Date published: 2025-9-6

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MAGOH 抗体 (21B12): sc-56724

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说明书
  • MAGOH 抗体 21B12 是小鼠单克隆 IgG2a κ,MAGOH抗体, 在8篇文献中引用,规格为200 µg/ml
  • 针对全长MAGOH,human起源
  • MAGOH 抗体 (21B12) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 MAGOH
  • 抗MAGOH抗体(21B12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MAGOH (21B12): sc-56724免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgGκ BP-HRP 是 MAGOH 抗体 (21B12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MAGOH 抗体 (21B12) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

MAGOH抗体(21B12)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学检测小鼠、大鼠和人类来源的MAGOH蛋白。抗MAGOH抗体(21B12)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。MAGOH是果蝇mago nashi的人类同源物,在胚胎发育中起着至关重要的作用,并且在成体组织中普遍表达。MAGOH具有独特的结构,其特征是相邻的两个螺旋结构具有一个极其平坦的六股反平行β片层,这对于其功能至关重要。这种结构配置有利于MAGOH和Y14蛋白之间的相互作用,形成一种复合整体,参与核后处理,包括mRNA的核出口和细胞质定位,以及无义介导的mRNA降解(NMD)监测过程。MAGOH-Y14复合物在细胞质出口后仍保留在mRNA上,确保了外显子-外显子连接复合物-mRNA在剪接连接处的稳定性,证明了前mRNA剪接机制的关键机制。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MAGOH 抗体 (21B12) 参考文献:

  1. MAGOH 与一种新型 RNA 结合蛋白相互作用。  |  Zhao, XF., et al. 2000. Genomics. 63: 145-8. PMID: 10662555
  2. Magoh 是果蝇 mago nashi 蛋白的人类同源物,是剪接依赖性外显子连接复合体的一个组成部分。  |  Kataoka, N., et al. 2001. EMBO J. 20: 6424-33. PMID: 11707413
  3. 外显子连接复合体的 Y14-Magoh 核心结构。  |  Lau, CK., et al. 2003. Curr Biol. 13: 933-41. PMID: 12781131
  4. 剪接增强哺乳动物细胞的翻译:外显子连接复合体的额外功能。  |  Nott, A., et al. 2004. Genes Dev. 18: 210-22. PMID: 14752011
  5. 含 eIF4AIII 的复合物,需要用于 mRNA 定位和无义介导的 mRNA 衰减。  |  Palacios, IM., et al. 2004. Nature. 427: 753-7. PMID: 14973490
  6. 乳腺癌蛋白 MLN51 通过其斑点定域器和 RNA 结合模块与外显子连接复合体缔合作用。  |  Degot, S., et al. 2004. J Biol Chem. 279: 33702-15. PMID: 15166247
  7. 无义介导的 mRNA 降解因子的新功能。  |  Wilkinson, MF. 2005. Trends Genet. 21: 143-8. PMID: 15734573
  8. 在小鼠中生成Magoh条件性等位基因。  |  McMahon, JJ., et al. 2014. Genesis. 52: 752-8. PMID: 24771530
  9. 皮质神经元生成和存活对Magoh的剂量依赖性需求。  |  Sheehan, CJ., et al. 2020. Development. 147: PMID: 31857347
  10. MAGOH与预后不良相关,对低度神经胶质瘤细胞增殖至关重要。  |  Xiao, F., et al. 2023. Aging (Albany NY). 15: 5713-5733. PMID: 37390121
  11. MAGOH通过抑制hnRNPA1表达激活RONΔ160/PI3K/AKT信号通路,促进胃癌的发展。  |  Yu, S., et al. 2024. J Exp Clin Cancer Res. 43: 32. PMID: 38268030
  12. 哺乳动物的 mago nashi 同源物编码一种血清诱导蛋白。  |  Zhao, XF., et al. 1998. Genomics. 47: 319-22. PMID: 9479507

订购信息

产品名称产品编号规格价格数量收藏夹

MAGOH 抗体 (21B12)

sc-56724
200 µg/ml
RMB2377.00

MAGOH (21B12): m-IgG Fc BP-HRP 套装

sc-528619
200 µg Ab; 10 µg BP
RMB2662.00

MAGOH (21B12): m-IgGκ BP-HRP 套装

sc-521043
200 µg Ab, 40 µg BP
RMB2662.00

MAGOH 抗体 (21B12) AC

sc-56724 AC
500 µg/ml, 25% agarose
RMB3129.00

MAGOH 抗体 (21B12) HRP

sc-56724 HRP
200 µg/ml
RMB2377.00

MAGOH 抗体 (21B12) FITC

sc-56724 FITC
200 µg/ml
RMB2482.00

MAGOH 抗体 (21B12) PE

sc-56724 PE
200 µg/ml
RMB2580.00

MAGOH 抗体 (21B12) Alexa Fluor® 488

sc-56724 AF488
200 µg/ml
RMB2685.00

MAGOH 抗体 (21B12) Alexa Fluor® 546

sc-56724 AF546
200 µg/ml
RMB2685.00

MAGOH 抗体 (21B12) Alexa Fluor® 594

sc-56724 AF594
200 µg/ml
RMB2685.00

MAGOH 抗体 (21B12) Alexa Fluor® 647

sc-56724 AF647
200 µg/ml
RMB2685.00

MAGOH 抗体 (21B12) Alexa Fluor® 680

sc-56724 AF680
200 µg/ml
RMB2685.00

MAGOH 抗体 (21B12) Alexa Fluor® 790

sc-56724 AF790
200 µg/ml
RMB2685.00

I am using MAGOH (21B12): sc-56724 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: AbPolly
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from 条带很清晰挺不错的产品,欢迎购买呀。这个蛋白之前试过很多次都没成功。这次一下就检测到了,大家可以试试呀。还有就是这个浓度比较高可以用多次。
Date published: 2020-09-18
Rated 5 out of 5 by from Great anitbody for western blotThe batch of antibody I have worked well on HeLa and K562 total cell extracts for western Blot. The band is specific. The antibody was diluted 500 times into 5% BSA, into TBST and incubated over-night at 4°C with the membrane.
Date published: 2018-01-04
Rated 3 out of 5 by from 染色一般,杂带多哭瞎,没有染出图片的效果,提取的是肿瘤组织,实验室用的是荧光二抗,跟推荐的不同,最终的免疫印迹结果杂带很多,原因待查,
Date published: 2017-09-12
Rated 4 out of 5 by from 用着不错收到抗体有段时间了,在结直肠癌细胞DLD-1细胞中能检测到有明显条带,上样量80ug,100ug都可以。
Date published: 2017-07-11
Rated 5 out of 5 by from Able to publish data for the antibody usedAble to publish data for the antibody used in WB in human PBMCs.
Date published: 2015-06-25
Rated 5 out of 5 by from Able to publish WB data for use in human B cellsAble to publish WB data for use in human B cells.
Date published: 2015-06-25
Rated 5 out of 5 by from CoIP publishable data with HeLa cellsCoIP publishable data with HeLa cells - SCBT Publication Review
Date published: 2015-01-16
Rated 5 out of 5 by from Produced excellent immunoperoxidase nuclearProduced excellent immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human salivary gland tissue. -SCBT QC
Date published: 2014-02-14
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MAGOH 抗体 (21B12) is rated 4.6 out of 5 by 8.
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