Date published: 2025-9-8

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LYPLA3 抗体 (B-2): sc-376078

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说明书
  • LYPLA3 抗体 B-2 是小鼠单克隆 IgG1 κ,LYPLA3抗体, 在2篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列246-412位于human物种的LYPLA3的C-terminus
  • LYPLA3 抗体 (B-2) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 LYPLA3
  • 抗LYPLA3抗体(B-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取LYPLA3 (B-2): sc-376078免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 LYPLA3 抗体 (B-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与LYPLA3 抗体 (B-2) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

LYPLA3抗体(B-2)是一种IgG1 κ小鼠单克隆LYPLA3抗体(也称为LYPLA3抗体),可通过WB、IP、IF、IHC(P)和ELISA检测人源LYPLA3蛋白。LYPLA3抗体(B-2)既可以是非偶联型抗LYPLA3抗体形式,也可以是多种偶联型抗LYPLA3抗体形式,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。LYPLA3(溶血磷脂酶3)也称为ACS、LLPL或LPLA2(溶酶体磷脂酶A2),在肾脏、胎盘、胰腺、睾丸、脾脏、心脏和骨骼肌中表达最高,遍在表达。LYPLA3定位于溶酶体,不依赖钙,具有酸性pH最佳值。LYPLA3转酰化神经酰胺,增加溶酶体膜对钾离子和质子的通透性。由于其对肺泡巨噬细胞中的磷脂酰乙醇胺和磷脂酰胆碱的高特异性,LYPLA3可能在肺表面活性磷脂降解中发挥关键作用。LYPLA3还可能增强溶酶体的渗透敏感性,导致酶泄漏并诱导细胞凋亡,从而使酶不稳定。LYPLA3被认为可以重塑酰基团并调节某些亲脂性醇的生物和药理活性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

LYPLA3 抗体 (B-2) 参考文献:

  1. 溶酶体磷脂酶 A2, 1-O-甘油酰胺合成酶的克隆和表征。  |  Hiraoka, M., et al. 2002. J Biol Chem. 277: 10090-9. PMID: 11790796
  2. 溶酶体磷脂酶 A2 在肺泡巨噬细胞中选择性表达。  |  Abe, A., et al. 2004. J Biol Chem. 279: 42605-11. PMID: 15294901
  3. 溶血磷脂酶 3 的缺失会增加脂蛋白 E 缺失小鼠的动脉粥样硬化。  |  Taniyama, Y., et al. 2005. Biochem Biophys Res Commun. 330: 104-10. PMID: 15781238
  4. 溶酶体磷脂酶 A2 的结构和功能:催化三元组的鉴定和半胱氨酸残基的作用。  |  Hiraoka, M., et al. 2005. J Lipid Res. 46: 2441-7. PMID: 16106046
  5. 溶酶体磷脂酶 A2 的位置特异性。  |  Abe, A., et al. 2006. J Lipid Res. 47: 2268-79. PMID: 16837646
  6. 溶酶体磷脂酶 A2 和磷脂病  |  Hiraoka, M., et al. 2006. Mol Cell Biol. 26: 6139-48. PMID: 16880524
  7. 磷脂酶 A2 超家族及其组别编号系统。  |  Schaloske, RH. and Dennis, EA. 2006. Biochim Biophys Acta. 1761: 1246-59. PMID: 16973413
  8. 磷脂酶 A2 对溶酶体离子渗透性和渗透敏感性的影响  |  Wang, JW., et al. 2006. Chem Phys Lipids. 144: 117-26. PMID: 16982042
  9. 溶酶体磷脂酶 A2 对亲脂醇的酰化作用  |  Abe, A., et al. 2007. J Lipid Res. 48: 2255-63. PMID: 17626977

订购信息

产品名称产品编号规格价格数量收藏夹

LYPLA3 抗体 (B-2)

sc-376078
200 µg/ml
RMB2377.00

LYPLA3 (B-2): m-IgG Fc BP-HRP 套装

sc-529817
200 µg Ab; 10 µg BP
RMB2662.00

LYPLA3 (B-2): m-IgGκ BP-HRP 套装

sc-522840
200 µg Ab, 40 µg BP
RMB2662.00

LYPLA3 (B-2): m-IgG1 BP-HRP 套装

sc-543705
200 µg Ab; 20 µg BP
RMB2662.00

LYPLA3 抗体 (B-2) AC

sc-376078 AC
500 µg/ml, 25% agarose
RMB3129.00

LYPLA3 抗体 (B-2) HRP

sc-376078 HRP
200 µg/ml
RMB2377.00

LYPLA3 抗体 (B-2) FITC

sc-376078 FITC
200 µg/ml
RMB2482.00

LYPLA3 抗体 (B-2) PE

sc-376078 PE
200 µg/ml
RMB2580.00

LYPLA3 抗体 (B-2) Alexa Fluor® 488

sc-376078 AF488
200 µg/ml
RMB2685.00

LYPLA3 抗体 (B-2) Alexa Fluor® 546

sc-376078 AF546
200 µg/ml
RMB2685.00

LYPLA3 抗体 (B-2) Alexa Fluor® 594

sc-376078 AF594
200 µg/ml
RMB2685.00

LYPLA3 抗体 (B-2) Alexa Fluor® 647

sc-376078 AF647
200 µg/ml
RMB2685.00

LYPLA3 抗体 (B-2) Alexa Fluor® 680

sc-376078 AF680
200 µg/ml
RMB2685.00

LYPLA3 抗体 (B-2) Alexa Fluor® 790

sc-376078 AF790
200 µg/ml
RMB2685.00

Is this antibody expected to react with rat? Can you offer me a sample to test it with rat cells?

Asked by: scason
Thank you for your question! LYPLA3 Antibody (B-2) is only recommended for detection of LYPLA3 of human origin by WB, IP, IF, IHC(P) and ELISA. It is not recommended for use with samples from rat and we do not have any data supporting this use at this point.
Answered by: Tech Support Europe
Date published: 2021-07-29

Can LYPLA3 (B-2): sc-376078 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jerojero
Thank you for your question. Yes, LYPLA3 (B-2): sc-376078 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic stainingExcellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human lung tissue. -SCBT QC
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LYPLA3 抗体 (B-2) is rated 5.0 out of 5 by 2.
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