Date published: 2025-9-6

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MEK-7 抗体 (E-7): sc-25288

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说明书
  • MEK-7 抗体 E-7 是小鼠单克隆 IgG2a κ,MEK-7抗体, 在4篇文献中引用,规格为200 µg/ml
  • 免疫human物种的MEK-7的氨基酸1-160
  • MEK-7 抗体 (E-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 MEK-7
  • 抗MEK-7抗体(E-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MEK-7 (E-7): sc-25288免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRPm-IgGκ BP-HRP 是 MEK-7 抗体 (E-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MEK-7 抗体 (E-7) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

MEK-7抗体(E-7)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的MEK-7蛋白。抗MEK-7抗体(E-7)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。MEK-7,也称为MKK-7,在激活c-Jun N-末端激酶(JNK)信号通路中起着至关重要的作用,该信号通路可调节细胞对压力、炎症和细胞凋亡的反应。MEK-7介导的JNK激活在各种生理和病理过程中尤为重要,包括细胞分化、存活和对环境刺激的响应。了解MEK-7功能和JNK激活可以为癌症和神经退行性疾病等疾病的潜在机制提供宝贵的见解,使MEK-7(E-7)单克隆抗体成为研究人员研究这些关键途径的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MEK-7 抗体 (E-7) 参考文献:

  1. MEK 的主要结构,MEK 是一种蛋白激酶,能使 ERK 基因产物磷酸化。  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
  2. MKK7是JNK信号传导的重要调节因子,参与癌细胞的存活:一种新兴的抗癌治疗靶点。  |  Park, JG., et al. 2019. Ther Adv Med Oncol. 11: 1758835919875574. PMID: 31579105
  3. 成熟神经元中的MKK7缺陷会损害小鼠的亲代行为。  |  Shin, T., et al. 2021. Genes Cells. 26: 5-17. PMID: 33098150
  4. 通过粗纳米级库优化共价MKK7抑制剂。  |  Gehrtz, P., et al. 2022. J Med Chem. 65: 10341-10356. PMID: 35912476
  5. 一种新的人类蛋白激酶信号转导途径的组成部分。  |  Zhou, G., et al. 1995. J Biol Chem. 270: 12665-9. PMID: 7759517
  6. 由 MEK 和 MKK 同工酶定义的独立人类 MAP 激酶信号转导途径。  |  Dérijard, B., et al. 1995. Science. 267: 682-5. PMID: 7839144
  7. 一种新的哺乳动物丝裂原活化蛋白激酶激酶 MKK2 的鉴定和表征。  |  Wu, J., et al. 1993. Mol Cell Biol. 13: 4539-48. PMID: 8393135
  8. 新型 MAP 激酶激酶 (MKK6) 的结构和功能特性。  |  Han, J., et al. 1996. J Biol Chem. 271: 2886-91. PMID: 8621675
  9. 一种新的丝裂原活化蛋白激酶(p38beta)的结构和功能特征。  |  Jiang, Y., et al. 1996. J Biol Chem. 271: 17920-6. PMID: 8663524
  10. 丝裂原活化蛋白激酶激酶 7 是 c-Jun NH2 端激酶的激活剂。  |  Tournier, C., et al. 1997. Proc Natl Acad Sci U S A. 94: 7337-42. PMID: 9207092
  11. MKK7 是一种应激激活的丝裂原活化蛋白激酶激酶,在功能上与半蝶呤有关。  |  Holland, PM., et al. 1997. J Biol Chem. 272: 24994-8. PMID: 9312105
  12. 人 JNKK2(一种新型 Jun NH2-末端激酶特异性激酶)的分子克隆和特征描述。  |  Wu, Z., et al. 1997. Mol Cell Biol. 17: 7407-16. PMID: 9372971

订购信息

产品名称产品编号规格价格数量收藏夹

MEK-7 抗体 (E-7)

sc-25288
200 µg/ml
RMB2377.00

MEK-7 (E-7): m-IgG Fc BP-HRP 套装

sc-528396
200 µg Ab; 10 µg BP
RMB2662.00

MEK-7 (E-7): m-IgGκ BP-HRP 套装

sc-520767
200 µg Ab, 40 µg BP
RMB2662.00

MEK-7 (E-7): m-IgG2a BP-HRP 套装

sc-547084
200 µg Ab; 10 µg BP
RMB2662.00

MEK-7 抗体 (E-7) AC

sc-25288 AC
500 µg/ml, 25% agarose
RMB3129.00

MEK-7 抗体 (E-7) HRP

sc-25288 HRP
200 µg/ml
RMB2377.00

MEK-7 抗体 (E-7) FITC

sc-25288 FITC
200 µg/ml
RMB2482.00

MEK-7 抗体 (E-7) PE

sc-25288 PE
200 µg/ml
RMB2580.00

MEK-7 抗体 (E-7) Alexa Fluor® 488

sc-25288 AF488
200 µg/ml
RMB2685.00

MEK-7 抗体 (E-7) Alexa Fluor® 546

sc-25288 AF546
200 µg/ml
RMB2685.00

MEK-7 抗体 (E-7) Alexa Fluor® 594

sc-25288 AF594
200 µg/ml
RMB2685.00

MEK-7 抗体 (E-7) Alexa Fluor® 647

sc-25288 AF647
200 µg/ml
RMB2685.00

MEK-7 抗体 (E-7) Alexa Fluor® 680

sc-25288 AF680
200 µg/ml
RMB2685.00

MEK-7 抗体 (E-7) Alexa Fluor® 790

sc-25288 AF790
200 µg/ml
RMB2685.00

I am using MEK-7 (E-7): sc-25288 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: cjMara
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Very good in WB!!!Very good signal for my HCT116, BT474 and KYSE150 cells!!!! 15 ug lysate should be used at least...
Date published: 2018-02-01
Rated 5 out of 5 by from Excelente en inmunoblotUn anticuerpo que da unos resultados muy robustos, claros y altamente específicos. Lo hemos probado en el análisis por inmunoblot de extractos crudos de macrófagos de médula ósea. El primer análisis lo diluímos 1:250 pero seguro que funciona más diluído.
Date published: 2017-09-13
Rated 5 out of 5 by from very good western blot bandsWe used this antibody for MEK7 expression detection with western blot. We got very nice bands at the correct molecular weight for HeLa cells
Date published: 2017-02-01
Rated 4 out of 5 by from Very good antibody for Western blotting.This product has been independently tested and validated in-house at Kinexus Bioinformatics Corporation as a potent and highly specific antibody for Western blotting with a mixture of mouse brain, testes and thymus tissue lysates. It detects an appropriately sized ~47 kDa target protein in immunoblots with medium to strong intensity.
Date published: 2016-10-13
Rated 5 out of 5 by from Very strong bands produced when using thisVery strong bands produced when using this antibody against A-431, HeLa, C32, NIH/3T3 and KNRK whole cell lysates. -SCBT QC
Date published: 2015-05-19
Rated 5 out of 5 by from Favorable bands achieved in nonFavorable bands achieved in non-transfected and human MEK-7 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-01-25
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic andExcellent immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human placenta tissue. -SCBT QC
Date published: 2015-01-07
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MEK-7 抗体 (E-7) is rated 4.9 out of 5 by 7.
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