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mAChR M1 Antibody (G-9) - Western Blotting - Image 145692 — mAChR M1 抗体 (G-9): sc-365966。非转染：sc-117752 (A) 和人 mAChR M1 转染：sc-158705 (B) 293T 全细胞裂解液中 mAChR M1 表达的 Western 印迹分析。

mAChR M1 Antibody (G-9) - Western Blotting - Image 401524 — mAChR M1 抗体 (G-9) HRP: sc-365966 HRP. 直接 Western 印迹分析 SK-BR-3 全细胞裂解液中 mAChR M1 的表达.

mAChR M1 Antibody (G-9) - Western Blotting - Image 362378 — mAChR M1 抗体 (G-9): sc-365966. SK-BR-3 全细胞裂解液中 mAChR M1 表达的 Western 印迹分析.

mAChR M1 抗体 (G-9): sc-365966。非转染：sc-117752 (A) 和人 mAChR M1 转染：sc-158705 (B) 293T 全细胞裂解液中 mAChR M1 表达的 Western 印迹分析。

mAChR M1 抗体 (G-9): sc-365966

Name: mAChR M1抗体 (G-9)
Brand: SCBIO
SKU: sc-365966
Price: 2377.0 CNY
Availability: InStock

5.0(2)

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说明书

mAChR M1 抗体 G-9 是小鼠单克隆 IgG₁ κ，mAChR M1抗体, 在14篇文献中引用，规格为200 µg/ml
免疫human物种的mAChR M1的氨基酸231-350
mAChR M1 抗体 (G-9) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 mAChR M1
抗mAChR M1抗体（G-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
关于如何获取mAChR M1 (G-9): sc-365966的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG₁ BP-HRP是mAChR M1 Antibody (G-9) for WB applications. 的首选辅助检测试剂，这些试剂现与mAChR M1 Antibody (G-9) 打包提供（请参阅下面的订购信息）。

查看产品引用文献(14)

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mAChR M1 抗体 (G-9) 参考文献:

毒蕈碱乙酰胆碱受体：结构与功能。 | Hulme, EC., et al. 1991. Biochem Soc Trans. 19: 133-8. PMID: 1903735
毒蕈碱乙酰胆碱受体：典型的 G 偶联受体。 | Hulme, EC. 1990. Symp Soc Exp Biol. 44: 39-54. PMID: 2130518
第五种毒蕈碱乙酰胆碱受体的分子克隆和表达。 | Liao, CF., et al. 1989. J Biol Chem. 264: 7328-37. PMID: 2540186
M2 muscarinic 受体的一级结构和生化特性。 | Peralta, EG., et al. 1987. Science. 236: 600-5. PMID: 3107123
阿尔茨海默氏症、帕金森氏症或额叶痴呆症患者海马体和颞叶皮层中胆碱能受体 Muscarinic 1 (CHRM1) 蛋白的缺失：对患者存活的影响》（Loss of Cholinergic Receptor Muscarinic 1 (CHRM1) Protein of Hippocampus and Temporal Cortex of a Subset of Individual with Alzheimer's Disease, Parkinson's Disease, or Frontotemporal Dementia: Implications for Patient Survival. | Sabbir, MG., et al. 2022. J Alzheimers Dis. 90: 727-747. PMID: 36155524
卵巢过度刺激诱导大鼠卵巢胆碱能受体毒蕈碱 1 活性的研究 | Şanlı, C., et al. 2023. Turk J Obstet Gynecol. 20: 53-58. PMID: 36908094
小鼠海马区与皮层胆碱能受体毒蕈碱 1 的缺失会对呼吸链相关蛋白的翻译后修饰和超分子组装、线粒体超微结构和呼吸产生不同影响：对阿尔茨海默病的影响。 | Sabbir, MG., et al. 2023. Front Cell Dev Biol. 11: 1179252. PMID: 37293125
胆碱能受体 Muscarinic 1 在培养的背根神经节神经元中与线粒体共定位，其缺失会破坏外周神经元的线粒体超微结构：对阿尔茨海默病的影响 | Sabbir, MG. 2024. J Alzheimers Dis. 98: 247-264. PMID: 38427478
毒蕈碱受体--特征, 耦合和功能。 | Caulfield, MP. 1993. Pharmacol Ther. 58: 319-79. PMID: 7504306
毒蕈碱乙酰胆碱受体亚型：定位和结构/功能。 | Brann, MR., et al. 1993. Prog Brain Res. 98: 121-7. PMID: 8248499
从出生后到老年期大鼠大脑中毒蕈碱受体亚型的分布。 | Tice, MA., et al. 1996. Brain Res Dev Brain Res. 92: 70-6. PMID: 8861724
毒蕈碱乙酰胆碱受体亚型（m1-m5）的药理学：哺乳动物细胞中的高通量测定。 | Bräuner-Osborne, H. and Brann, MR. 1996. Eur J Pharmacol. 295: 93-102. PMID: 8925880

