Date published: 2025-9-7
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MCM3 Antibody (E-8) - Western Blotting - Image 374386
MCM3 抗体 (E-8): sc-390480. K-562 (A), COLO 205 (B), A-431 (C), HeLa (D), F9 (E) 和 3T3-L1 (F) 全细胞裂解液中 MCM3 表达的 Western 印迹分析.
MCM3 抗体 (E-8): sc-390480. K-562 (A), COLO 205 (B), A-431 (C), HeLa (D), F9 (E) 和 3T3-L1 (F) 全细胞裂解液中 MCM3 表达的 Western 印迹分析.

MCM3 抗体 (E-8): sc-390480

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说明书
  • MCM3 抗体 E-8 是小鼠单克隆 IgG1 κ，MCM3抗体, 在15篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1-215位于human物种的MCM3的N-terminus
  • MCM3 抗体 (E-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 MCM3; 也和以下物种反应，包括： canine
  • 抗MCM3抗体（E-8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MCM3 (E-8): sc-390480的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 MCM3 抗体 (E-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MCM3 抗体 (E-8) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
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说明书与实验方案
研究信息

関連項目

此外 MCM 抗体 包括 MCM2, MCM3, MCM4, MCM5, MCM6和MCM7

MCM3抗体（E-8）是一种IgG1 κ小鼠单克隆MCM3抗体，可通过WB、IP、IF、IHC(P)和ELISA等方法检测小鼠、大鼠和人类来源的MCM3蛋白。MCM3抗体（E-8）既有非偶联形式的抗MCM3抗体，也有多种偶联形式的抗MCM3抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。微小染色体维持（MCM）蛋白家族，包括MCM2、MCM3、MCM4（Cdc21）、MCM5（Cdc46）、MCM6（Mis5）和MCM7（Cdc47），是DNA复制的调节因子，其作用是确保在细胞周期中仅复制一次。MCM蛋白的表达在细胞生长期间增加，在G1到S期达到峰值。每个MCM蛋白都包含一个ATP结合基序，该基序预计会介导依赖于ATP的双链DNA的开放。MCM蛋白受E2F转录因子的调节，E2F转录因子诱导MCM表达，并受蛋白激酶的调节，蛋白激酶与MCM蛋白相互作用以维持细胞的复制后状态。MCM2 / MCM4复合物在体外作为Cdc2 /细胞周期蛋白B的底物起作用。MCM3的裂解（可通过半胱氨酸蛋白酶抑制剂预防）导致至少由MCM2-6组成的MCM复合物在凋亡期间失活。已显示由MCM4、MCM6和MCM7组成的复合物参与DNA解旋酶活性，而MCM5参与IFN-γ诱导的Stat1α转录激活。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MCM3 抗体 (E-8) 参考文献:

  1. MCM3 作为良性和恶性唾液腺肿瘤的新型诊断标记物。  |  Ashkavandi, ZJ., et al. 2013. Asian Pac J Cancer Prev. 14: 3479-82. PMID: 23886132
  2. 肝细胞癌中 MCM3 及其互作蛋白的诊断和预后价值。  |  Li, HT., et al. 2020. Oncol Lett. 20: 308. PMID: 33093917
  3. MCM3 上调会导致乳腺癌的内分泌抗药性，是他莫昔芬疗效减弱的预测标志。  |  Løkkegaard, S., et al. 2021. NPJ Breast Cancer. 7: 2. PMID: 33398005
  4. 肝细胞癌中 MCM3 和 Glypican-3 分离及联合表达的诊断和预后价值：一种新的免疫分型预后模型。  |  Mohamed, AS., et al. Appl Immunohistochem Mol Morphol. 30: 694-702. PMID: 36350065
  5. 包括参与真核生物 DNA 复制起始过程的 MCM 蛋白在内的各种假定的依赖核酸的 ATP 酶中存在一组共同的保守基序。  |  Koonin, EV. 1993. Nucleic Acids Res. 21: 2541-7. PMID: 8332451
  6. DNA 螺旋酶的活性与 MCM4, -6 和 -7 蛋白复合物有关。  |  Ishimi, Y. 1997. J Biol Chem. 272: 24508-13. PMID: 9305914
  7. 在 G2 阶段抑制蛋白激酶可使哺乳动物的 Mcm 蛋白与染色质重新结合，恢复复制能力。  |  Coverley, D., et al. 1998. Exp Cell Res. 238: 63-9. PMID: 9457057
  8. 核复制因子 MCM3 在细胞凋亡过程中的选择性蛋白水解。  |  Schwab, BL., et al. 1998. Exp Cell Res. 238: 415-21. PMID: 9473350
  9. 细胞周期和染色质结合状态依赖的人类 MCM 异六聚体复合物磷酸化。cdc2 激酶的作用。  |  Fujita, M., et al. 1998. J Biol Chem. 273: 17095-101. PMID: 9642275
  10. E2F3 的活性在细胞周期中受到调控，是诱导 S 期所必需的。  |  Leone, G., et al. 1998. Genes Dev. 12: 2120-30. PMID: 9679057
  11. 在 IFN-gamma 诱导的转录激活过程中，Stat1alpha 对 MCM5 的招募依赖于 Ser727。  |  Zhang, JJ., et al. 1998. EMBO J. 17: 6963-71. PMID: 9843502
  12. 人类 T 细胞白血病病毒 1 型税蛋白可减轻小鼠 T 细胞系对白细胞介素-2 的依赖性。  |  Iwanaga, Y., et al. 1999. J Virol. 73: 1271-7. PMID: 9882331

