Date published: 2026-10-19

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Ly-6C 抗体 (ER-MP20): sc-52650

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说明书
  • Ly-6C 抗体 ER-MP20 是大鼠单克隆 IgG2a, 在7篇文献中引用,提供 200 µg/ml
  • 针对mouse来源的巨噬细胞系
  • 推荐用于 mouse 来源的Ly-6C IF, IHC(P) 和 FCM检测
  • 还可偶联藻红蛋白 以及 FITC 用于IF, IHC(P) 和 FCM
  • 目前,我们还没有完成Ly-6C 抗体 (ER-MP20)的首选二抗检测试剂的鉴定。这项工作正在进行中。
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関連項目

编码小鼠同种抗原Ly-6C的基因位于染色体15上,编码一种属于糖基磷脂酰肌醇(GPI)连接蛋白Ly-6家族的131个氨基酸的蛋白质。Ly-6家族成员在整个独特的富含半胱氨酸的蛋白质结构域中具有氨基酸同源性,该结构域结合了O-连接的碳水化合物。小鼠Ly-6分子在多能干细胞到谱系定向前体细胞的造血过程中以及外周淋巴组织中的特定白细胞亚群上具有独特的组织表达模式。Ly-6C主要在小鼠外周CD8 T细胞上表达。Ly-6C参与内皮粘附、CTL对靶细胞的杀伤、诱导CD8 T细胞中TCR介导的IL-2和IFN-γ的产生以及CD8 T细胞的归巢。此外,Ly-6C可能作为LFA-1激活的信号分子。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Ly-6C 抗体 (ER-MP20) 参考文献:

  1. Ly-6超家族成员Ly-6A/E、Ly-6C和Ly-6I识别B淋巴细胞表达的两个潜在配体。  |  Pflugh, DL., et al. 2002. J Immunol. 169: 5130-6. PMID: 12391229
  2. 小鼠 Ly-6C 和 Ly-6G 之间不存在交叉反应。  |  Nagendra, S. and Schlueter, AJ. 2004. Cytometry A. 58: 195-200. PMID: 15057973
  3. 脾脏储备单核细胞的鉴定及其在炎症部位的分布。  |  Swirski, FK., et al. 2009. Science. 325: 612-6. PMID: 19644120
  4. T细胞调节中Ly-6C(hi)髓样细胞的塑性。  |  Zhu, B., et al. 2011. J Immunol. 187: 2418-32. PMID: 21824867
  5. 髓外造血产生浸润动脉粥样硬化病变的Ly-6C(高)单核细胞。  |  Robbins, CS., et al. 2012. Circulation. 125: 364-74. PMID: 22144566
  6. 由 galectin-9 驱动的 CD11b+Ly-6C+ 髓源性抑制细胞(MDSCs)的扩增可减轻 CVB3 诱导的心肌炎。  |  Zhang, Y., et al. 2017. Mol Immunol. 83: 62-71. PMID: 28110209
  7. CD11chi树突状细胞凋亡引起的日本脑炎恶化与调节性Foxp3+和IL-17+CD4+ Th17细胞以及Ly-6Chi和Ly-6Clo单核细胞失衡有关。  |  Choi, JY., et al. 2017. Immune Netw. 17: 192-200. PMID: 28680381
  8. Aire控制表达Ly-6C/Ly-6G的髓质胸腺上皮细胞的横向生成  |  Morimoto, J., et al. 2018. J Immunol. 201: 3244-3257. PMID: 30389776
  9. 处于不同成熟阶段的CD11b+肺树突状细胞可诱导Th17或Th2分化。  |  Izumi, G., et al. 2021. Nat Commun. 12: 5029. PMID: 34413303
  10. Ly-6C在小鼠脑血管内皮细胞中表达,但在小胶质细胞中不表达。  |  Alliot, F., et al. 1998. Neurosci Lett. 251: 37-40. PMID: 9714459

订购信息

产品名称产品编号规格价格数量收藏夹

Ly-6C 抗体 (ER-MP20)

sc-52650
200 µg/ml
CNY2422.00

Ly-6C 抗体 (ER-MP20) FITC

sc-52650 FITC
200 µg/ml
CNY2527.00

Ly-6C 抗体 (ER-MP20) PE

sc-52650 PE
200 µg/ml
CNY2625.00

Can Ly-6C (ER-MP20): sc-52650 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Ly-6C (ER-MP20): sc-52650 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by MartaAgudo from Ly6c immunolabelling of blood vessels in mouseimmunodetection of Ly6c with this antibody labels mouse blood vessels, both veins and arteries, in the central nervous system as well as in peripheral tissue. In this study we used analysed in depth its use as blood vessel marker in the healthy and diseased retina. Very bright and crispy signal. Dilution of primary 1:500
Date published: 2022-01-12
Rated 5 out of 5 by DefinitelyNotMatt from Reliable Ab for IF and FlowGreat for IF of mouse cells, very reliable antibody. Also well-cited for Flow Cytometry.
Date published: 2017-02-01
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Ly-6C 抗体 (ER-MP20) is rated 4.7 out of 5 by 3.
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