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
mAChR M1 抗体 (G-9)	sc-365966	200 µg/ml	RMB2377.00
mAChR M1 (G-9): m-IgG Fc BP-HRP 套装	sc-527403	200 µg Ab; 10 µg BP	RMB2662.00
mAChR M1 (G-9): m-IgG₁ BP-HRP 套装	sc-532776	200 µg Ab; 20 µg BP	RMB2662.00
mAChR M1 抗体 (G-9) AC	sc-365966 AC	500 µg/ml, 25% agarose	RMB3129.00
mAChR M1 抗体 (G-9) HRP	sc-365966 HRP	200 µg/ml	RMB2377.00
mAChR M1 抗体 (G-9) FITC	sc-365966 FITC	200 µg/ml	RMB2482.00
mAChR M1 抗体 (G-9) PE	sc-365966 PE	200 µg/ml	RMB2580.00
mAChR M1 抗体 (G-9) Alexa Fluor^® 488	sc-365966 AF488	200 µg/ml	RMB2685.00
mAChR M1 抗体 (G-9) Alexa Fluor^® 546	sc-365966 AF546	200 µg/ml	RMB2685.00
mAChR M1 抗体 (G-9) Alexa Fluor^® 594	sc-365966 AF594	200 µg/ml	RMB2685.00
mAChR M1 抗体 (G-9) Alexa Fluor^® 647	sc-365966 AF647	200 µg/ml	RMB2685.00
mAChR M1 抗体 (G-9) Alexa Fluor^® 680	sc-365966 AF680	200 µg/ml	RMB2685.00
mAChR M1 抗体 (G-9) Alexa Fluor^® 790	sc-365966 AF790	200 µg/ml	RMB2685.00

Dear SCBT Team, Would it be possible to recieve from you free sample of th efollowing antibody: sc-365966 ?? With best regards, Michał

Asked by: Michał

Thank you for your question. Please contact Technical Service or your local distributor for information on how to receive a free 10 ug sample of this monoclonal antibody.

Answered by: Tech Service

Date published: 2020-03-02

Does this antibody also work with mouse brain extracts? I could not find neither an example western blot nor a paper reference with this antibody with mouse brain samples? we would like to use it with hippocampus lysates in a western blot..

Asked by: Yunus Emre

Thank you for your question. We currently have no data on the reactivity of this antibody in mouse brain extracts in western blot. However, our one-year warranty covers this application, so if it does not work as expected please contact our Technical Service by phone, email or by Live Chat and if we cannot resolve the issue, we will provide an appropriate replacement free of charge.

Answered by: SCBT Heidelberg Support

Date published: 2018-12-14

I need to use an antibody to detect the protein in samples of bovine origin. Do you know if sc-365966: mAChR M1 (G-9) mouse monoclonal antibody will work?

Asked by: jerojero

Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.

Answered by: Technical Support

Date published: 2017-03-06

Rated 5 out of 5 by Sabbir from The best Chrm1/M1R/M1 antibody ever seen on earthThis one is my favorite antibody. Allow me to explain why!. Muscarinic receptors have 5 suptypes (1-5) with conserved sequence homology, so making a subtype specific antibody will require to choose the epitope carefully and that depends on experience, knowledge and expertise. Previously, I have been FRUSTRATED with costly M1R antibody purchased from Alomone labs Ltd (cat: AMR-001) that cost >700$ a vial. It was simply a garbage antibody and spend tons of time to figure out whether it was the antibody or my technique/nature of tissue etc. THEN I GOT SCBT (SC365966) ANTIBODY, and I am so satisfied. The antibody is highly subtype specific, see the attached bot, in which I cloned and expressed GFP-tagged 5 mAChr subtypes and validated the M1R subtype specificity by anti-GFP antibody (use 1:1000 dilution). In addition this antibody is superb for Immunoprecipitation.

Date published: 2017-02-01

Rated 5 out of 5 by MS from Nice Western blot data of mAChR M1 expressionNice Western blot data of mAChR M1 expression in non-transfected and human mAChR M1 transfected 293 whole cell lysates. -SCBT QC

Date published: 2014-06-29

mAChR M1抗体 (G-9) is rated 5.0 out of 5 by 2.

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种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	CHRM1	Entrez Gene ID: 1128	11q12.3	XM_011544742, NM_000738	UniProt ID: P11229	118510
Mouse	Chrm1	Entrez Gene ID: 12669	19 A	NM_001112697, NM_007698	UniProt ID: P12657	N/A

mAChR M1 抗体 (G-9): sc-365966

mAChR M1 抗体 (G-9) 参考文献:

mAChR M1 抗体 (G-9) 规格

替代产品名称: CHRM1

产品克隆名称: G-9

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG₁ kappa light chain

氨基酸: 231-350

抗原表位靶标: mAChR M1

抗原表位种属: human

产品反应性/检测: mAChR M1

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 14篇各种科学出版物的引文

mAChR M1 抗体 (G-9)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin