Date published: 2025-9-8
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MAFbx Antibody (F-9) - Immunofluorescence - Image 295293
MAFbx 抗体 (F-9): sc-166806. 福尔马林固定的 Hep G2 细胞免疫荧光染色, 显示胞质定位.
MAFbx 抗体 (F-9): sc-166806. 福尔马林固定的 Hep G2 细胞免疫荧光染色, 显示胞质定位.

MAFbx 抗体 (F-9): sc-166806

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说明书
  • MAFbx 抗体 F-9 是小鼠单克隆 IgG1 κ，MAFbx抗体, 在89篇文献中引用，规格为200 µg/ml
  • 针对氨基酸1-300的抗原，在MAFbx的N-terminus处映射，human来源
  • MAFbx 抗体 (F-9) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 MAFbx isoforms 1-3 and FBXO25 isoforms 1 and 2
  • 抗MAFbx抗体（F-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MAFbx (F-9): sc-166806的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是MAFbx Antibody (F-9) for WB applications. 的首选辅助检测试剂，这些试剂现与MAFbx Antibody (F-9) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

MAFbx抗体（F-9）是一种IgG1κ小鼠单克隆MAFbx抗体（也称为肌肉萎缩F-box蛋白抗体、MAFbx抗体、ATROGIN1抗体、Atrogin-1抗体、F-box蛋白32抗体、Fbx32抗体、仅F-box蛋白32抗体或FBXO-32抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的MAFbx蛋白。MAFbx抗体（F-9）有非偶联抗MAFbx抗体形式和多种偶联形式的抗MAFbx抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。肌肉萎缩F-box（MAFbx）是一种E3泛素连接酶，可启动ATP依赖的泛素介导的蛋白质水解并促进肌肉萎缩。MAFbx转录物在经历萎缩的心脏和骨骼肌中含量丰富。MAFbx -/-小鼠对肌肉萎缩有抵抗力。MAFbx被认为能够识别并结合一些磷酸化蛋白质，并在骨骼肌萎缩期间促进它们的泛素化和降解。它通过转录辅激活因子中的序列通过泛素化与MyoD相互作用，因此可能通过确定MyoD的丰度在肌肉分化过程中发挥重要作用。MAFbx在心脏和骨骼肌中特异性表达。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MAFbx 抗体 (F-9) 参考文献:

  1. 鉴定骨骼肌萎缩所需的泛素连接酶  |  Bodine, SC., et al. 2001. Science. 294: 1704-8. PMID: 11679633
  2. 介导骨骼肌肥大和萎缩的信号通路。  |  Glass, DJ. 2003. Nat Cell Biol. 5: 87-90. PMID: 12563267
  3. 过氧化合物刺激骨骼肌肌管中泛素结合活性和特定 E2 和 E3 蛋白基因的表达。  |  Li, YP., et al. 2003. Am J Physiol Cell Physiol. 285: C806-12. PMID: 12773310
  4. 空腹和糖尿病期间胰岛素样生长因子 I 下降在诱导 atrogin-1/MAFbx 中的作用。  |  Dehoux, M., et al. 2004. Endocrinology. 145: 4806-12. PMID: 15284206
  5. 冬眠的金绒毛地松鼠（Spermophilus lateralis）肌球蛋白同工酶表达和 MAFbx mRNA 水平。  |  Rourke, BC., et al. 2004. Physiol Biochem Zool. 77: 582-93. PMID: 15449229
  6. 由 SCF (MAFbx) 泛素连接酶介导的 MyoD 降解。  |  Tintignac, LA., et al. 2005. J Biol Chem. 280: 2847-56. PMID: 15531760
  7. MAFbx/atrogin-1和MuRF1在骨骼肌萎缩中的作用和调控。  |  Foletta, VC., et al. 2011. Pflugers Arch. 461: 325-35. PMID: 21221630
  8. MAFbx/Atrogin-1是无负荷心脏萎缩性重塑所必需的。  |  Baskin, KK., et al. 2014. J Mol Cell Cardiol. 72: 168-76. PMID: 24650875
  9. 骨骼肌萎缩与 E3 泛素连接酶 MuRF1 和 MAFbx/atrogin-1。  |  Bodine, SC. and Baehr, LM. 2014. Am J Physiol Endocrinol Metab. 307: E469-84. PMID: 25096180
  10. E3 泛素连接酶 MuRF-1 和 MAFbx 在骨骼肌质量丧失中的作用。  |  Rom, O. and Reznick, AZ. 2016. Free Radic Biol Med. 98: 218-230. PMID: 26738803
  11. 大鼠比目鱼肌在后肢负重情况下Atrogin-1/MAFbx mRNA的表达受组蛋白去乙酰化酶1的调控  |  Mochalova, EP., et al. 2019. Sci Rep. 9: 10263. PMID: 31311969
  12. 系统综述：大鼠和小鼠MTOR、MURF-1和MAFBX基因表达变化模型  |  de Macêdo, MRC., et al. 2020. Crit Rev Eukaryot Gene Expr. 30: 57-75. PMID: 32421985

