Date published: 2025-9-6

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MCP-1-4/eotaxin 抗体 (B-2): sc-377082

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说明书
  • MCP-1-4/eotaxin 抗体 B-2 是小鼠单克隆 IgG1 κ,MCP-1-4/eotaxin抗体, 在3篇文献中引用,规格为200 µg/ml
  • 针对代表human起源的全长MCP-1的氨基酸1-99
  • MCP-1-4/eotaxin 抗体 (B-2) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 MCP-1, MCP-2, MCP-3, MCP-4 and eotaxin
  • 抗MCP-1-4/eotaxin抗体(B-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MCP-1-4/eotaxin (B-2): sc-377082免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRPm-IgGκ BP-HRP 是 MCP-1-4/eotaxin 抗体 (B-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MCP-1-4/eotaxin 抗体 (B-2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

MCP-1-4/eotaxin抗体(B-2)是一种IgG1 κ小鼠单克隆MCP-1-4/eotaxin抗体(也称为CCL2(MCP-1)抗体、CCL8(MCP-2)抗体、CCL7(MCP-3)抗体、CCL13(MCP-4)抗体、CCL11(eotaxin)抗体或C-C模体趋化因子配体抗体),它能够通过WB、IP、IF、IHC(P)和ELISA检测人源MCP-1-4/eotaxin蛋白。MCP-1-4/eotaxin抗体(B-2)既可作为非偶联形式的抗MCP-1-4/eotaxin抗体,也可作为多种偶联形式的抗MCP-1-4/eotaxin抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。嗜酸性粒细胞趋化因子和单核细胞趋化蛋白MCP-1–5构成了C-C(或β)趋化因子的一个亚家族,其特点是一组保守的相邻半胱氨酸。MCPs由多种细胞产生,包括T淋巴细胞,这些细胞随后被IL-1、TNFα和IFN-γ等细胞因子激活。体外研究表明,MCP异构体对不同的淋巴细胞亚群表现出趋化作用。MCP-1是一种有效的嗜碱性粒细胞激活剂,但不影响嗜酸性粒细胞。MCP-1水平在感染和炎症期间会增加,而这两者都以白细胞浸润为特征。已经鉴定出两种MCP-1受体,它们在羧基末端有所不同。MCP-2能刺激嗜酸性粒细胞和嗜碱性粒细胞,而eotaxin则作为嗜酸性粒细胞的强效趋化剂。MCP-3已被证明具有最广泛的影响范围,能激活单核细胞、树突状细胞、淋巴细胞、NK细胞、嗜酸性粒细胞、嗜碱性粒细胞和中性粒细胞。MCP-4通过CCR-2和CCR-3发出信号,是过敏性炎症和非过敏性炎症中诱导的单核细胞、嗜酸性粒细胞和嗜碱性粒细胞的有效趋化剂。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MCP-1-4/eotaxin 抗体 (B-2) 参考文献:

  1. 静脉注射抗坏血酸疗法对癌症患者细胞因子的调节。  |  Mikirova, N., et al. 2016. Med Sci Monit. 22: 14-25. PMID: 26724916
  2. 炎症细胞因子和趋化因子与患者预后和急性脑损伤后的高肾上腺素状态有关。  |  Di Battista, AP., et al. 2016. J Neuroinflammation. 13: 40. PMID: 26883121
  3. 视神经脊髓炎谱系疾病中嗜酸性粒细胞趋化因子和细胞因子水平升高。  |  Du, L., et al. 2021. Mult Scler Relat Disord. 52: 102940. PMID: 33930716
  4. 单核细胞趋化蛋白 MCP-2 与 MCP-3 类似,能激活人类嗜碱性粒细胞和嗜酸性粒细胞。  |  Weber, M., et al. 1995. J Immunol. 154: 4166-72. PMID: 7535823
  5. 单核细胞趋化蛋白-1(MCP-1), -2 和-3 对人类 T 淋巴细胞具有趋化作用。  |  Taub, DD., et al. 1995. J Clin Invest. 95: 1370-6. PMID: 7883984
  6. 两种单核细胞趋化蛋白 1 受体的分子克隆和功能表达揭示了羧基末端尾部的替代剪接。  |  Charo, IF., et al. 1994. Proc Natl Acad Sci U S A. 91: 2752-6. PMID: 8146186
  7. 单核细胞趋化蛋白-3 是 CC 趋化因子受体 1 和 2B 的功能配体。  |  Combadiere, C., et al. 1995. J Biol Chem. 270: 29671-5. PMID: 8530354
  8. 人类单核细胞趋化蛋白-2 和-3:与 MCP-1 的结构和功能比较。  |  Proost, P., et al. 1996. J Leukoc Biol. 59: 67-74. PMID: 8558070
  9. 季节性过敏性鼻炎中的趋化因子  |  Kuna, P., et al. 1996. J Allergy Clin Immunol. 97: 104-12. PMID: 8568122
  10. 小鼠 T 细胞杂交种中趋化因子单核细胞趋化蛋白-1 受体的早期信号转导。  |  Dubois, PM., et al. 1996. J Immunol. 156: 1356-61. PMID: 8568234
  11. 单核细胞趋化蛋白-1 的定点突变确定了多肽中对生物活性至关重要的两个区域。  |  Beall, CJ., et al. 1996. Biochem J. 313 (Pt 2): 633-40. PMID: 8573103
  12. 促炎性细胞因子调节体外人类环状细胞中的趋化因子CCL2(MCP-1):CCL2的表达和产生  |  Helen E. Gruber, et al. 2015. ELSEVIER. 98: 102-105.

