Date published: 2025-9-7
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MAGE-A Antibody (6C1) - Western Blotting - Image 361902
MAGE-A 抗体 (6C1): sc-20034. U266 (A) 和 A-375 (B) 全细胞裂解液中 MAGE-A 表达的 Western 印迹分析.
MAGE-A 抗体 (6C1): sc-20034. U266 (A) 和 A-375 (B) 全细胞裂解液中 MAGE-A 表达的 Western 印迹分析.

MAGE-A 抗体 (6C1): sc-20034

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说明书
  • MAGE-A 抗体 6C1 是小鼠单克隆 IgG2a κ，MAGE-A抗体, 在57篇文献中引用，规格为200 µg/ml
  • 针对与全长MAGE-A11相对应的原核重组融合蛋白
  • MAGE-A 抗体 (6C1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 MAGE-A1, MAGE-A2, MAGE-A3, MAGE-A4, MAGE-A6, MAGE-A10 and MAGE-A12
  • 抗MAGE-A抗体（6C1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MAGE-A (6C1): sc-20034的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 MAGE-A 抗体 (6C1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与MAGE-A 抗体 (6C1) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外MAGE抗体，包括MAGE-A, MAGE-A1, MAGE-A11, MAGE-B1, MAGE-C1, MAGE-D2, MAGE-D4和MAGE-D4B

MAGE-A抗体（6C1）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类样本中的MAGE-A蛋白。抗MAGE-A抗体（6C1）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®选项。黑色素瘤相关抗原（MAGE）家族在癌症免疫治疗中发挥着重要作用，因为它们具有独特的表达模式；这些抗原主要出现在肿瘤中，并被细胞毒性T淋巴细胞识别，因此成为很有前景的治疗靶点。MAGE-A蛋白集中在肿瘤细胞核中，表明其参与基因表达调控和细胞周期进展，从而可能影响肿瘤的生长和存活。MAGE基因的表达有限，主要出现在肿瘤中，正常睾丸和胎盘组织除外，这凸显了其作为癌症生物标志物的价值。MAGE-A蛋白的核定位和功能为开发靶向癌症疗法提供了宝贵的见解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MAGE-A 抗体 (6C1) 参考文献:

  1. Mage-b4，一种在生殖细胞分化过程中特异表达的新型黑色素瘤抗原（MAGE）基因。  |  Osterlund, C., et al. 2000. Cancer Res. 60: 1054-61. PMID: 10706124
  2. 细胞溶解性 T 淋巴细胞可识别 HLA-B7 人类肿瘤上的 MAGE-A1 肽。  |  Luiten, R. and van der Bruggen, P. 2000. Tissue Antigens. 55: 149-52. PMID: 10746786
  3. MAGE, GAGE 和 BAGE 基因在人类肝病中的表达：作为肝细胞癌分子标记物的用途。  |  Kobayashi, Y., et al. 2000. J Hepatol. 32: 612-7. PMID: 10782910
  4. 恶性黑色素瘤中抗 MAGE 抗体 57B 的免疫反应：表达频率及与预后参数的相关性。  |  Busam, KJ., et al. 2000. Mod Pathol. 13: 459-65. PMID: 10786815
  5. 转基因树突状细胞和肿瘤细胞作为治疗性癌症疫苗的比较分析。  |  Klein, C., et al. 2000. J Exp Med. 191: 1699-708. PMID: 10811863
  6. 黑色素瘤抗原基因 1 和 2 在人类胃癌和贲门癌中的表达存在差异。  |  Granelli, P., et al. 2000. Scand J Gastroenterol. 35: 528-33. PMID: 10868457
  7. 胆道癌淋巴结微转移的基因检测。  |  Okami, J., et al. 2000. Clin Cancer Res. 6: 2326-32. PMID: 10873083
  8. 原发性和转移性癌症中MAGE-A和NY-ESO-1的表达。  |  Park, TS., et al. 2016. J Immunother. 39: 1-7. PMID: 26641256
  9. 在对 3668 例病例的系统免疫组化分析中，MAGE-A 比 NY-ESO-1 表达更高。  |  Kerkar, SP., et al. 2016. J Immunother. 39: 181-7. PMID: 27070449
  10. 共表达的 MAGE-A 蛋白之间的功能相互作用。  |  Laiseca, JE., et al. 2017. PLoS One. 12: e0178370. PMID: 28542476
  11. MAGE-A抗原作为癌症免疫治疗靶标。  |  Schooten, E., et al. 2018. Cancer Treat Rev. 67: 54-62. PMID: 29763778
  12. MAGE-A家族对胃癌的影响研究进展。  |  Jia, Q., et al. 2023. Zhong Nan Da Xue Xue Bao Yi Xue Ban. 48: 260-267. PMID: 36999473

