Date published: 2025-9-8
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Mcl-1 Antibody (22) - Western Blotting - Image 393403
Mcl-1 抗体 (22) Alexa Fluor® 647: sc-12756 AF647. 对 U-698-M 全细胞裂解液 (A) 和 U266 (B) 全细胞裂解液及人扁桃体组织提取物 (C) 中 Mcl-1 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker MW Tag-Alexa Fluor® 488 检测 Cruz Marker™ 分子量标准: sc-516790.
Mcl-1 抗体 (22) Alexa Fluor® 647: sc-12756 AF647. 对 U-698-M 全细胞裂解液 (A) 和 U266 (B) 全细胞裂解液及人扁桃体组织提取物 (C) 中 Mcl-1 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker MW Tag-Alexa Fluor® 488 检测 Cruz Marker™ 分子量标准: sc-516790.

Mcl-1 抗体 (22): sc-12756

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说明书
  • Mcl-1 抗体 22 是小鼠单克隆 IgG1 κ，Mcl-1抗体, 在167篇文献中引用，规格为200 µg/ml
  • 针对全长Mcl-1，human起源
  • Mcl-1 抗体 (22) 推荐用于WB, IP 和 ELISA，检测human 来源的 Mcl-1 long and short forms
  • 抗Mcl-1抗体（22）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素(sc-12756 B, 200 µg/ml)偶联抗体，可用于WB、IHC(P)和ELISA实验
  • 关于如何获取Mcl-1 (22): sc-12756的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Mcl-1 Antibody (22) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Mcl-1 Antibody (22) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Mcl-1抗体（22）是一种鼠单克隆IgG1 kappa轻链抗体，专门针对全长人Mcl-1蛋白，可通过蛋白质印迹（WB）、免疫沉淀（IP）和酶联免疫吸附测定（ELISA）有效检测人样本中的长和短Mcl-1形式。Mcl-1，也称为骨髓细胞白血病-1、Mcl1和Gm52627，是Bcl-2蛋白家族的重要成员，该家族是细胞凋亡的关键调节因子。Mcl-1是一种抗凋亡蛋白，通过与促凋亡因子（如Bak和Bax）结合并抑制其活性来促进细胞存活，从而防止线粒体外膜通透性和细胞色素c的释放。这一功能在造血系统中尤为重要，因为Mcl-1对于维持早期祖细胞的稳定至关重要。Mcl-1的失调和过度表达与多种癌症的发生和发展有关，包括白血病、淋巴瘤和实体瘤，因为Mcl-1可使恶性细胞逃避凋亡并继续不受控制地增殖。Mcl-1的稳定性和活性受多种信号通路调节，包括PI3K/Akt通路和糖原合成酶激酶3（GSK3），这使得Mcl-1成为癌症治疗中重要的治疗干预靶点。因此，了解和检测Mcl-1的表达水平对于癌症研究和药物开发至关重要。Mcl-1 (22)抗体有未结合形式和与琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®结合物的结合形式，为研究人员提供了在各种实验应用中研究Mcl-1的多功能工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Mcl-1 抗体 (22) 参考文献:

  1. Mcl-1 缺乏会导致胚胎着床周围死亡。  |  Rinkenberger, JL., et al. 2000. Genes Dev. 14: 23-7. PMID: 10640272
  2. MCL-1S 是抗凋亡 BCL-2 家族成员 MCL-1 的剪接变体，编码一种仅具有 BH3 结构域的促凋亡蛋白。  |  Bae, J., et al. 2000. J Biol Chem. 275: 25255-61. PMID: 10837489
  3. B 淋巴细胞和 T 淋巴细胞的发育和维持需要抗凋亡的 MCL-1。  |  Opferman, JT., et al. 2003. Nature. 426: 671-6. PMID: 14668867
  4. 线粒体 p53 激活 Bak，并导致 Bak-Mcl1 复合物的破坏。  |  Leu, JI., et al. 2004. Nat Cell Biol. 6: 443-50. PMID: 15077116
  5. 抗凋亡 MCL-1 在造血干细胞的存活中发挥着不可或缺的作用。  |  Opferman, JT., et al. 2005. Science. 307: 1101-4. PMID: 15718471
  6. 糖原合成酶激酶-3 通过破坏 MCL-1 的稳定性来调节线粒体外膜通透性和细胞凋亡。  |  Maurer, U., et al. 2006. Mol Cell. 21: 749-60. PMID: 16543145
  7. MCL-1 抑制剂，一类新型抗癌药物的快速发展。  |  Bolomsky, A., et al. 2020. J Hematol Oncol. 13: 173. PMID: 33308268
  8. Mcl-1 抑制剂：治疗多发性骨髓瘤中的恶性肿瘤。  |  Al-Odat, O., et al. 2021. Front Pharmacol. 12: 699629. PMID: 34349655
  9. 靶向 MCL-1 蛋白治疗癌症：机遇与挑战。  |  Tantawy, SI., et al. 2023. Front Oncol. 13: 1226289. PMID: 37601693
  10. 抗凋亡 MCL-1 通过与 ACSL1 相互作用促进长链脂肪酸氧化  |  Wright, T., et al. 2024. Mol Cell. 84: 1338-1353.e8. PMID: 38503284
  11. MCL1 是一种在程序性髓系细胞分化过程中表达的基因，其序列与 BCL2 相似。  |  Kozopas, KM., et al. 1993. Proc Natl Acad Sci U S A. 90: 3516-20. PMID: 7682708
  12. 髓系细胞白血病-1 基因的人类和小鼠染色体图谱：MCL1 映射到人类染色体 1q21，该区域在肿瘤前病变和肿瘤性疾病中经常发生变化。  |  Craig, RW., et al. 1994. Genomics. 23: 457-63. PMID: 7835896