订购信息

产品名称产品编号规格价格数量收藏夹

MCP-1-4/eotaxin 抗体 (B-2)

sc-377082
200 µg/ml
RMB2377.00

MCP-1-4/eotaxin (B-2): m-IgGκ BP-HRP 套装

sc-523178
200 µg Ab, 40 µg BP
RMB2662.00

MCP-1-4/eotaxin (B-2): m-IgG1 BP-HRP 套装

sc-533560
200 µg Ab; 20 µg BP
RMB2662.00

MCP-1-4/eotaxin 抗体 (B-2) AC

sc-377082 AC
500 µg/ml, 25% agarose
RMB3129.00

MCP-1-4/eotaxin 抗体 (B-2) HRP

sc-377082 HRP
200 µg/ml
RMB2377.00

MCP-1-4/eotaxin 抗体 (B-2) FITC

sc-377082 FITC
200 µg/ml
RMB2482.00

MCP-1-4/eotaxin 抗体 (B-2) PE

sc-377082 PE
200 µg/ml
RMB2580.00

MCP-1-4/eotaxin 抗体 (B-2) Alexa Fluor® 488

sc-377082 AF488
200 µg/ml
RMB2685.00

MCP-1-4/eotaxin 抗体 (B-2) Alexa Fluor® 546

sc-377082 AF546
200 µg/ml
RMB2685.00

MCP-1-4/eotaxin 抗体 (B-2) Alexa Fluor® 594

sc-377082 AF594
200 µg/ml
RMB2685.00

MCP-1-4/eotaxin 抗体 (B-2) Alexa Fluor® 647

sc-377082 AF647
200 µg/ml
RMB2685.00

MCP-1-4/eotaxin 抗体 (B-2) Alexa Fluor® 680

sc-377082 AF680
200 µg/ml
RMB2685.00

MCP-1-4/eotaxin 抗体 (B-2) Alexa Fluor® 790

sc-377082 AF790
200 µg/ml
RMB2685.00

Can MCP-1-4/eotaxin (B-2): sc-377082 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, MCP-1-4/eotaxin (B-2): sc-377082 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from okay antibodyI tried this antibody in the ratio 1:1000 to detect CCL2 in NHBE whole cell lysate and luckily we got a band around 12kDa for the first time. But after that we tried in Beas2b cell lysate and again in NHBE lysate too but we couldn't see the band. Maybe it will work only in freshly prepared antibody so its not a user-friendly.
Date published: 2017-03-14
Rated 5 out of 5 by from only for WBwe do human samples stimulated by IL1B, and we see spicific band at 11kD, and also there is a none spicific band at 40kD. But we do not consider it could do IHC-P.
Date published: 2016-12-14
Rated 2 out of 5 by from Robust band seen in WB using human lung tissueRobust band seen in WB using human lung tissue extract. -SCBT QC
Date published: 2015-04-20
Rated 4 out of 5 by from Worked with transfected lysate by WBWorked with transfected lysate by WB -SCBT QC
Date published: 2015-02-03
Rated 5 out of 5 by from Published WB and IHC data with mouse samplesPublished WB and IHC data with mouse samples - SCBT Publication Review
Date published: 2015-01-19
Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmicExceptional immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human upper stomach tissue. -SCBT QC
Date published: 2013-01-18
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MCP-1-4/eotaxin 抗体 (B-2) is rated 4.0 out of 5 by 6.
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