Date published: 2025-9-6

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Melan-A 抗体 (A103): sc-20032

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说明书
  • Melan-A 抗体 A103 是小鼠单克隆 IgG1 κ,Melan-A抗体, 在42篇文献中引用,规格为200 µg/ml
  • 免疫human物种的重组Melan-A
  • Melan-A 抗体 (A103) 推荐用于 WB, IP, IF, IHC(P) 和 FCM,检测mouse, rat 和human 来源的 Melan-A
  • 抗Melan-A抗体(A103)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Melan-A (A103): sc-20032免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 Melan-A 抗体 (A103) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Melan-A 抗体 (A103) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品,请参阅我们的小鼠 IgG 结合蛋白完整列表。

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関連項目

Melan-A 抗体(A103)是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和流式细胞术(FCM)检测小鼠、大鼠和人源的 Melan-A 蛋白。抗黑色素A抗体(A103)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。Melan-A,也称为MART-1,是一种黑色素细胞分化抗原,在针对黑素瘤的免疫反应中起着至关重要的作用,因为这种蛋白质在黑素瘤、黑色素细胞系和视网膜组织中具有特异性表达。Melan-A的表达至关重要,因为这种蛋白质是细胞毒性T淋巴细胞的目标,而细胞毒性T淋巴细胞对于识别和消除癌细胞至关重要。值得注意的是,Melan-A肽被黑色素瘤患者体内大多数HLA-A2限制性肿瘤浸润淋巴细胞识别,这凸显了Melan-A作为抗原特异性免疫疗法的候选物的潜力。Melan-A肽能够产生抗黑色素瘤细胞毒性T淋巴细胞,这凸显了Melan-A在开发靶向癌症疗法中的重要性,使Melan-A单克隆抗体(A103)成为癌症免疫学领域研究人员和临床医生的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Melan-A 抗体 (A103) 参考文献:

  1. Melan-A/MART-1 特异性 CD8 T 细胞:从胸腺到肿瘤。  |  Pittet, MJ., et al. 2002. Trends Immunol. 23: 325-8. PMID: 12103339
  2. 对黑色素瘤患者进行肿瘤反应性 Melan-A 特异性 CTL 克隆的采纳性转移后,其他 Melan-A 特异性 T 细胞的频率也会增加。  |  Vignard, V., et al. 2005. J Immunol. 175: 4797-805. PMID: 16177129
  3. 同源黑色素-A肽免疫对抗自身黑色素-A/HLA-A2 T细胞交叉反应的影响  |  Colombetti, S., et al. 2006. J Immunol. 176: 6560-7. PMID: 16709813
  4. 接种 Melan-A 肽后,特异性 CD8+ T 细胞对抗原识别的交叉反应减弱。  |  Appay, V., et al. 2006. Eur J Immunol. 36: 1805-14. PMID: 16761313
  5. 解密人类不寻常的 HLA-A2/Melan-A/MART-1 特异性 TCR 反应谱系。  |  Romero, P., et al. 2014. Eur J Immunol. 44: 2567-70. PMID: 25154881
  6. 脑蛋白诱导毛发再色素沉着与 MART-1/Melan-A 重新激活有关。  |  Villarreal-Reyna, G., et al. 2022. Eur J Med Res. 27: 257. PMID: 36411485
  7. 对人类黑色素瘤中均一表达的黑色素细胞特异性蛋白 Melan-A(MART-1)进行血清学分析。  |  Chen, YT., et al. 1996. Proc Natl Acad Sci U S A. 93: 5915-9. PMID: 8650193
  8. 生产重组 MART-1 蛋白和特异性抗 MART-1 多克隆抗体和单克隆抗体:用于表征人类黑色素瘤抗原 MART-1。  |  Kawakami, Y., et al. 1997. J Immunol Methods. 202: 13-25. PMID: 9075767
  9. T 淋巴细胞识别的肿瘤抗原。  |  Van den Eynde, BJ. and Boon, T. 1997. Int J Clin Lab Res. 27: 81-6. PMID: 9266277
  10. 提取自自身蛋白的多肽作为对黑色素瘤/黑色素细胞表位 MART1(27-35) 有反应的人类 CD8+ T 细胞克隆的部分激动剂和拮抗剂。  |  Loftus, DJ., et al. 1998. Cancer Res. 58: 2433-9. PMID: 9622085
  11. 良性黑素细胞痣和原发性皮肤恶性黑素瘤中黑色素-A(MART1)的表达。  |  Busam, KJ., et al. 1998. Am J Surg Pathol. 22: 976-82. PMID: 9706977
  12. 细胞毒性 T 淋巴细胞识别的黑色素瘤相关抗原。  |  Kirkin, AF., et al. 1998. APMIS. 106: 665-79. PMID: 9740504

