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MBP 抗体 (201): sc-66064

4.3(4)
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说明书
  • MBP 抗体 201 是小鼠单克隆 IgG2b, 在11篇文献中引用,提供 100 µg/ml
  • 免疫human物种的MBP的氨基酸130-136
  • 推荐用于 mouse, rat 和 human 来源的MBP (human 21.5 kDa and 18.5 kDa molecular forms) WB, IP, IF 和 IHC(P)检测; 也和以下物种反应,包括: 和 rabbit, ovine, caprine and primate
  • 看 MBP (F-6): sc-271524,可查看有标记的 MBP 抗体,包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 目前,我们还没有完成MBP 抗体 (201)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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関連項目

髓鞘碱性蛋白(MBP)是中枢神经系统髓鞘的主要外在膜蛋白。MBP在苏氨酸125位的磷酸化是一个复杂的调控过程,它调节MBP对髓鞘稳定性的贡献。在髓鞘形成和脱髓鞘疾病多发性硬化症中,丝裂原活化蛋白激酶调节MBP的磷酸化。MBP的磷酸化受到海马被髓鞘包裹的输出纤维即穹窿的高频刺激调节,但不受低频刺激调节。有人提出,在神经元活动增加期间,钙激活轴突一氧化氮合酶,后者产生细胞间信使一氧化氮和超氧阴离子,并通过MAPK调节MBP的磷酸化状态。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MBP 抗体 (201) 参考文献:

  1. 髓磷脂碱性蛋白(MBP)和MBP肽是培养星形胶质细胞的促有丝分裂素。  |  South, SA., et al. 2000. Glia. 29: 81-90. PMID: 10594925
  2. 铜的吸收可诱导18.5 kDa髓磷脂碱性蛋白(MBP)的自组装。  |  Bund, T., et al. 2010. Biophys J. 99: 3020-8. PMID: 21044600
  3. 异质性核核糖核蛋白(hnRNP)F是少突胶质细胞RNA转运颗粒的一种新成分,有助于调节髓鞘碱性蛋白(MBP)的合成。  |  White, R., et al. 2012. J Biol Chem. 287: 1742-54. PMID: 22128153
  4. 18.5-kDa髓磷脂碱性蛋白(MBP)电荷成分-C1在膜上的横向自组装。  |  Kattnig, DR., et al. 2012. Biochim Biophys Acta. 1818: 2636-47. PMID: 22728818
  5. 围产期接触甲基汞后,发育中大鼠小脑髓磷脂碱性蛋白(Mbp)剪接变体的表达受到干扰。  |  Padhi, BK. and Pelletier, G. 2012. Toxicol Lett. 213: 374-80. PMID: 22835759
  6. 人类IgG如何识别髓磷脂碱性蛋白(MBP)寡肽和MBP。  |  Belov, S., et al. 2017. J Mol Recognit. 30: PMID: 28470769
  7. 定量核磁共振成像指标与脱髓鞘小鼠模型中髓磷脂碱性蛋白(MBP)染色之间的相关性。  |  Soustelle, L., et al. 2019. NMR Biomed. 32: e4116. PMID: 31225675
  8. 髓磷脂碱性蛋白(MBP)电荷变异与类髓磷脂脂质单层之间存在不同的鞘磷脂介导的相互作用。  |  Widder, K., et al. 2020. Biochim Biophys Acta Biomembr. 1862: 183077. PMID: 31805269
  9. 胆固醇和髓磷脂碱性蛋白(MBP)含量对模拟髓磷脂细胞质膜的脂质单层的影响。  |  Träger, J., et al. 2020. Cells. 9: PMID: 32106542
  10. 反复注射吗啡后服用伊博格碱可上调Sprague Dawley大鼠内囊中髓鞘化标志物2', 3'- 环核苷酸3'-磷酸二酯酶(CNP)和髓鞘碱性蛋白(MBP)mRNA和蛋白的表达。  |  Govender, D., et al. 2024. Front Neurosci. 18: 1378841. PMID: 39114487

订购信息

产品名称产品编号规格价格数量收藏夹

MBP 抗体 (201)

sc-66064
100 µg/ml
CNY2422.00

Can sc-66064: MBP (201) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, sc-66064: MBP (201) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2021-10-28
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Rated 4 out of 5 by Tim Xue from Work in IHCGood staining with low background using IHC in mouse tissue
Date published: 2017-08-28
Rated 5 out of 5 by Johnjiang406 from Pretty good IF results!We tried this MBP antibody in mouse brain cells in vitro. The IF result was pretty good! Will keep working with SCBT antibodies!
Date published: 2016-11-10
Rated 3 out of 5 by JM from Worked well with transfected lysate by WBWorked well with transfected lysate by WB -SCBT QC -SCBT QC
Date published: 2015-02-10
Rated 5 out of 5 by LF from Published IF data with rat skinPublished IF data with rat skin - SCBT Publication Review
Date published: 2013-04-04
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MBP 抗体 (201) is rated 4.3 out of 5 by 4.
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