订购信息

产品名称产品编号规格价格数量收藏夹

MCM3 抗体 (E-8)

sc-390480
200 µg/ml
RMB2377.00

MCM3 (E-8): m-IgG Fc BP-HRP 套装

sc-530164
200 µg Ab; 10 µg BP
RMB2662.00

MCM3 (E-8): m-IgGκ BP-HRP 套装

sc-523550
200 µg Ab, 40 µg BP
RMB2662.00

MCM3 (E-8): m-IgG1 BP-HRP 套装

sc-543903
200 µg Ab; 20 µg BP
RMB2662.00

MCM3 抗体 (E-8) AC

sc-390480 AC
500 µg/ml, 25% agarose
RMB3129.00

MCM3 抗体 (E-8) HRP

sc-390480 HRP
200 µg/ml
RMB2377.00

MCM3 抗体 (E-8) FITC

sc-390480 FITC
200 µg/ml
RMB2482.00

MCM3 抗体 (E-8) PE

sc-390480 PE
200 µg/ml
RMB2580.00

MCM3 抗体 (E-8) Alexa Fluor® 488

sc-390480 AF488
200 µg/ml
RMB2685.00

MCM3 抗体 (E-8) Alexa Fluor® 546

sc-390480 AF546
200 µg/ml
RMB2685.00

MCM3 抗体 (E-8) Alexa Fluor® 594

sc-390480 AF594
200 µg/ml
RMB2685.00

MCM3 抗体 (E-8) Alexa Fluor® 647

sc-390480 AF647
200 µg/ml
RMB2685.00

MCM3 抗体 (E-8) Alexa Fluor® 680

sc-390480 AF680
200 µg/ml
RMB2685.00

MCM3 抗体 (E-8) Alexa Fluor® 790

sc-390480 AF790
200 µg/ml
RMB2685.00

I am using MCM3 (E-8): sc-390480 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Very specific band at about 115kDaI used Goat anti MCM3 previously, sc9850, but it's finished. By changing this new one, I can obtain the exact specific band at about 115kDa against mouse cell lysates but haven't tried in tissue proteins. I dilute at 1:1000 in 5%milk/TBST.
Date published: 2021-06-29
Rated 5 out of 5 by from Great antibodyWe use this antibody 1:500 for western blotting detection of MCM3. Higly specific.
Date published: 2019-08-23
Rated 3 out of 5 by from It works in some samplesIt worked well in our samples. But somehow it didn't work in some of our samples compared to other company.
Date published: 2018-06-29
Rated 4 out of 5 by from This antibody performed wellThis antibody performed well. But we found two bands in our human sample, one is around 75 kDa and another one is around 115 kDa where the datasheet points out.
Date published: 2018-04-19
Rated 5 out of 5 by from 抗体可以的very 基本没有杂带，牛奶封闭，一抗过夜，二抗牛奶室温两小时，30ug细胞蛋白，跑出条带如下图所示
Date published: 2018-03-11
Rated 4 out of 5 by from Clean Western data & reasonable IF dataI used this antibody in my western blotting (1 to 200 dilution) and IF (1 to 100 dilution). In western, I used whole cell extracts from hTERT-RPE1 cells and I could get a nice clean single band. As for IF, this MCM3 antibody showed neat data as good as MCM7 antibody (sc-56324), however, signal strength looked weaker than anti-MCM7. If I'd see any MCMs, I choose MCM7 antibody though if I'd specifically see MCM3, I'll consider using this antibody.
Date published: 2017-11-10
Rated 5 out of 5 by from a good antibody for WBThis antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from One of the best antibodies for WBOne of the best antibodies for WB that I have ever used. Single band at about 120 kd. Now we are trying IP experiments.
Date published: 2017-08-22
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MCM3 抗体 (E-8) is rated 4.6 out of 5 by 15.
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