Date published: 2025-9-7

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MAP-4 抗体 (F-5): sc-271361

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说明书
  • MAP-4抗体(F-5)是小鼠单克隆IgG2b (kappa轻链),规格为200 µg/ml
  • 免疫原氨基酸序列1-300位于human物种的MAP-4的N-terminus
  • 推荐用于 human 来源的MAP-4 isoforms 1 and 2 WB, IP, IF 和 ELISA检测
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP是MAP-4 Antibody (F-5) 适用于 WB 应用。 的首选辅助检测试剂,这些试剂现与MAP-4 Antibody (F-5) 打包提供(请参阅下面的订购信息)。

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    関連項目

    微管是细胞骨架网络的主要组成部分,与称为微管相关蛋白(MAPs)的蛋白质相互作用。微管相关蛋白可分为结构性和动态性两组。MAP蛋白的功能是刺激微管蛋白组装,增强微管稳定性,影响细胞内微管的空间分布,并利用微管极性易位细胞成分。MAP-4是一种非神经元微管相关蛋白,包含三个18个氨基酸重复序列,这些重复序列与其他几种MAP蛋白中的重复序列同源。研究表明,MAP-4与间期微管、有丝分裂纺锤体纤维和有丝分裂运动有关。这种促进微管组装的蛋白质主要在肾、肺、肝、睾丸和脾脏中表达。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    MAP-4 抗体 (F-5) 参考文献:

    1. 小鼠微管相关蛋白 4 (MAP4) 转录物多样性由替代多腺苷酸化产生。  |  Code, RJ. and Olmsted, JB. 1992. Gene. 122: 367-70. PMID: 1487151
    2. 位点特异性微管相关蛋白 4 去磷酸化导致压力过载性心脏肥大中的微管网络致密化。  |  Chinnakkannu, P., et al. 2010. J Biol Chem. 285: 21837-48. PMID: 20436166
    3. 微管相关蛋白 4 与肌动蛋白丝结合并调节其特性。  |  Matsushima, K., et al. 2012. J Biochem. 151: 99-108. PMID: 21937675
    4. 微管相关蛋白(MAP)4 以内在无序的方式与微管相互作用。  |  Hashi, Y., et al. 2014. Biosci Biotechnol Biochem. 78: 1864-70. PMID: 25052097
    5. 微管相关蛋白4是细胞侵袭/迁移的重要调节因子,也是食管鳞状细胞癌的潜在治疗靶细胞。  |  Jiang, YY., et al. 2016. Oncogene. 35: 4846-56. PMID: 26876215
    6. 微管相关蛋白 4 是肺腺癌的预后因子并促进肿瘤进展  |  Xia, X., et al. 2018. Dis Markers. 2018: 8956072. PMID: 29743960
    7. 微管相关蛋白 4 的磷酸化促进缺氧内皮细胞迁移和增殖  |  Zhang, J., et al. 2019. Front Pharmacol. 10: 368. PMID: 31040780
    8. 微管相关蛋白 4 诱导的微管沿肌动蛋白丝伸长有助于细胞突起的形成。  |  Doki, C., et al. 2020. J Biochem. 168: 295-303. PMID: 32289170
    9. 作为心血管疾病新候选者的 MAP4  |  Li, L., et al. 2020. Front Physiol. 11: 1044. PMID: 32982783
    10. 磷脂酰肌醇-3-OH 激酶信号通过微管相关蛋白 4 在内质体隔室中进行空间组织。  |  Thapa, N., et al. 2020. Nat Cell Biol. 22: 1357-1370. PMID: 33139939

    订购信息

    产品名称产品编号规格价格数量收藏夹

    MAP-4 抗体 (F-5)

    sc-271361
    200 µg/ml
    RMB2377.00

    MAP-4 (F-5): m-IgG Fc BP-HRP 套装

    sc-537624
    200 µg Ab; 10 µg BP
    RMB2662.00

    MAP-4 (F-5): m-IgGκ BP-HRP 套装

    sc-534942
    200 µg Ab; 40 µg BP
    RMB2662.00

    What is the recommended fixation method for immunofluorescence staining with MAP-4 (F-5): sc-271361 monoclonal antibody?

    Asked by: TinTin
    Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 5 out of 5 by from Great antibodyThe antibody works very well in 4% PFA fixed cells. I used the dil 1:500 and it worked very well. It also gives a very low background. Great antibody especially for the IF!
    Date published: 2017-12-15
    Rated 5 out of 5 by from Great immunofluorescence cytoplasmic stainingGreat immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
    Date published: 2015-03-12
    Rated 5 out of 5 by from Strong band in WB in HeLa nuclear extractStrong band in WB in HeLa nuclear extract. -SCBT QC
    Date published: 2015-03-12
    Rated 5 out of 5 by from Positive band obtained in WB in HeLa and CakiPositive band obtained in WB in HeLa and Caki-1 whole cell lysates. -SCBT QC
    Date published: 2015-01-07
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    MAP-4 抗体 (F-5) is rated 5.0 out of 5 by 4.
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