Date published: 2025-9-11
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M13 Major Coat Protein Antibody (RL-ph1) - Western Blotting - Image 384054
M13 主要包被蛋白抗体 (RL-ph1) HRP: sc-53004 HRP. 直接 Western 印迹分析 M13 噬菌体裂解液中 M13 主要外壳蛋白的表达.
M13 Major Coat Protein Antibody (RL-ph1) - Western Blotting - Image 384054
M13 主要包被蛋白抗体 (RL-ph1) HRP: sc-53004 HRP. 直接 Western 印迹分析 M13 噬菌体裂解液中 M13 主要外壳蛋白的表达.
M13 Major Coat Protein Antibody (RL-ph1) - Western Blotting - Image 137421
M13 主要包被蛋白抗体 (RL-ph1): sc-53004. Western 印迹分析 M13 噬菌体裂解液中 M13 主要外壳蛋白的表达.
M13 Major Coat Protein Antibody (RL-ph1) - Western Blotting - Image 393095
M13 主要包被蛋白抗体 (RL-ph1) HRP: sc-53004 HRP. 直接 Western 印迹分析 M13 噬菌体全细胞裂解液中 M13 主要外壳蛋白的表达.
M13 主要包被蛋白抗体 (RL-ph1) HRP: sc-53004 HRP. 直接 Western 印迹分析 M13 噬菌体裂解液中 M13 主要外壳蛋白的表达.

M13 Major Coat Protein 抗体 (RL-ph1): sc-53004

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说明书
  • M13 Major Coat Protein 抗体 RL-ph1 是小鼠单克隆 IgG2b κ，M13 Major Coat Protein抗体, 在15篇文献中引用，规格为200 µg/ml
  • 针对分离的M13噬菌体衣壳蛋白
  • 建议使用M13 Major Coat Protein抗体（RL-ph1）通过WB, IP, IF和FCM检测M13
  • 抗M13 Major Coat Protein抗体（RL-ph1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取M13 Major Coat Protein (RL-ph1): sc-53004的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2b BP-HRP">m-IgG2b BP-HRP是M13 Major Coat Protein Antibody (RL-ph1) 适用于 WB 应用。 的首选辅助检测试剂，这些试剂现与M13 Major Coat Protein Antibody (RL-ph1) 打包提供（请参阅下面的订购信息）。

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研究信息

関連項目

额外M13 Bacteriophage抗体，包括M13 Major Coat Protein和M13 Minor Coat Protein

M13 主要衣壳蛋白抗体（RL-ph1）是一种鼠单克隆IgG2b kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和流式细胞术（FCM）检测M13来源的M13主要衣壳蛋白。M13 Major Coat Protein (RL-ph1) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合型。M13 Major Coat Protein 在丝状噬菌体形态发生中起着至关重要的作用，因为这种蛋白质对于病毒组装至关重要。M13主要衣壳蛋白以前体形式合成，并经历一系列修饰，包括插入宿主细胞质膜，随后裂解为成熟形式。成熟衣壳蛋白随后组装成病毒结构，当病毒DNA穿过细胞质膜时，大约有2400个拷贝被整合进来。M13主要衣壳蛋白的氨基末端包含一个暴露于大肠杆菌细胞外部的抗原位点，有助于进行噬菌体感染所需的相互作用。最初的合成包括一个23个氨基酸的氨基末端"引导肽"，被称为"前衣壳"，在体内作为生物合成的前体。了解M13主要衣壳蛋白的结构和功能对于阐明噬菌体组装和感染机制至关重要，这使得抗M13主要衣壳蛋白抗体（RL-ph1）成为研究噬菌体生物学以及在分子生物学和生物技术中的应用的研究人员的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

M13 Major Coat Protein 抗体 (RL-ph1) 参考文献:

