Date published: 2025-9-6
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MDR1/ABCB1 Antibody (D-11) - Western Blotting - Image 375249
MDR1 抗体 (D-11): sc-55510. MES-SA/Dx5 全细胞裂解液 (A) 和人肾上腺组织提取物 (B) 中 MDR1 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
MDR1 抗体 (D-11): sc-55510. MES-SA/Dx5 全细胞裂解液 (A) 和人肾上腺组织提取物 (B) 中 MDR1 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

MDR1/ABCB1 抗体 (D-11): sc-55510

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寫評論提問

说明书
  • MDR1/ABCB1 抗体 D-11 是小鼠单克隆 IgG2b κ，MDR1/ABCB1抗体, 在189篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Mdr-1的氨基酸1040-1280
  • MDR1/ABCB1 抗体 (D-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 MDR1; 可能与 Mdr-3产生交联反应
  • 抗MDR1/ABCB1抗体（D-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MDR1/ABCB1 (D-11): sc-55510的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 MDR1/ABCB1 抗体 (D-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MDR1/ABCB1 抗体 (D-11) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 MDR 抗体包括 MDR1 和 Mdr-3

MDR1 抗体 (D-11) 是一种小鼠单克隆 IgG2b kappa 轻链抗体，通过识别 1040-1280 氨基酸表位，设计用于靶向人 MDR1 蛋白（又称 ABCB1）。MDR1 单克隆抗体（D-11）可利用免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附试验（ELISA）等技术有效检测小鼠、大鼠和人等多个物种的 MDR1，还可能与小鼠同源物 Mdr-3 发生交叉反应。MDR1 蛋白又称 ABCB1、P-GP、PGY1、ABC20、CD243、ENPAT、GP170 和 p-170，是 ATP 结合盒（ABC）转运体家族的重要成员，主要存在于血脑屏障、肝脏、肾脏和肠道细胞的顶端膜中。MDR1 的主要功能是充当药物转运泵，主动将各种化疗药物和其他异生物体排出细胞外，这在癌症治疗中多重耐药性（MDR）的产生中起着重要作用。通过维护血脑屏障的保护屏障，MDR1 可防止潜在有害物质在大脑中积聚，从而保护神经组织。了解并准确检测 MDR1 对致力于阐明耐药机制的研究人员以及开发克服各种疾病治疗难题的策略至关重要。抗 MDR1 抗体 (D-11) 有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和一系列 Alexa Fluor® 共轭物，为各种实验应用提供了多种选择。MDR1 (D-11) 抗体有助于详细研究 MDR1 的细胞定位和功能，是推动药理学、肿瘤学和神经生物学研究不可或缺的工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MDR1/ABCB1 抗体 (D-11) 参考文献:

  1. 阿霉素耐药肿瘤细胞中与 mdr-1 过表达相关的 ras 和 myc 表达下调。  |  Banerjee, S., et al. 1992. Cell Mol Biol. 38: 561-70. PMID: 1363885
  2. Hedgehog-Gli2信号通过调节MDR1促进卵巢癌细胞的耐药性。  |  Nakamura, T. 2003. Yakugaku Zasshi. 123: 773-9. PMID: 14513768
  3. MIF 和 GSTpi 对 MDR-1 基因的调节与激素独立型前列腺癌耐药性的产生。  |  Yu, DS., et al. 2006. Arch Androl. 52: 283-91. PMID: 16728344
  4. Cyp3A4, Cyp3A5 和 MDR-1 基因对肾移植受者他克莫司药代动力学的影响。  |  Roy, JN., et al. 2006. Pharmacogenet Genomics. 16: 659-65. PMID: 16906020
  5. [MDR 1 基因是化疗成功的关键吗？］  |  Wilczyński, JR., et al. 2006. Ginekol Pol. 77: 476-84. PMID: 16964701
  6. 对MDR1过表达细胞系的研究，以获得使用体外外排活性预测脑部分布的定量方法。  |  Lim, JS., et al. 2019. J Korean Med Sci. 34: e100. PMID: 30940996
  7. 糖基转移酶MDR1为线粒体增殖组装一个分裂环，该分裂环由多聚葡聚糖纳米丝组成。  |  Wang, Q., et al. 2021. Front Oncol. 11: 794959. PMID: 35059317
  8. 通过对国家癌症研究所抗癌药物筛选细胞系中的 mdr-1/P 糖蛋白进行定量分析，生成耐药性概况。  |  Alvarez, M., et al. 1995. J Clin Invest. 95: 2205-14. PMID: 7738186
  9. 与 MDR 相关的 P170 糖蛋白可调节同金丝桃碱的细胞毒性活性。  |  Russo, D., et al. 1995. Leukemia. 9: 513-6. PMID: 7885049
  10. 免疫系统细胞中的 P-糖蛋白（MDR 1 基因产物）：在衰老和人类免疫缺陷病毒-1（HIV-1）感染中的可能生理作用和改变。  |  Gupta, S. and Gollapudi, S. 1993. J Clin Immunol. 13: 289-301. PMID: 7902361
  11. 人类多药耐药性相关蛋白 MRP 是一种质膜药物外流泵。  |  Zaman, GJ., et al. 1994. Proc Natl Acad Sci U S A. 91: 8822-6. PMID: 7916458
  12. 分析人多药耐药性肺癌细胞系中 MRP（一种新的假定跨膜药物转运体的基因）的表达。  |  Zaman, GJ., et al. 1993. Cancer Res. 53: 1747-50. PMID: 8467491