订购信息

产品名称产品编号规格价格数量收藏夹

MAGE-A 抗体 (6C1)

sc-20034
200 µg/ml
RMB2377.00

MAGE-A (6C1): m-IgGκ BP-HRP 套装

sc-520710
200 µg Ab, 40 µg BP
RMB2662.00

MAGE-A 抗体 (6C1) AC

sc-20034 AC
500 µg/ml, 25% agarose
RMB3129.00

MAGE-A 抗体 (6C1) HRP

sc-20034 HRP
200 µg/ml
RMB2377.00

MAGE-A 抗体 (6C1) FITC

sc-20034 FITC
200 µg/ml
RMB2482.00

MAGE-A 抗体 (6C1) PE

sc-20034 PE
200 µg/ml
RMB2580.00

MAGE-A 抗体 (6C1) Alexa Fluor® 488

sc-20034 AF488
200 µg/ml
RMB2685.00

MAGE-A 抗体 (6C1) Alexa Fluor® 546

sc-20034 AF546
200 µg/ml
RMB2685.00

MAGE-A 抗体 (6C1) Alexa Fluor® 594

sc-20034 AF594
200 µg/ml
RMB2685.00

MAGE-A 抗体 (6C1) Alexa Fluor® 647

sc-20034 AF647
200 µg/ml
RMB2685.00

MAGE-A 抗体 (6C1) Alexa Fluor® 680

sc-20034 AF680
200 µg/ml
RMB2685.00

MAGE-A 抗体 (6C1) Alexa Fluor® 790

sc-20034 AF790
200 µg/ml
RMB2685.00

Is the epitope known for this antibody

Asked by: jackielindi
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2019-05-25

weather can  this antibody (sc-20034) be used to FACS monkey cells？ what's the target peptide of this antibody?

Asked by: scllcs
Thank you for your inquiry. sc-20034 is recommended for detection of MAGE-A1, MAGE-A2, MAGE-A3, MAGE-A4, MAGE-A6, MAGE-A10 and MAGE-A12 of mouse, rat and human origin, so you need to perform sequence analysis or requst the free sample of sc-20034 to do a test. In addition, sc-20034 is raised against a prokaryotic recombinant fusion protein corresponding to the full length MAGE-A11. For more detailed information about this question, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 15
Date published: 2017-12-15

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue? Or even double or triple staining on the same samples?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to phycoerythrin (sc-20034 PE), fluorescein (sc-20034 FITC), Alexa Fluor® 488 (sc-20034 AF488) or Alexa Fluor® 647 (sc-20034 AF647). Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 14
Date published: 2017-01-24
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Rated 5 out of 5 by from MAGE-A IHC-PI used a free sample from this MAGE-A antibody (6C1) and it works really well. We used it 1:50 with citrate (pH 6) antigen retrieval. For the coming experiments, we are buying more of this antibody.
Date published: 2019-03-05
Rated 5 out of 5 by from GreatExcellent IF signal is developed form paraffin embedded human tissue.
Date published: 2018-02-20
Rated 5 out of 5 by from Outstanding product !!Superb for all applications including WB, IF and IHC
Date published: 2017-01-14
Rated 5 out of 5 by from Great IHC(P) dataThe MAGE-A Antibody (6C1): sc-20034 provides excellent staining for IHC with formalin fixed, paraffin-embedded tissue sections. This antibody has also been cited over 10 times for further verification.
Date published: 2017-01-11
Rated 4 out of 5 by from GREAT MONOCLONALI USED THIS ANTIBODY FOR HUMAN CELL LINES AND I OBTAINED A GOOD WB . I RECOMMAND
Date published: 2016-12-16
Rated 4 out of 5 by from Worked well !I tested a sample and it worked perfectly, I recommand
Date published: 2016-12-12
Rated 5 out of 5 by from Great mouse monoclonalGreat mouse monoclonal. We had to switch to this one due to another polyclonal not working very well. WB images were great.
Date published: 2015-10-07
Rated 4 out of 5 by from Good specificity in WB in human transfectedGood specificity in WB in human transfected 293 whole cell lysates. -SCBT QC
Date published: 2015-06-19
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MAGE-A 抗体 (6C1) is rated 4.3 out of 5 by 16.
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