  1. 噬菌体 M13 主要外壳蛋白的蛋白-脂质相互作用。  |  Stopar, D., et al. 2003. Biochim Biophys Acta. 1611: 5-15. PMID: 12659940
  2. 受病毒感染的大肠杆菌提取物中 M13 噬菌体的膜相关组装。  |  Wickner, W. and Killick, T. 1977. Proc Natl Acad Sci U S A. 74: 505-9. PMID: 15248
  3. M13 主要衣壳蛋白的膜组装：铰链区和倾斜跨膜结构域结构适应性的证据。  |  Spruijt, RB., et al. 2004. Biochemistry. 43: 13972-80. PMID: 15518546
  4. M13 主要衣壳蛋白的膜结合构象：通过 FRET 衍生约束进行结构验证。  |  Vos, WL., et al. 2005. J Biol Chem. 280: 38522-7. PMID: 16150733
  5. 膜嵌入式 M13 主要衣壳蛋白的结构对疏水压力不敏感。  |  Vos, WL., et al. 2007. Biophys J. 93: 3541-7. PMID: 17704180
  6. 整体膜蛋白的可溶性前体：噬菌体 M13 感染大肠杆菌后原膜蛋白的合成。  |  Ito, K., et al. 1979. Proc Natl Acad Sci U S A. 76: 1199-203. PMID: 375229
  7. 信号肽酶裂解位点下游的突变会影响噬菌体外壳蛋白的裂解，但不会影响膜插入。  |  Russel, M. and Model, P. 1981. Proc Natl Acad Sci U S A. 78: 1717-21. PMID: 7015343
  8. 噬菌体 IKe 和 M13 主要外壳蛋白突变体中 Cys 残基的可及性和动态性。  |  Khan, AR., et al. 1995. Biochemistry. 34: 12388-97. PMID: 7547983
  9. 噬菌体 M13 衣壳蛋白在大肠杆菌细胞质膜和合成脂质囊泡中的不对称取向。  |  Wickner, W. 1976. Proc Natl Acad Sci U S A. 73: 1159-63. PMID: 772680
  10. M13 主要衣壳蛋白在不同膜模拟系统中的局部动态。  |  Stopar, D., et al. 1996. Biochemistry. 35: 15467-73. PMID: 8952500
  11. M13 主要外壳蛋白在去垢胶束中的溶液结构：涉及特定残基的噬菌体组装模型的基础。  |  Papavoine, CH., et al. 1998. J Mol Biol. 282: 401-19. PMID: 9735296
  12. 噬菌体显示特异性细胞骨架抗原抗体。用单克隆抗体竞争性洗脱进行筛选。  |  Meulemans, EV., et al. 1995. Hum Antibodies Hybridomas. 6: 113-8. PMID: 8597626

订购信息

产品名称产品编号规格价格数量收藏夹

M13 Major Coat Protein 抗体 (RL-ph1)