订购信息

产品名称产品编号规格价格数量收藏夹

Melan-A 抗体 (A103)

sc-20032
200 µg/ml
RMB2377.00

Melan-A (A103): m-IgGκ BP-HRP 套装

sc-520709
200 µg Ab, 40 µg BP
RMB2662.00

Melan-A 抗体 (A103) AC

sc-20032 AC
500 µg/ml, 25% agarose
RMB3129.00

Melan-A 抗体 (A103) HRP

sc-20032 HRP
200 µg/ml
RMB2377.00

Melan-A 抗体 (A103) FITC

sc-20032 FITC
200 µg/ml
RMB2482.00

Melan-A 抗体 (A103) PE

sc-20032 PE
200 µg/ml
RMB2580.00

Melan-A 抗体 (A103) Alexa Fluor® 488

sc-20032 AF488
200 µg/ml
RMB2685.00

Melan-A 抗体 (A103) Alexa Fluor® 546

sc-20032 AF546
200 µg/ml
RMB2685.00

Melan-A 抗体 (A103) Alexa Fluor® 594

sc-20032 AF594
200 µg/ml
RMB2685.00

Melan-A 抗体 (A103) Alexa Fluor® 647

sc-20032 AF647
200 µg/ml
RMB2685.00

Melan-A 抗体 (A103) Alexa Fluor® 680

sc-20032 AF680
200 µg/ml
RMB2685.00

Melan-A 抗体 (A103) Alexa Fluor® 790

sc-20032 AF790
200 µg/ml
RMB2685.00

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Asked by: KIMSEOJ
산타크루즈바이오테크놀러지에 문의 주셔서 감사합니다. 샘플을 받으려면 우리 회사 한국에 있는 distributor에게 물어보세요. 아래는 연락처입니다. 이메일) [email protected] Website) http://korambiotech.com  이메일) [email protected] Website) http://www.essencemedical.com 다른 문제 있으시면 전화:86 21-60936350 이메일: [email protected] 또는 온라인문의로 문의하시면 됩니다. Thank you for your question. For order question ,please contact our local distributor in Korea . Email: [email protected] Website: http://www.essencemedical.com Email: [email protected] Website: http://www.korambiotech.com
Answered by: Technical Service 11
Date published: 2018-12-27

Is the epitope(s) recognized by this antibody extracellular or cytoplasmic?

Asked by: james2010
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody, Melan-A (A103): sc-20032, has not yet been determined. However, we do observe a cytoplasmic staining pattern when using this antibody for immunohistochemistry on formalin-fixed paraffin-embedded tissue sections.
Answered by: Technical Support
Date published: 2017-08-01

Which negative control should be used when using the FITC conjugated Melan-A (A103): sc-20032 monoclonal antibody in flow cytometry?

Asked by: jenni
Thank you for your inquiry. We recommend using normal mouse IgG1 conjugated to FITC, sc-2855
Answered by: Technical Service 11
Date published: 2017-01-10
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Rated 4 out of 5 by from Very specific antibodyWorks very well on frozen tissue section. Specific staining
Date published: 2018-06-07
Rated 5 out of 5 by from Great signal. Human specificProduces a very strong band of correct size by western in melanoma cell line SKmel30 extracts, but not in non-melanoma 293T extracts. This band was not detectable in mouse melanoma cell line B16F10 extracts.
Date published: 2018-02-03
Rated 5 out of 5 by from Superb western and IHC data !!Great product for western and IHC applications; much better antibody than discontinued polyclonals
Date published: 2017-01-14
Rated 2 out of 5 by from Wieder nicht spezifischViele Banden im WB trotz 5% Milch und 3h Waschen. Die Melan A Bande ist zu identifizieren, aber leider nicht spezifisch. Auch normale 293T und ML2 Zellen zeigen Melan A Banden. Dadurch leider für mich nicht nützlich. Das ist nun der zweite Antikörper der nicht spezifisch bindet. Schade.
Date published: 2016-12-29
Rated 5 out of 5 by from Strong, versatile antibodyThe Melan-A Antibody (A103) is a great antibody that works for an array of applications including Western blot, immunohistochemistry, and flow cytometry, with multiple publication citations.
Date published: 2016-12-28
Rated 5 out of 5 by from Great for flow cytometry and Western BlotsWe use this antibody for western blot of melanoma lysates and intracellular flow cytometry staining and it has worked well.
Date published: 2016-10-27
Rated 4 out of 5 by from Publishable data in FCM using human melanomaPublishable data in FCM using human melanoma tumors -SCBT Publication Review
Date published: 2015-07-20
Rated 4 out of 5 by from Strong band seen in WB using C32Strong band seen in WB using C32, SK-MEL-28 -SCBT QC
Date published: 2015-03-31
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Melan-A 抗体 (A103) is rated 4.3 out of 5 by 12.
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