订购信息

产品名称产品编号规格价格数量收藏夹

MAFbx 抗体 (F-9)

sc-166806
200 µg/ml
RMB2377.00

MAFbx (F-9): m-IgG Fc BP-HRP 套装

sc-527189
200 µg Ab; 10 µg BP
RMB2662.00

MAFbx (F-9): m-IgG1 BP-HRP 套装

sc-532562
200 µg Ab; 20 µg BP
RMB2662.00

MAFbx 抗体 (F-9) AC

sc-166806 AC
500 µg/ml, 25% agarose
RMB3129.00

MAFbx 抗体 (F-9) HRP

sc-166806 HRP
200 µg/ml
RMB2377.00

MAFbx 抗体 (F-9) FITC

sc-166806 FITC
200 µg/ml
RMB2482.00

MAFbx 抗体 (F-9) PE

sc-166806 PE
200 µg/ml
RMB2580.00

MAFbx 抗体 (F-9) Alexa Fluor® 488

sc-166806 AF488
200 µg/ml
RMB2685.00

MAFbx 抗体 (F-9) Alexa Fluor® 546

sc-166806 AF546
200 µg/ml
RMB2685.00

MAFbx 抗体 (F-9) Alexa Fluor® 594

sc-166806 AF594
200 µg/ml
RMB2685.00

MAFbx 抗体 (F-9) Alexa Fluor® 647

sc-166806 AF647
200 µg/ml
RMB2685.00

MAFbx 抗体 (F-9) Alexa Fluor® 680

sc-166806 AF680
200 µg/ml
RMB2685.00

MAFbx 抗体 (F-9) Alexa Fluor® 790

sc-166806 AF790
200 µg/ml
RMB2685.00

ご担当者様 sc-166806, sc-398608の二点を購入検討しております。下記につきましてご教示いただけますと幸いです。 ・日本語SDSの提供が可能か ・カルタヘナ法該当有無、ヒト検体由来原料やヒト細胞抽出成分の有無 よろしくお願いいたします。

Asked by: Anonymous
ご質問ありがとうございます。申し訳ございませんが、抗体のSDSは提供していません。詳しくは日本のディーラーにお尋ねください。宜しくお願い致します。 Thank you for your question. We are sorry, but we do not provide SDS for antibodies. Please ask your Japanese dealer for more information. Thank you for your cooperation.
Answered by: Jiajun Z
Date published: 2024-07-26

I blocked membrane with 5% BSA and also diluted the antibody MAFbx Antibody (F-9): sc-166806 HRP in BSA but there was no detection. What should I do?

Asked by: nitrogenlinh
Thank you for your question. We recommend 5%milk or https://www.scbt.com/p/blotto-non-fat-dry-milk. Here is our protocol :https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: BlakeJ
Date published: 2024-04-15

检测到35-40KD中间有一条条带 以及50-55KD中间有一条条带 不知道是哪个条带是MAFbx

Asked by: Anonymous
感谢您的提问。请联系我们亚洲技术服务部门，了解详情。邮箱：[email protected], 电话：021-60936350, 或者官网在线聊天都可以联系到我们。 Thank you for your inquiry. Please contact our Asian Technical Service Department to get the details. We are available at [email protected], 021-60936350, or by live chat on our website.
Answered by: Technical Support 12
Date published: 2021-10-18

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: cjMara
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 12
Date published: 2017-01-24
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Rated 5 out of 5 by from Well used for western blotThis antibody is great for western blot of gastrocnemius (GA) muscle.
Date published: 2025-04-02
Rated 4 out of 5 by from GoodGood signal in both cells and tissue. Correct at predicted MW.
Date published: 2024-11-09
Rated 5 out of 5 by from 不错的产品东西很好，唯一不足的就是特异性有点缺陷。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。。
Date published: 2018-05-01
Rated 5 out of 5 by from work well for wb1:250 diluted for wb of rat protein,the band is strong and the background is clear.
Date published: 2017-10-07
Rated 5 out of 5 by from GreatC2C12 cell line (mouse), western blot, loading 20ug, got single band at right size.
Date published: 2017-09-19
Rated 4 out of 5 by from It worked2 bands between 30-50 kDa were detected. The membrane was clear of background. 1:1000 conc.
Date published: 2017-08-31
Rated 5 out of 5 by from Fantastic antibody!This antibody is great for detection of endogenous mouse protein in atrophied muscle samples. I used it for Western blotting at 1:1000 and got a very clean blot.
Date published: 2017-08-02
Rated 5 out of 5 by from Strong product !!Nice western data;strong signal with low background; also strong IF staining
Date published: 2017-01-30
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MAFbx 抗体 (F-9) is rated 4.8 out of 5 by 12.
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