Date published: 2025-9-7
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MEF-2A Antibody (B-4) - Western Blotting - Image 359701
MEF-2A 抗体 (B-4): sc-17785. Western印迹分析 Daudi (A), SH-SY5Y (B), NIH/3T3 (C) 和 A-10 (D) 全细胞裂解液中 MEF-2A 的表达. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
MEF-2A 抗体 (B-4): sc-17785. Western印迹分析 Daudi (A), SH-SY5Y (B), NIH/3T3 (C) 和 A-10 (D) 全细胞裂解液中 MEF-2A 的表达. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.

MEF-2A 抗体 (B-4): sc-17785

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说明书
  • MEF-2A 抗体 B-4 是小鼠单克隆 IgG1 κ，MEF-2A抗体, 在36篇文献中引用，规格为200 µg/ml
  • 免疫human来源MEF-2A位于N-terminus 的1-300氨基酸
  • MEF-2A 抗体 (B-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 MEF-2A
  • 抗MEF-2A抗体（B-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-17785 X, 200 µg/0.1 ml)
  • 关于如何获取MEF-2A (B-4): sc-17785的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 MEF-2A 抗体 (B-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MEF-2A 抗体 (B-4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 MEF 抗体 包括 MEF-2, MEF-2A, MEF-2B, MEF-2C和MEF-2D

MEF-2A抗体（B-4）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人源的MEF-2A。抗MEF-2A抗体（B-4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。肌细胞增强因子-2（MEF-2）家族转录因子在调节T细胞、神经细胞和肌肉细胞的发展和功能方面发挥着至关重要的作用，其中MEF-2A、-2B、-2C和-2D四个家族成员由可变剪接转录物产生。这些蛋白质以同源和异源二聚体的形式结合到靶基因启动子区域的MEF-2位点，表明它们在胚胎发育和发育过程中的功能是不同的。值得注意的是，MEF-2A参与将中胚层前体细胞分化成肌细胞的过程，这一过程揭示了肌肉基因表达所需的多种组织特异性因子。这些因子包括肌源性基本螺旋-环-螺旋蛋白，如MyoD、myogenin、Myf-5和MRF4，它们与MEF-2家族一起与肌肉特异性基因调控区域中的特定DNA序列结合，从而协调肌肉发育和功能所需的复杂基因表达模式。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MEF-2A 抗体 (B-4) 参考文献:

  1. 肌细胞增强因子-2对转录共抑制因子Cabin1的序列特异性招募。  |  Han, A., et al. 2003. Nature. 422: 730-4. PMID: 12700764
  2. KIN-29 SIK 通过 MEF2 转录因子和 II 类 HDAC 调节化学感受器基因的表达。  |  van der Linden, AM., et al. 2007. EMBO J. 26: 358-70. PMID: 17170704
  3. p38α/β丝裂原活化蛋白激酶介导 CREB 结合蛋白的招募，以保持肌管中快速肌球蛋白重链 IId/x 基因的活性。  |  Meissner, JD., et al. 2007. J Biol Chem. 282: 7265-75. PMID: 17210568
  4. 编码天冬氨酰-beta-羟化酶, 接合素和接合酸的人类 A beta H-J-J 基因座表达的多级控制。  |  Feriotto, G., et al. 2006. Ann N Y Acad Sci. 1091: 184-90. PMID: 17341613
  5. 翻译后修饰对 SRC 家族辅助激活因子的调控。  |  Li, S. and Shang, Y. 2007. Cell Signal. 19: 1101-12. PMID: 17368849
  6. 乳腺癌缔合作用转录抑制因子 PLU-1/JARID1B 直接与组蛋白去乙酰化酶相互作用。  |  Barrett, A., et al. 2007. Int J Cancer. 121: 265-75. PMID: 17373667
  7. 神经毒素对肌细胞增强因子-2 转录因子的调控。  |  She, H. and Mao, Z. 2011. Neurotoxicology. 32: 563-6. PMID: 21741404
  8. 破骨细胞中肌细胞增强因子 2 的表达缺失会导致相反的骨骼表型。  |  Blixt, N., et al. 2020. Bone. 138: 115466. PMID: 32512162
  9. 肌生成因子肌细胞增强因子-2的磷酸化对体内肌生成的影响  |  Vishal, K., et al. 2023. Mol Cell Biol. 43: 241-253. PMID: 37184381
  10. 在小鼠胚胎癌细胞中差异表达的 MEF2B 同源物。  |  Hidaka, K., et al. 1995. Biochem Biophys Res Commun. 213: 555-60. PMID: 7646512
  11. MADS 域转录增强因子 2 (MEF2) 基因家族四个成员在人类染色体 15q26, 19p12, 5q14 和 1q12-q23 上的区域染色体分配。  |  Hobson, GM., et al. 1995. Genomics. 29: 704-11. PMID: 8575763
  12. p38 对 MEF2 转录因子家族的调控。  |  Zhao, M., et al. 1999. Mol Cell Biol. 19: 21-30. PMID: 9858528

