Ophthalmic Res. 40: 120-3. PMID: 18421224
  7. 乳粘菌素阻止体内血栓形成和止血:与血小板磷脂酰丝氨酸暴露相关。  |  Shi, J., et al. 2008. J Thromb Haemost. 6: 1167-74. PMID: 18485093
  8. 含有MFG-E8的许旺细胞衍生外泌体能改变巨噬细胞/微胶质细胞的极化,从而通过SOCS3/STAT3途径减轻脊髓损伤后的炎症。  |  Ren, J., et al. 2023. Cell Death Dis. 14: 70. PMID: 36717543
  9. 人类星形胶质细胞和小胶质细胞通过MFG-E8在阿尔茨海默氏症中表现出对突触的摄取增强。  |  Tzioras, M., et al. 2023. Cell Rep Med. 4: 101175. PMID: 37652017
  10. 靶向癌相关成纤维细胞分泌的MFGE8可阻断食管鳞状细胞癌的血管生成和转移。  |  Liu, B., et al. 2023. Proc Natl Acad Sci U S A. 120: e2307914120. PMID: 37816055
  11. 人乳乳黏蛋白在预防无症状轮状病毒感染中的作用。  |  Newburg, DS., et al. 1998. Lancet. 351: 1160-4. PMID: 9643686
  12. 母乳和母乳喂养早产儿胃液中的乳脂球糖蛋白  |  Peterson, JA., et al. 1998. Pediatr Res. 44: 499-506. PMID: 9773837

订购信息

产品名称产品编号规格价格数量收藏夹

MFG-E8 抗体 (MFG-06)

sc-8029
200 µg/ml
RMB2377.00

MFG-E8 (MFG-06): m-IgG Fc BP-HRP 套装

sc-528208
200 µg Ab; 10 µg BP
RMB2662.00

MFG-E8 (MFG-06): m-IgGκ BP-HRP 套装

sc-520530
200 µg Ab, 40 µg BP
RMB2662.00

MFG-E8 (MFG-06): m-IgG1 BP-HRP 套装

sc-542817
200 µg Ab; 20 µg BP
RMB2662.00

MFG-E8 抗体 (MFG-06) AC

sc-8029 AC
500 µg/ml, 25% agarose
RMB3129.00

MFG-E8 抗体 (MFG-06) HRP

sc-8029 HRP
200 µg/ml
RMB2377.00

MFG-E8 抗体 (MFG-06) FITC

sc-8029 FITC
200 µg/ml
RMB2482.00

MFG-E8 抗体 (MFG-06) PE

sc-8029 PE
200 µg/ml
RMB2580.00

MFG-E8 抗体 (MFG-06) Alexa Fluor® 488

sc-8029 AF488
200 µg/ml
RMB2685.00

MFG-E8 抗体 (MFG-06) Alexa Fluor® 546

sc-8029 AF546
200 µg/ml
RMB2685.00

MFG-E8 抗体 (MFG-06) Alexa Fluor® 594

sc-8029 AF594
200 µg/ml
RMB2685.00

MFG-E8 抗体 (MFG-06) Alexa Fluor® 647

sc-8029 AF647
200 µg/ml
RMB2685.00

MFG-E8 抗体 (MFG-06) Alexa Fluor® 680

sc-8029 AF680
200 µg/ml
RMB2685.00

MFG-E8 抗体 (MFG-06) Alexa Fluor® 790

sc-8029 AF790
200 µg/ml
RMB2685.00

Does this antibody cross react/detect mouse mfge8 protein?

Asked by: x1x1x1
Thank you for your question. This antibody has only been validated for human. There is a 52% homology in mouse . We would not recommend this antibody for mouse samples .
Answered by: BlakeJ
Date published: 2023-05-31

Does sc8029 have neutralizing or blocking activity?

Asked by: Gordon
We do not support this application.
Answered by: Dallas Tech Service
Date published: 2025-04-04

Hello, I'm interested in MFG-E8 antibody (raw and HRP conjugated). However I can't realize which is the catalog number for the HRP conjugated. Thank you Regards

Asked by: Triana
The catalog number for the HRP conjugated antibody is: sc-8029 HRP. The catalog number for the unconjugated antibody is simply: sc-8029.
Answered by: Technical Support
Date published: 2019-02-10

Which epitope this antibody(sc-8029) detects?

Asked by: hhhhj
Thank you for your question. This mouse monoclonal antibody, MFG-E8 (MFG-06): sc-8029, is raised against human milk fat globule membrane tissue / cell preparation. The exact epitope recognized by this antibody has not yet been determined.
Answered by: Technical Support
Date published: 2022-01-20

What is the recommended positive control for MFG-E8 Antibody (MFG-06): sc-8029?

Asked by: Trav11
Thank you for your question. For MFG-E8 Antibody (MFG-06): sc-8029, we have several recommended positive controls, which include: HeLa Whole Cell Lysate (sc-2200), MCF7 Whole Cell Lysate (sc-2206), and MIA PaCa-2 Cell Lysate (sc-2285). If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Technical Support 7
Date published: 2017-01-24
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Rated 5 out of 5 by from Good for WBUseful for Western blot analysis of human cell lysate and secretome.
Date published: 2020-09-04
Rated 5 out of 5 by from Effect is obviousStrong band in WB in A-431 whole cell lysates, very clean background
Date published: 2017-05-11
Rated 5 out of 5 by from ExcellenThis antibody worked very well for WB, we usually use the dilution 1:500
Date published: 2017-04-17
Rated 5 out of 5 by from OutstandingJust a great product for multiple applications includind WB, IF and IHC
Date published: 2017-02-05
Rated 5 out of 5 by from Excellent choice for MFG-E8!Strong and dependable, this antibody will definitely not disappoint in your quest to detect MFG-E8.
Date published: 2017-01-11
Rated 5 out of 5 by from Positive signal in WB in MIA PaCaPositive signal in WB in MIA PaCa-2, MCF7 and A549 whole cell lysates. -SCBT QC
Date published: 2015-06-13
Rated 5 out of 5 by from Robust signal in WB in MCF7 whole cell lysateRobust signal in WB in MCF7 whole cell lysate. -SCBT QC
Date published: 2015-04-06
Rated 5 out of 5 by from Beautiful immunofluorescence membrane andBeautiful immunofluorescence membrane and cytoplasmic staining in methanol-fixed ZR-75-1 cells. -SCBT QC
Date published: 2015-02-06
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MFG-E8 抗体 (MFG-06) is rated 5.0 out of 5 by 8.
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