Date published: 2025-9-7
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MafF/G/K Antibody (D-12) - Western Blotting - Image 108802
MafF/G/K 抗体 (D-12): sc-166548. 非转染: sc-117752 (A) 和小鼠 MafG 转染: sc-125575 (B) 293T 全细胞裂解液中 MafG 表达的 Western 印迹分析.
MafF/G/K 抗体 (D-12): sc-166548. 非转染: sc-117752 (A) 和小鼠 MafG 转染: sc-125575 (B) 293T 全细胞裂解液中 MafG 表达的 Western 印迹分析.

MafF/G/K 抗体 (D-12): sc-166548

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说明书
  • MafF/G/K 抗体 D-12 是小鼠单克隆 IgG1 κ，MafF/G/K抗体, 在8篇文献中引用，规格为200 µg/ml
  • 免疫human物种的MafF/G/K的氨基酸63-126
  • MafF/G/K 抗体 (D-12) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 MafF, MafG and NF-E2 p18 (also designated MafK)
  • 抗MafF/G/K抗体（D-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于ChIP的TransCruz试剂(sc-166548 X, 200 µg/0.1 ml)
  • 关于如何获取MafF/G/K (D-12): sc-166548的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是MafF/G/K Antibody (D-12) for WB applications. 的首选辅助检测试剂，这些试剂现与MafF/G/K Antibody (D-12) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加Maf抗体，包括MafA, MafB, MafF/G/K, MafG和MafK

MafF/G/K抗体（D-12）是一种IgG1 κ小鼠单克隆MafF/G/K抗体（也称为MafF/G/K抗体），可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的MafF/G/K蛋白。MafF/G/K抗体（D-12）既有非偶联的抗MafF/G/K抗体形式，也有多种偶联形式的抗MafF/G/K抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Maf家族成员属于碱性区域/亮氨酸拉链（bZIP）转录因子，根据特定的蛋白伴侣和目标启动子的环境，它们以正性或负性方式影响转录。c-Maf（Maf-2）和密切相关的家族成员神经视网膜亮氨酸拉链（Nrl）、L-Maf和Krml1/MafB（Maf-1）都与T-MARE位点结合，并涉及多种发育和生理作用。三种小型Maf家族蛋白MafF、MafG和MafK是NF-E2的组成部分，它们与NF-E2的大型组织限制性亚基p45形成异二聚体，并进一步参与许多红细胞特异性基因的转录调控。MafG普遍表达，在VMS、心脏和骨骼肌中表达最高；其表达在应激反应中被诱导。MafK，也称为NF-E2 p18，主要在间充质和造血细胞以及神经元的发育过程中表达。MafK与NF-E2和各种CNC蛋白形成异二聚体。MafF在肺中表达最丰富，被认为可以补偿MafG和MafK的功能丧失突变。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MafF/G/K 抗体 (D-12) 参考文献:

  1. Maf 基因对小鼠晶状体纤维细胞发育和分化的调控  |  Ring, BZ., et al. 2000. Development. 127: 307-17. PMID: 10603348
  2. 亲电子反应元件激活剂对 mafF、mafG 和 mafK 表达的不同诱导。  |  Moran, JA., et al. 2002. Biochem J. 361: 371-7. PMID: 11772409
  3. 新型人类基因 MIP 是 hMafF 的共激活因子。  |  Ye, X., et al. 2006. Arch Biochem Biophys. 449: 87-93. PMID: 16549056
  4. 主动转录抑制需要 MafG sumoylation。  |  Motohashi, H., et al. 2006. Mol Cell Biol. 26: 4652-63. PMID: 16738329
  5. 胰腺β细胞过表达 MafK 会导致葡萄糖刺激的胰岛素分泌受损。  |  Shimohata, H., et al. 2006. Biochem Biophys Res Commun. 346: 671-80. PMID: 16780794
  6. 小型 Maf 蛋白（MafF、MafG、MafK）：历史、结构和功能。  |  Katsuoka, F. and Yamamoto, M. 2016. Gene. 586: 197-205. PMID: 27058431
  7. 无处不在的转录因子 MafK 的条件表达可诱导红白血病细胞分化。  |  Igarashi, K., et al. 1995. Proc Natl Acad Sci U S A. 92: 7445-9. PMID: 7638211
  8. 小 Maf 蛋白与 Fos 异源二聚体，可能是 NF-E2 转录因子的竞争性抑制因子。  |  Kataoka, K., et al. 1995. Mol Cell Biol. 15: 2180-90. PMID: 7891713
  9. Maf/Nrl 家族蛋白识别扩展 DNA 结合位点需要邻近基本结构域的保守区域。  |  Kerppola, TK. and Curran, T. 1994. Oncogene. 9: 3149-58. PMID: 7936637
  10. 小 Maf 蛋白与人类转录因子 TCF11/Nrf1/LCR-F1 相互作用。  |  Johnsen, O., et al. 1996. Nucleic Acids Res. 24: 4289-97. PMID: 8932385
  11. c-Maf 与 c-Myb 相互作用，在分化过程中调节早期髓系基因的转录。  |  Hedge, SP., et al. 1998. Mol Cell Biol. 18: 2729-37. PMID: 9566892
  12. 大鼠 maf 相关因子：DNA 结合和异二聚体形成的特异性。  |  Matsushima-Hibiya, Y., et al. 1998. Biochem Biophys Res Commun. 245: 412-8. PMID: 9571165