订购信息

产品名称产品编号规格价格数量收藏夹

MDR1/ABCB1 抗体 (D-11)

sc-55510
200 µg/ml
RMB2377.00

MDR1/ABCB1 (D-11): m-IgG Fc BP-HRP 套装

sc-528593
200 µg Ab; 10 µg BP
RMB2662.00

MDR1/ABCB1 (D-11): m-IgGκ BP-HRP 套装

sc-521007
200 µg Ab, 40 µg BP
RMB2662.00

MDR1/ABCB1 (D-11): m-IgG2b BP-HRP 套装

sc-550055
200 µg Ab; 10 µg BP
RMB2662.00

MDR1/ABCB1 抗体 (D-11) AC

sc-55510 AC
500 µg/ml, 25% agarose
RMB3129.00

MDR1/ABCB1 抗体 (D-11) HRP

sc-55510 HRP
200 µg/ml
RMB2377.00

MDR1/ABCB1 抗体 (D-11) FITC

sc-55510 FITC
200 µg/ml
RMB2482.00

MDR1/ABCB1 抗体 (D-11) PE

sc-55510 PE
200 µg/ml
RMB2580.00

MDR1/ABCB1 抗体 (D-11) Alexa Fluor® 488

sc-55510 AF488
200 µg/ml
RMB2685.00

MDR1/ABCB1 抗体 (D-11) Alexa Fluor® 546

sc-55510 AF546
200 µg/ml
RMB2685.00

MDR1/ABCB1 抗体 (D-11) Alexa Fluor® 594

sc-55510 AF594
200 µg/ml
RMB2685.00

MDR1/ABCB1 抗体 (D-11) Alexa Fluor® 647

sc-55510 AF647
200 µg/ml
RMB2685.00

MDR1/ABCB1 抗体 (D-11) Alexa Fluor® 680

sc-55510 AF680
200 µg/ml
RMB2685.00

MDR1/ABCB1 抗体 (D-11) Alexa Fluor® 790

sc-55510 AF790
200 µg/ml
RMB2685.00

One of our customers, who is working with cat # sc-55510, lot # C2415 is facing some issues with the antibody. The band of MDR1 is suppose to come at 170KDa but it is coming around 55KDa. They used1:1000 dilution in 2% BSA in Tbst. Please suggest.

Asked by: Rinky
Thank you for contacting Santa Cruz Biotechnology. Please contact technical service by email at [email protected] or call (800)-457-3801.
Answered by: BlakeJ
Date published: 2024-07-09

Can I get a free sample of this antibody

Asked by: Sulay
Yes! For a limited time, we are offering a free 10 µg sample of this antibody, Mdr-1 (D-11): sc-55510. Please contact our Technical Service Department to request a free sample. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-07-25

Is this antibody resistant to formalin fixation and periodate oxidation? Any published reference detailing its validation (eg epitope recognized, selectivity against other transporters)? Thks in advance.

