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MBP 抗体 (A-3): sc-376995

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说明书
  • MBP抗体(A-3)是小鼠单克隆IgG1 κ, 在7篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的MBP的内部区域内的氨基酸54-90之间
  • 抗-MBP 抗体 (A-3) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 MBP isoforms 3 and 4 (also designated MBP1 and MBP2)
  • 抗MBP抗体(A-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MBP (A-3): sc-376995免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是MBP Antibody (A-3) for WB applications. 的首选辅助检测试剂,这些试剂现与MBP Antibody (A-3) 打包提供(请参阅下面的订购信息)。

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関連項目

MBP抗体(A-3)是一种IgG1 κ型小鼠单克隆MBP抗体(也称为髓鞘碱性蛋白抗体),可通过WB、IP、IF和ELISA方法检测小鼠、大鼠和人类的MBP同种型3和4(也称为MBP1和MBP2)。MBP抗体(A-3)以非偶联抗MBP抗体形式提供,也提供多种偶联形式的抗MBP抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。髓鞘碱性蛋白(MBP)是中枢神经系统髓鞘的主要外在膜蛋白。MBP在苏氨酸125位的磷酸化是一个复杂的调控过程,它调节MBP对髓鞘稳定性的贡献。在髓鞘形成和脱髓鞘疾病多发性硬化症中,丝裂原活化蛋白激酶调节MBP的磷酸化。MBP的磷酸化受到海马被髓鞘包裹的输出纤维即穹窿的高频刺激调节,但不受低频刺激调节。有人提出,在神经元活动增加期间,钙激活轴突一氧化氮合酶,后者产生细胞间信使一氧化氮和超氧阴离子,并通过MAPK调节MBP的磷酸化状态。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

MBP 抗体 (A-3)

sc-376995
200 µg/ml
RMB2377.00

MBP (A-3): m-IgG Fc BP-HRP 套装

sc-527572
200 µg Ab; 10 µg BP
RMB2662.00

MBP (A-3): m-IgG1 BP-HRP 套装

sc-532945
200 µg Ab; 20 µg BP
RMB2662.00

MBP 抗体 (A-3) AC

sc-376995 AC
500 µg/ml, 25% agarose
RMB3129.00

MBP 抗体 (A-3) HRP

sc-376995 HRP
200 µg/ml
RMB2377.00

MBP 抗体 (A-3) FITC

sc-376995 FITC
200 µg/ml
RMB2482.00

MBP 抗体 (A-3) PE

sc-376995 PE
200 µg/ml
RMB2580.00

MBP 抗体 (A-3) Alexa Fluor® 488

sc-376995 AF488
200 µg/ml
RMB2685.00

MBP 抗体 (A-3) Alexa Fluor® 546

sc-376995 AF546
200 µg/ml
RMB2685.00

MBP 抗体 (A-3) Alexa Fluor® 594

sc-376995 AF594
200 µg/ml
RMB2685.00

MBP 抗体 (A-3) Alexa Fluor® 647

sc-376995 AF647
200 µg/ml
RMB2685.00

MBP 抗体 (A-3) Alexa Fluor® 680

sc-376995 AF680
200 µg/ml
RMB2685.00

MBP 抗体 (A-3) Alexa Fluor® 790

sc-376995 AF790
200 µg/ml
RMB2685.00

MBP (A-3) 中和勝肽

sc-376995 P
100 µg/0.5 ml
RMB511.00

Do we need to do antigen retrieval for this antibody? I have been using it for IHC but somehow I can't see anything.

Asked by: Poojaparishar
Thank you for your question. Please contact your local distributor for assistance in troubleshooting this antibody.
Answered by: Tech Service
Date published: 2019-08-26

What application is the blocking peptide sc-376995 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from Good work in IFThis antibody shows good detection of MBP protein in rat tissue in IF.
Date published: 2022-07-05
Rated 4 out of 5 by from Good antibodyThis antibody worked very well with fixed frozen mouse tissue sections for IF
Date published: 2018-10-03
Rated 5 out of 5 by from Clean for tissue extractsThis antibody shows good detection of MBP protein in mouse, rat and human tissue extracts in Western Blot.
Date published: 2017-01-27
Rated 5 out of 5 by from Great for IF stainingGreat for IF staining - used SCBT secondary.
Date published: 2015-09-11
Rated 5 out of 5 by from intensely stains Schwann cell myelin in mouseintensely stains Schwann cell myelin in mouse
Date published: 2015-04-28
Rated 5 out of 5 by from Nice Western blot analysis of MBP expressionNice Western blot analysis of MBP expression in mouse brain and rat brain tissue extracts. -SCBT QC
Date published: 2015-03-29
Rated 5 out of 5 by from Published WB data with mice forebrain andPublished WB data with mice forebrain and cerebellar extracts - SCBT Publication Review
Date published: 2013-04-09
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MBP 抗体 (A-3) is rated 4.6 out of 5 by 7.
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