订购信息

产品名称产品编号规格价格数量收藏夹

Mcl-1 抗体 (22)

sc-12756
200 µg/ml
RMB2377.00

Mcl-1 (22): m-IgG Fc BP-HRP 套装

sc-528257
200 µg Ab; 10 µg BP
RMB2662.00

Mcl-1 (22): m-IgGκ BP-HRP 套装

sc-520588
200 µg Ab, 40 µg BP
RMB2662.00

Mcl-1 (22): m-IgG1 BP-HRP 套装

sc-542844
200 µg Ab; 20 µg BP
RMB2662.00

Mcl-1 抗体 (22) AC

sc-12756 AC
500 µg/ml, 25% agarose
RMB3129.00

Mcl-1 抗体 (22) HRP

sc-12756 HRP
200 µg/ml
RMB2377.00

Mcl-1 抗体 (22) FITC

sc-12756 FITC
200 µg/ml
RMB2482.00

Mcl-1 抗体 (22) PE

sc-12756 PE
200 µg/ml
RMB2580.00

Mcl-1 抗体 (22) Alexa Fluor® 488

sc-12756 AF488
200 µg/ml
RMB2685.00

Mcl-1 抗体 (22) Alexa Fluor® 546

sc-12756 AF546
200 µg/ml
RMB2685.00

Mcl-1 抗体 (22) Alexa Fluor® 594

sc-12756 AF594
200 µg/ml
RMB2685.00

Mcl-1 抗体 (22) Alexa Fluor® 647

sc-12756 AF647
200 µg/ml
RMB2685.00

Mcl-1 抗体 (22) Alexa Fluor® 680

sc-12756 AF680
200 µg/ml
RMB2685.00

Mcl-1 抗体 (22) Alexa Fluor® 790

sc-12756 AF790
200 µg/ml
RMB2685.00

Mcl-1 抗体 (22) B

sc-12756 B
200 µg/ml
RMB2407.00

Dear Sir or Madam, I have tried this antibody on a panel of cell lines, one of them Jurkat which should give a strong signal. So far I only get very faint bands and plenty of unspecific ones. Do you have any advice please? ,Thank you

Asked by: Angelika Bo
Thank you for your question. Please contact your local distributor or Technical Service directly for assistance regarding your WB results, we are happy to troubleshoot and discuss your results with you.
Answered by: Tech Service
Date published: 2019-09-02

¿Cuál es la región de la proteína que reconoce este anticuerpo?

Asked by: Xhiucoatl
Thank you for your question. This antibody, sc-12756, raised against full length Mcl-1 of human origin. The exact epitope has not been mapped, so no further information is available.
Answered by: Tech Service
Date published: 2019-05-31

Does this antibody interact with mouse MCL1 or it solely probes for human MCL1?

Asked by: bchen52
Thank you for your question. This antibody has not been tested for mouse use, and can therefore not be warrantied for this use. If you have any further questions, please contact Technical Service.
Answered by: Tech Service
Date published: 2019-02-11

Can it be used in paraffin embedded tissues for immunohistochemical analysis? As I haven't found something from the citations.

Asked by: Diman
Thank you for your question. We have not yet tested our mouse monoclonal Mcl-1 Antibody (22): sc-12756 for immunohistochemistry on formalin-fixed paraffin-embedded tissue sections. As a result, the application of IHC(P) is not currently recommended. This antibody is extremely well cited, but it is most frequently used for Western blot (WB) and immunoprecipitation (IP). We would recommend an alternative clone for IHC(P), Mcl-1 Antibody (G-7): sc-74437 (see link below). This antibody has been tested in-house and has excellent published data for IHC(P) shown on our website. https://www.scbt.com/scbt/product/mcl-1-antibody-g-7
Answered by: Technical Support
Date published: 2017-02-21

What is the best secondary to use with Mcl-1 Antibody (22): sc-12756 in Western Blot?

Asked by: jenni
Thank you for your question. We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-04
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Rated 4 out of 5 by from Great for WBThis primary antibody is useful in western blotting.
Date published: 2024-03-20
Rated 4 out of 5 by from Good for detecting endogenous proteinUsed it for immunoblot analysis in multiple cell lines with and without proteasome inhibition. Reuse of the dilution was not reliable. A fresh dilution always gave consistent results.
Date published: 2020-12-31
Rated 3 out of 5 by from WBWe used this antibody in WB (1:100). Bands were shown.
Date published: 2019-08-24
Rated 1 out of 5 by from It does not workWe ordered this antibody as alternative to rabbit polyclonal we had from the compony. We tried western blot in parallel with the antibody we had before. This monoclonal gave us no specific signal for MCL-1. We also had lysates from cells over expressing MCL-1. The antibody did not work for these lysates too.
Date published: 2018-10-25
Rated 4 out of 5 by from Have nonspecific bands, but major bands are goodWB in 76NE6 cell lysates, 1:1000 dilution, major bands detected with some nonspecific signaling.
Date published: 2018-05-21
Rated 5 out of 5 by from 效果挺好的第一次用这个牌子的抗体，实用了好几只，效果都挺好的，条带清晰，用量节省，适合初学者上手，赞！希望以后有更多这样的好产品！
Date published: 2018-05-21
Rated 5 out of 5 by from Great brandThe antibody works so well, the brand so clean and strong. We use it as 1: 500.
Date published: 2018-04-25
Rated 1 out of 5 by from Mcl-1 Antibody (#12756)we tried it many times did not work (frustrating) used at 1/200 dilution
Date published: 2018-04-10
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Mcl-1 抗体 (22) is rated 4.3 out of 5 by 28.
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