Date published: 2025-9-6

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MEK-4 抗体 (G-7): sc-376838

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说明书
  • MEK-4 抗体 G-7 是小鼠单克隆 IgG1 κ,MEK-4抗体, 在3篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于mouse物种的MEK-4的N-terminus附近的氨基酸43-79之间
  • MEK-4 抗体 (G-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 MEK-4; 也和以下物种反应,包括: canine
  • 抗MEK-4抗体(G-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MEK-4 (G-7): sc-376838免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成MEK-4 抗体 (G-7)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

MEK-4抗体(G-7)是一种IgG1 κ小鼠单克隆MEK-4抗体(也称为MKK4抗体或MEKKK 4抗体),可通过WB、IP、IF、IHC(P)和ELISA检测来自小鼠、大鼠和人类的MEK-4蛋白。MEK-4抗体(G-7)既可以是非偶联的抗MEK-4抗体形式,也可以是多种偶联形式的抗MEK-4抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。已确定位于MAP激酶上游并负责其激活的一组蛋白激酶。该家族的原型成员,称为MAP激酶激酶或MEK-1,特异性磷酸化ERK的Thr-Glu-Tyr基序中存在的MAP激酶调节性苏氨酸和酪氨酸残基。第二个MEK家族成员MEK-2在底物特异性上与MEK-1相似。MEK-3(或MKK-3)激活p38 MAP激酶,而MEK-4(也称为SEK1或MKK-4)激活p38和JNK MAP激酶。MEK-5似乎特异性磷酸化ERK5,而MEK-6磷酸化p38和p38b。MEK-7(或MKK-7)磷酸化并激活JNK信号转导通路。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MEK-4 抗体 (G-7) 参考文献:

  1. 血管活性肽和垂体腺苷酸环化酶激活多肽可抑制内毒素激活的小胶质细胞中的 MEKK1/MEK4/JNK 信号通路。  |  Delgado, M. 2002. Biochem Biophys Res Commun. 293: 771-6. PMID: 12054537
  2. MEK 的主要结构,MEK 是一种蛋白激酶,能使 ERK 基因产物磷酸化。  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
  3. ELF-1的鉴定和克隆,ELF-1是Mek4和Sek受体酪氨酸激酶的发育表达配体。  |  Cheng, HJ. and Flanagan, JG. 1994. Cell. 79: 157-68. PMID: 7522971
  4. 一种新的人类蛋白激酶信号转导途径的组成部分。  |  Zhou, G., et al. 1995. J Biol Chem. 270: 12665-9. PMID: 7759517
  5. 由 MEK 和 MKK 同工酶定义的独立人类 MAP 激酶信号转导途径。  |  Dérijard, B., et al. 1995. Science. 267: 682-5. PMID: 7839144
  6. 一种新的哺乳动物丝裂原活化蛋白激酶激酶 MKK2 的鉴定和表征。  |  Wu, J., et al. 1993. Mol Cell Biol. 13: 4539-48. PMID: 8393135
  7. 新型 MAP 激酶激酶 (MKK6) 的结构和功能特性。  |  Han, J., et al. 1996. J Biol Chem. 271: 2886-91. PMID: 8621675
  8. 一种新的丝裂原活化蛋白激酶(p38beta)的结构和功能特征。  |  Jiang, Y., et al. 1996. J Biol Chem. 271: 17920-6. PMID: 8663524
  9. Tyro4/Mek4/Cek4基因的表达特异性地标记了胚胎运动神经元亚群及其肌肉目的基因。  |  Kilpatrick, TJ., et al. 1996. Mol Cell Neurosci. 7: 62-74. PMID: 8812059
  10. 丝裂原活化蛋白激酶激酶 7 是 c-Jun NH2 端激酶的激活剂。  |  Tournier, C., et al. 1997. Proc Natl Acad Sci U S A. 94: 7337-42. PMID: 9207092
  11. MKK7 是一种应激激活的丝裂原活化蛋白激酶激酶,在功能上与半蝶呤有关。  |  Holland, PM., et al. 1997. J Biol Chem. 272: 24994-8. PMID: 9312105
  12. 人 JNKK2(一种新型 Jun NH2-末端激酶特异性激酶)的分子克隆和特征描述。  |  Wu, Z., et al. 1997. Mol Cell Biol. 17: 7407-16. PMID: 9372971

订购信息

产品名称产品编号规格价格数量收藏夹

MEK-4 抗体 (G-7)

sc-376838
200 µg/ml
RMB2377.00

MEK-4 抗体 (G-7) AC

sc-376838 AC
500 µg/ml, 25% agarose
RMB3129.00

MEK-4 抗体 (G-7) HRP

sc-376838 HRP
200 µg/ml
RMB2377.00

MEK-4 抗体 (G-7) FITC

sc-376838 FITC
200 µg/ml
RMB2482.00

MEK-4 抗体 (G-7) PE

sc-376838 PE
200 µg/ml
RMB2580.00

MEK-4 抗体 (G-7) Alexa Fluor® 488

sc-376838 AF488
200 µg/ml
RMB2685.00

MEK-4 抗体 (G-7) Alexa Fluor® 546

sc-376838 AF546
200 µg/ml
RMB2685.00

MEK-4 抗体 (G-7) Alexa Fluor® 594

sc-376838 AF594
200 µg/ml
RMB2685.00

MEK-4 抗体 (G-7) Alexa Fluor® 647

sc-376838 AF647
200 µg/ml
RMB2685.00

MEK-4 抗体 (G-7) Alexa Fluor® 680

sc-376838 AF680
200 µg/ml
RMB2685.00

MEK-4 抗体 (G-7) Alexa Fluor® 790

sc-376838 AF790
200 µg/ml
RMB2685.00

MEK-4 (G-7) 中和勝肽

sc-376838 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-376838 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from work well in western blot at 1:200,ov90 cellActually,it is great.The antibody works vary well.
Date published: 2017-09-20
Rated 5 out of 5 by from Perfect bands with MEK4 (sc-376838)Cos-7 was transfected with pcDNA-3HA, MEK1-HA, MEK2-HA and MEK4-HA using lipo2000, 48h later, harvest cells with RIPA for western blot assay using MEK4(sc-376838), MEK4 overexpression was observed and the antibody not interact with MEK1 and MEK2.
Date published: 2017-04-23
Rated 5 out of 5 by from Great species reactivityConfirmed reactivity with human, mouse and canine samples seen in Western Blot with whole cell lysates.
Date published: 2017-02-07
Rated 4 out of 5 by from Potent and very specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was medium detection of the ~45 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested.
Date published: 2016-11-10
Rated 5 out of 5 by from Produced superb immunoperoxidase apical membraneProduced superb immunoperoxidase apical membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human kidney tissue. -SCBT QC
Date published: 2015-04-19
Rated 5 out of 5 by from Strong bands in western blot analysis of MEKStrong bands in western blot analysis of MEK-4 in NIH/3T3, HeLa, MDCK, Hep G2, K-562 and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-03-25
Rated 5 out of 5 by from WB: singularWB: singular, clean band in Src-3T3 whole cell lysate. -SCBT Publication Review
Date published: 2015-03-18
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MEK-4 抗体 (G-7) is rated 4.9 out of 5 by 7.
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