Date published: 2025-9-6
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MEK-2 Antibody (A-1) - Western Blotting - Image 377270
MEK-2 抗体 (A-1): sc-13159. Jurkat (A), HeLa (B), MCF7 (C), TK-1 (D), c4 (E) 和 BYDP (F) 全细胞裂解液中 MEK-2 表达的 Western 印迹分析.
MEK-2 抗体 (A-1): sc-13159. Jurkat (A), HeLa (B), MCF7 (C), TK-1 (D), c4 (E) 和 BYDP (F) 全细胞裂解液中 MEK-2 表达的 Western 印迹分析.

MEK-2 抗体 (A-1): sc-13159

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说明书
  • MEK-2 抗体 A-1 是小鼠单克隆 IgG2a κ，MEK-2抗体, 在7篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的MEK-2的N-terminus的氨基酸6-25之间
  • MEK-2 抗体 (A-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 MEK-2
  • 抗MEK-2抗体（A-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MEK-2 (A-1): sc-13159的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 MEK-2 抗体 (A-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MEK-2 抗体 (A-1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 MEK 抗体 包括 MEK-1, MEK-2, MEK-3, MEK-4, MEK-5, MEK-6和MEK-7

MEK-2抗体（A-1）是一种鼠单克隆IgG2a kappa轻链抗体，用于检测小鼠、大鼠和人类样本中的促分裂原活化蛋白激酶2（MEK-2），也称为MAP2K2。MEK-2单克隆抗体（A-1）靶向人类MEK-2 N端6-25位氨基酸之间的表位，确保在各种应用中精确可靠的结合，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHC-P）和酶联免疫吸附测定（ELISA）。MEK-2在MAPK/ERK信号传导通路中起着关键作用，它是一种双特异性激酶，可磷酸化并激活ERK1/2，而ERK1/2对于调节细胞分裂、分化及存活至关重要。MEK-2的正常功能对于正常的细胞过程至关重要，其失调与多种癌症和其他疾病有关，因此精确检测对于研究和临床诊断都至关重要。MEK-2单克隆抗体（A-1）有结合和非结合两种形式，包括与琼脂糖、HRP、PE、FITC和多种Alexa Fluor®染料结合的抗体，为不同的实验设置和检测系统提供了多功能性。MEK-2单克隆抗体（A-1）推荐用于研究信号转导、癌症生物学和治疗开发的研究人员。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MEK-2 抗体 (A-1) 参考文献:

  1. MEK 的主要结构，MEK 是一种蛋白激酶，能使 ERK 基因产物磷酸化。  |  Crews, CM., et al. 1992. Science. 258: 478-80. PMID: 1411546
  2. MEK-2是一种秀丽隐杆线虫的MAP激酶激酶，在Ras介导的阴唇诱导和其他发育事件中发挥作用。  |  Wu, Y., et al. 1995. Genes Dev. 9: 742-55. PMID: 7729690
  3. 一种新的人类蛋白激酶信号转导途径的组成部分。  |  Zhou, G., et al. 1995. J Biol Chem. 270: 12665-9. PMID: 7759517
  4. 由 MEK 和 MKK 同工酶定义的独立人类 MAP 激酶信号转导途径。  |  Dérijard, B., et al. 1995. Science. 267: 682-5. PMID: 7839144
  5. 一种新的哺乳动物丝裂原活化蛋白激酶激酶 MKK2 的鉴定和表征。  |  Wu, J., et al. 1993. Mol Cell Biol. 13: 4539-48. PMID: 8393135
  6. 新型 MAP 激酶激酶 (MKK6) 的结构和功能特性。  |  Han, J., et al. 1996. J Biol Chem. 271: 2886-91. PMID: 8621675
  7. 一种新的丝裂原活化蛋白激酶（p38beta）的结构和功能特征。  |  Jiang, Y., et al. 1996. J Biol Chem. 271: 17920-6. PMID: 8663524
  8. 趋化肽诱导激活MEK-2，这是人类中性粒细胞中的主要同工型。由毛地黄素抑制。  |  Downey, GP., et al. 1996. J Biol Chem. 271: 21005-1011. PMID: 8702863
  9. 人类肺癌细胞系中MEK-1和MEK-2基因编码序列的突变分析。  |  Bansal, A., et al. 1997. Oncogene. 14: 1231-4. PMID: 9121773
  10. 丝裂原活化蛋白激酶激酶 7 是 c-Jun NH2 端激酶的激活剂。  |  Tournier, C., et al. 1997. Proc Natl Acad Sci U S A. 94: 7337-42. PMID: 9207092
  11. MKK7 是一种应激激活的丝裂原活化蛋白激酶激酶，在功能上与半蝶呤有关。  |  Holland, PM., et al. 1997. J Biol Chem. 272: 24994-8. PMID: 9312105
  12. 人 JNKK2（一种新型 Jun NH2-末端激酶特异性激酶）的分子克隆和特征描述。  |  Wu, Z., et al. 1997. Mol Cell Biol. 17: 7407-16. PMID: 9372971