订购信息

产品名称产品编号规格价格数量收藏夹

MafF/G/K 抗体 (D-12)

sc-166548
200 µg/ml
RMB2377.00

MafF/G/K (D-12): m-IgG Fc BP-HRP 套装

sc-527168
200 µg Ab; 10 µg BP
RMB2662.00

MafF/G/K (D-12): m-IgG1 BP-HRP 套装

sc-532541
200 µg Ab; 20 µg BP
RMB2662.00

MafF/G/K 抗体 (D-12) X

sc-166548 X
200 µg/0.1 ml
RMB2377.00

MafF/G/K 抗体 (D-12) AC

sc-166548 AC
500 µg/ml, 25% agarose
RMB3129.00

MafF/G/K 抗体 (D-12) HRP

sc-166548 HRP
200 µg/ml
RMB2377.00

MafF/G/K 抗体 (D-12) FITC

sc-166548 FITC
200 µg/ml
RMB2482.00

MafF/G/K 抗体 (D-12) PE

sc-166548 PE
200 µg/ml
RMB2580.00

MafF/G/K 抗体 (D-12) Alexa Fluor® 488

sc-166548 AF488
200 µg/ml
RMB2685.00

MafF/G/K 抗体 (D-12) Alexa Fluor® 546

sc-166548 AF546
200 µg/ml
RMB2685.00

MafF/G/K 抗体 (D-12) Alexa Fluor® 594

sc-166548 AF594
200 µg/ml
RMB2685.00

MafF/G/K 抗体 (D-12) Alexa Fluor® 647

sc-166548 AF647
200 µg/ml
RMB2685.00

MafF/G/K 抗体 (D-12) Alexa Fluor® 680

sc-166548 AF680
200 µg/ml
RMB2685.00

MafF/G/K 抗体 (D-12) Alexa Fluor® 790

sc-166548 AF790
200 µg/ml
RMB2685.00

Is this a new version of anti-MafK antibody (C-18)? I need an antibody antiMafK and CHIP grade. Does this antibody work for my purpose?

Asked by: flyingcat
Thank you for your question. This mouse monoclonal antibody, MafF/G/K (D-12): sc-166548, is currently the suggested replacement antibody for the now discontinued goat polyclonal (C-18) antibody. This is not raised against the exact same antigen. We have not yet tested this antibody for use in ChIP, and we are not aware of any publications citing use of the antibody for ChIP. For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to test the antibody before placing your order. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-10-25

When should the TransCruz reagent sc-166548 X be used instead of the standard form sc-166548?

Asked by: Germaine
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 2 out of 5 by from Single band, not at appropriate molecular weightIn ARPE-19 cells, nice, clear band detected at ~70 kDa, far from predicted molecular weight of ~20 kDa. 40 ug RIPA-extracted lysates.
Date published: 2017-03-07
Rated 5 out of 5 by from Great Antibody for WBThis antibody is well-cited for a variety of applications including WB, IP, IF, and even ELISA. Strong, clear signal with little to no background. Works well in a variety of species including mouse, rat, and human samples.
Date published: 2017-01-27
Rated 3 out of 5 by from WBNO signal has been detected in human decidua stromal cells and trophoblast cells by WB and IHC-P.
Date published: 2017-01-07
Rated 5 out of 5 by from Works great!I used this for my human samples and it works great!
Date published: 2016-11-04
Rated 4 out of 5 by from Worked well with transfected lysate by WBWorked well with transfected lysate by WB -SCBT QC
Date published: 2015-03-10
Rated 5 out of 5 by from Produced excellent immunofluorescence nuclearProduced excellent immunofluorescence nuclear staining in methanol-fixed NIH/3T3 cells. -SCBT QC
Date published: 2015-01-26
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MafF/G/K 抗体 (D-12) is rated 4.0 out of 5 by 6.
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