sc-53004
200 µg/ml
RMB2377.00

M13 Major Coat Protein (RL-ph1): m-IgG Fc BP-HRP 套装

sc-525568
200 µg Ab; 10 µg BP
RMB2662.00

M13 Major Coat Protein (RL-ph1): m-IgG2b BP-HRP 套装

sc-549584
200 µg Ab; 10 µg BP
RMB2662.00

M13 Major Coat Protein 抗体 (RL-ph1) AC

sc-53004 AC
500 µg/ml, 25% agarose
RMB3129.00

M13 Major Coat Protein 抗体 (RL-ph1) HRP

sc-53004 HRP
200 µg/ml
RMB2377.00

M13 Major Coat Protein 抗体 (RL-ph1) FITC

sc-53004 FITC
200 µg/ml
RMB2482.00

M13 Major Coat Protein 抗体 (RL-ph1) PE

sc-53004 PE
200 µg/ml
RMB2580.00

M13 Major Coat Protein 抗体 (RL-ph1) Alexa Fluor® 488

sc-53004 AF488
200 µg/ml
RMB2685.00

M13 Major Coat Protein 抗体 (RL-ph1) Alexa Fluor® 546

sc-53004 AF546
200 µg/ml
RMB2685.00

M13 Major Coat Protein 抗体 (RL-ph1) Alexa Fluor® 594

sc-53004 AF594
200 µg/ml
RMB2685.00

M13 Major Coat Protein 抗体 (RL-ph1) Alexa Fluor® 647

sc-53004 AF647
200 µg/ml
RMB2685.00

M13 Major Coat Protein 抗体 (RL-ph1) Alexa Fluor® 680

sc-53004 AF680
200 µg/ml
RMB2685.00

M13 Major Coat Protein 抗体 (RL-ph1) Alexa Fluor® 790

sc-53004 AF790
200 µg/ml
RMB2685.00

Dear Sir/Madam Can you please indicate the main differences between sc-53004 and sc-53005, please? Apart from IgG isotype I find no other distinct feature... Thank you very much

Asked by: biohs
Thank you very much for your question. Both mentioned monoclonal antibodies were raised against isolated M13 phage coat proteins and both are recommended for detection of M13 by WB, IF and FCM. Apart from the isoptype there is no differences in the main characteristics. sc-53005 seems to be less suitable for IP applications. Currently, the sc-53004 seems to be the more popular antibody and we already show 1 product citation for this clone.
Answered by: Technical Support Europe
Date published: 2021-02-18

Can you tell me whether this is the same clone as the discontinued GE monoclonal (Cat. # 27-9421-01)? The GE mAb has an unfortunate cross-reaction in one of my assays, so I need to find another one that hopefully will not have this problem.

Asked by: Doctortrout
Thank you for your question. GE's website does not describe a specific clone designation for their now discontinued antibody, so it is unclear which specific clone they were offering. They also do not mention a specific isotype. Therefore it appears that this is not the same antibody that we are providing. M13 Major Coat Protein Antibody (clone designation RL-ph1) is a mouse monoclonal mouse IgG2b: sc-53004. For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to test the antibody before placing your order. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2021-02-18

1- Is the HRP-RL-ph1 conjugate provided as a lyophilized powder? 2- What is the recommended working dilution for ELISA? Thanks

Asked by: ggg27
Thank you for your question. We do not provide any of our antibodies as a lyophilized powder. This antibody, M13 Major Coat Protein (RL-ph1): sc-53004, is available directly conjugated to HRP (catalog number, sc-53004 HRP) and is provided as 200 µg in 1 mL PBS, gelatin-azide buffer. We do not have a specific recommended working dilution for using the HRP conjugated antibody in ELISA. We would recommend a starting dilution of 1:50, and to serially dilute the antibody to determine the optimal ratio of antigen to antibody.
Answered by: Technical Support
Date published: 2021-02-18

Can M13 Major Coat Protein (RL-ph1): sc-53004 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Randy McDonald
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, M13 Major Coat Protein (RL-ph1): sc-53004 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2021-02-18
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Rated 5 out of 5 by from Very good performanceWe were used to using the anti-M13 HRP from GE but it was discontinued, looking for a new brand that would work for ELISA, we received a free unconjugated sample of the anti-M13 from Santa Cruz and we tested it at a dilution of 1: 5000 in 1% BSA -1x PBS. The antibody works well, without background noise. Dilution is appropriate for many ELISA tests and could be optimized.
Date published: 2019-01-28
Rated 4 out of 5 by from goog for ELISAI use this product as detect antibody in phage sandwich ELISA, it is good, no background.
Date published: 2018-09-11
Rated 4 out of 5 by from Suitable replacement for GE product.I was looking to replace the discontinued GE anti-M13 HRP (cat# 27-9421-01). Received a free sample of this unconjugated version and tried it at 1:5000 as with GE, and detected with anti-mouse HRP. Results were comparable, although the GE antibody is supplied 5x more dilute. Will be ordering the HRP conjugated version to compare.
Date published: 2017-08-09
Rated 5 out of 5 by from 灵敏性高，稳定性好我们实验室在做噬菌体库筛选工作中，一直使用HRP标记抗M13抗体，其稳定性好，重复性高，灵敏度也高。
Date published: 2017-07-18
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M13 Major Coat Protein 抗体 (RL-ph1) is rated 4.6 out of 5 by 5.
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