订购信息

产品名称产品编号规格价格数量收藏夹

MEF-2A 抗体 (B-4)

sc-17785
200 µg/ml
RMB2377.00

MEF-2A (B-4): m-IgG Fc BP-HRP 套装

sc-528312
200 µg Ab; 10 µg BP
RMB2662.00

MEF-2A (B-4): m-IgGκ BP-HRP 套装

sc-520656
200 µg Ab, 40 µg BP
RMB2662.00

MEF-2A (B-4): m-IgG1 BP-HRP 套装

sc-542860
200 µg Ab; 20 µg BP
RMB2662.00

MEF-2A 抗体 (B-4) X

sc-17785 X
200 µg/0.1 ml
RMB2377.00

MEF-2A 抗体 (B-4) AC

sc-17785 AC
500 µg/ml, 25% agarose
RMB3129.00

MEF-2A 抗体 (B-4) HRP

sc-17785 HRP
200 µg/ml
RMB2377.00

MEF-2A 抗体 (B-4) FITC

sc-17785 FITC
200 µg/ml
RMB2482.00

MEF-2A 抗体 (B-4) PE

sc-17785 PE
200 µg/ml
RMB2580.00

MEF-2A 抗体 (B-4) Alexa Fluor® 488

sc-17785 AF488
200 µg/ml
RMB2685.00

MEF-2A 抗体 (B-4) Alexa Fluor® 546

sc-17785 AF546
200 µg/ml
RMB2685.00

MEF-2A 抗体 (B-4) Alexa Fluor® 594

sc-17785 AF594
200 µg/ml
RMB2685.00

MEF-2A 抗体 (B-4) Alexa Fluor® 647

sc-17785 AF647
200 µg/ml
RMB2685.00

MEF-2A 抗体 (B-4) Alexa Fluor® 680

sc-17785 AF680
200 µg/ml
RMB2685.00

MEF-2A 抗体 (B-4) Alexa Fluor® 790

sc-17785 AF790
200 µg/ml
RMB2685.00

Hello, professor. We bought MEF-2 Antibody (C-21): sc-313 before, it worked well. However, it has been discontinued and replaced by MEF-2A (B-4): sc-17785, which is not work in our experiments. Would it be possible to obtain MEF-2 Antibody (C-21) again?

Asked by: Haisong
Thank you for your question. This antibody MEF-2 (C-21) has been discontinued as of 2016. We no longer carry this product and would not be able to provide this to you at this time. If you have any further questions, or need help with any troubleshooting, please contact Technical Service.
Answered by: Technical Service 3
Date published: 2022-07-18

Hi, I noticed that one of your references for this product sc-17785 was in skeletal mucle of zebrafih lavea. I wondered if you know of anyone using this in adult zebrafish hearts.

Asked by: Laurenamyb
Thank you for your question. We are not currently aware of any data or product citations specifically using this antibody in adult zebrafish hearts. The only product citation we are aware of in zebrafish is on our website and can be found using PMID: 29518074.
Answered by: Technical Support
Date published: 2022-07-18

I need to use an antibody to detect the protein in samples of bovine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 7
Date published: 2017-02-04

Hello. Would it be possible to obtain a sample of the MEF2A B4 antibody? We want to try using it for immunoprecipitations in mouse osteoclasts.

Asked by: Blixt
Thank you for your question. Please contact our Technical Service Department to request a complimentary 10 µg sample of this mouse monoclonal antibody, MEF-2A (B-4): sc-17785. Please call us directly at (800) 457-3801 option 2, or email [email protected], or use our live chat.
Answered by: Technical Support
Date published: 2017-01-10
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Rated 2 out of 5 by from No the best option that you can buy.The antibody (new dilution) didn't work in re-used membrames. I just could use it with new membranes and new dilutions. I think other brands are more flexible with this points.
Date published: 2019-09-20
Rated 4 out of 5 by from Antibody reviewThis antibody is okay to use for immunoblotting in mouse tissue lysate at 1:500 dilution.
Date published: 2018-06-14
Rated 5 out of 5 by from it worksThe MEF-2A antibody works well.The expression of MEF-2 antibody in human is strong signal.
Date published: 2017-12-14
Rated 4 out of 5 by from Good AbIt's a good Ab, I saw the band I want in IP. Although there are some un-specific band the targeted band is the strongest one.
Date published: 2017-11-26
Rated 5 out of 5 by from Works WellI can get a good result in 1:1000 dilution, it is clear, specific works normally amd stains very brightly in cells.
Date published: 2017-10-27
Rated 5 out of 5 by from Heart sample excellent!I try to use this Ab as IP, it is good for this experiment except for a little high concentration. It is no problem to do western blot!
Date published: 2017-08-01
Rated 5 out of 5 by from ChIP grade MEF2A abThis antibody works very nicely in ChIP applications with human samples
Date published: 2017-04-07
Rated 5 out of 5 by from Very efficient antibody indeed.Satisfactory Western Blot data of Borealin expression in HL-60 whole cell lysate and rat kidney tissue extract ,very efficient antibody indeed.
Date published: 2017-02-08
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MEF-2A 抗体 (B-4) is rated 4.4 out of 5 by 22.
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