订购信息

产品名称产品编号规格价格数量收藏夹

MEK-2 抗体 (A-1)

sc-13159
200 µg/ml
RMB2377.00

MEK-2 (A-1): m-IgG Fc BP-HRP 套装

sc-528278
200 µg Ab; 10 µg BP
RMB2662.00

MEK-2 (A-1): m-IgGκ BP-HRP 套装

sc-520617
200 µg Ab, 40 µg BP
RMB2662.00

MEK-2 (A-1): m-IgG2a BP-HRP 套装

sc-547056
200 µg Ab; 10 µg BP
RMB2662.00

MEK-2 抗体 (A-1) AC

sc-13159 AC
500 µg/ml, 25% agarose
RMB3129.00

MEK-2 抗体 (A-1) HRP

sc-13159 HRP
200 µg/ml
RMB2377.00

MEK-2 抗体 (A-1) FITC

sc-13159 FITC
200 µg/ml
RMB2482.00

MEK-2 抗体 (A-1) PE

sc-13159 PE
200 µg/ml
RMB2580.00

MEK-2 抗体 (A-1) Alexa Fluor® 488

sc-13159 AF488
200 µg/ml
RMB2685.00

MEK-2 抗体 (A-1) Alexa Fluor® 546

sc-13159 AF546
200 µg/ml
RMB2685.00

MEK-2 抗体 (A-1) Alexa Fluor® 594

sc-13159 AF594
200 µg/ml
RMB2685.00

MEK-2 抗体 (A-1) Alexa Fluor® 647

sc-13159 AF647
200 µg/ml
RMB2685.00

MEK-2 抗体 (A-1) Alexa Fluor® 680

sc-13159 AF680
200 µg/ml
RMB2685.00

MEK-2 抗体 (A-1) Alexa Fluor® 790

sc-13159 AF790
200 µg/ml
RMB2685.00

MEK-2 (A-1) 中和勝肽

sc-13159 P
100 µg/0.5 ml
RMB511.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 3
Date published: 2017-02-01
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Rated 4 out of 5 by from Good specificity in WB in HEK293T cellsI have used this Ab for western blot in HEK293T cell lysate under the following condition and I saw nic bands as visible in an attached image. conditon: 9% gel Dilution: 1:700 Incubation: 2h R.T High exposure
Date published: 2021-05-04
Rated 4 out of 5 by from Great signalI bought the ab last month and it gives a good signal.
Date published: 2019-07-01
Rated 4 out of 5 by from good sensitivity for WBNo cross-reactivity with MEK-1. Detect multiple non-specific bands with 293 lysate, but distinguishable from MEK-2. Be careful of a weak non-specific band slightly above MEK-2
Date published: 2019-05-29
Rated 5 out of 5 by from Excellent antibody for westernsdiluted at 1000X and used with Kappa-binding HRP as secondary and the blot turned out perfect.
Date published: 2017-06-16
Rated 5 out of 5 by from Perfect bands with MEK2(sc-13159)Cos-7 was transfected with pcDNA-3HA, MEK1-HA, MEK2-HA and MEK4-HA using lipo2000, 48h later, harvest cells with RIPA for western blot assay using MEK2(sc-13159, 1:10 00 dilution), MEK2 overexpression was observed and the antibody not interact with MEK1 and MEK4.
Date published: 2017-04-23
Rated 5 out of 5 by from Worked great in IP stainWe used this antibody in an IP stain and got clear results for human cells
Date published: 2016-12-20
Rated 5 out of 5 by from HighlyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was very strong detection of the ~47 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested, but the antibody seems to pick up a slightly lower molecular mass protein that is the same size as MEK1.
Date published: 2016-11-10
Rated 5 out of 5 by from Great WB results with the following: JurkatGreat WB results with the following: Jurkat, K-562, A-431, NIH/3T3 and WEHI-231 whole cell lysates. -SCBT QC
Date published: 2015-05-03
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MEK-2 抗体 (A-1) is rated 4.7 out of 5 by 12.
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