Asked by: jmnic
Thank you for your question. This antibody, Mdr-1 (D-11): sc-55510, is not resistant to formalin fixation as it has been successfully tested on formalin-fixed paraffin-embedded tissue sections. This data is available on our website. The antibody has been cited in over 48 publications, however we are not aware of any of these publications describing the exact epitope recognized by this clone, or experimental validation being published for cross reactivity with other transporters. The complete list of product citations is available on our website for your reference.
Answered by: Technical Support
Date published: 2017-07-20

我用你们的抗体做了MDR1的WB，在140左右无条带，在55~70之间有很明显的两条条带，是杂带么？为什么有两条？

Asked by: kuaixingwang
感谢您的提问。WB实验中，实际测得的条带大小可能与预测的不符，而导致这种现象的原因很多。请您将您的实验结果发送至我们的技术支持部门，我们将为您具体分析实验中出现这种情况的原因。亚洲地区技术支持：电话800-820-8626，邮箱[email protected]。 Thank you for your inquiry. The actual band size can be different from that predicted in WB, and there are many causes for it. We would be happy to help you figure this out. Please contact our Asian Technical Services team at 800-820-8626 or [email protected]. We will do our best to help you achieve the best results possible!
Answered by: Technical Support 14
Date published: 2021-03-09

Hi We are using the Mdr-1 antibody and we repeatedly see three band (in two different cell lines): 1- 130-200 kDa smear which is supposed to be the Mdr-1 band. 2 and 3- two separated narrow bands between 35-50 kDa. How could you explain that?

Asked by: Eliz
Thank you for your question. We do not have any information about the type of lower molecular weight bands you have described when using this Mdr-1 antibody. We would recommend first performing a secondary antibody control, where you incubate your membrane without primary antibody to determine if any of those bands may be a result of non-specific binding of the secondary antibody alone.
Answered by: Technical Support
Date published: 2025-02-18

What is the recommended secondary reagent to be used with Mdr-1 Antibody (D-11) in Western blot?

Asked by: cjMara
Thank you for your question. Mdr-1 Antibody (D-11) is a mouse monoclonal IgG2b with a kappa light chain. Therefore, we would recommend using our mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Horseradish Peroxidase (HRP): sc-516102. A link to the product page is provided below for your reference. https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp You can also use a conventional anti-mouse IgG-HRP conjugated secondary antibody for detection.
Answered by: Technical Support
Date published: 2016-12-22

We want to order your anti MDR1 mouse antibody. The band which you show at the site looks like a smear between 130-200kDa. How could this be explained? Thank you in advance. Dina

Asked by: Dina
Thank you for your question. Mdr-1 Antibody (D-11): sc-55510 is a mouse monoclonal antibody which recognizes Mdr-1 of human origin by Western blot. The Mdr-1 protein has a predicted molecular weight of 170 kDa, however it has been described as hyperglycosylated and can appear as a diffuse band by Western blot (reference PMID: 12138126). This antibody is very popular and has been cited in over 40 publications, primarily for use in Western blot. A list of these product citations is available on our website for this antibody.
Answered by: Technical Support
Date published: 2017-01-27
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Rated 5 out of 5 by from Great bands observedThis antibody allowed us to perform the essays needed for our research. Best results were achieved incubating the samples with sample buffer at 40°C for 30 min instead of boiling.
Date published: 2022-07-28
Rated 4 out of 5 by from SIRTs inhibitors and sorafenibCambinol and EX-527 affect the expression of some ABC transporters during sorafenib treatment
Date published: 2021-02-26
Rated 5 out of 5 by from reliable productWe made western blot in liver isolated from Swiss mice. This monoclonal antibody is very sensitive, we used it in a 1/800 dilution and obtained good bands. I recommend it.
Date published: 2019-12-05
Rated 5 out of 5 by from Great signal!The antibody worked perfectly, I could even detect a good signal with 1:800 dilution.
Date published: 2019-11-27
Rated 1 out of 5 by from no WB signal in MCF7 cellsNo signal in total cell lysates from MCF7 cells. Tested by twi different lysis protocols (SDS 1% + sample boiling) and RIPA buffer with no boiling. No bands at the supoosed MDR1 molecular weight.
Date published: 2019-10-31
Rated 5 out of 5 by from Excellent antibodyThis antibody works great for Immunohistochemistry.
Date published: 2019-05-20
Rated 1 out of 5 by from Did not work for WB analysisTried at a 1:500 dilution in human and canine bladder cancer cells but detected no signal.
Date published: 2018-06-19
Rated 4 out of 5 by from Great!This MDR-1 antibody is great, especially comparing to the other one (G-1).
Date published: 2018-01-30
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MDR1/ABCB1 抗体 (D-11) is rated 4.2 out of 5 by 33.
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