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LXR alpha/beta/NR1H3/NR1H2 Antibody (H-7) - Western Blotting - Image 378668 — LXRα/β 抗体 (H-7): sc-377260. THP-1 (A), c4 (B), M1 (C), A549 (D) 和 U-87 MG (E) 全细胞裂解液中 LXRα/β 表达的 Western 印迹分析.

LXRα/β 抗体 (H-7): sc-377260. THP-1 (A), c4 (B), M1 (C), A549 (D) 和 U-87 MG (E) 全细胞裂解液中 LXRα/β 表达的 Western 印迹分析.

LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7): sc-377260

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说明书

LXR alpha/beta/NR1H3/NR1H2 抗体 H-7 是小鼠单克隆 IgG₁ κ，LXR alpha/beta/NR1H3/NR1H2抗体, 在22篇文献中引用，规格为200 µg/ml
特异性抗原位于human物种的LXRβ的C-terminus的氨基酸433-461之间
LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) 推荐用于WB, IP, IF 和 ELISA ，检测 mouse, rat, human 和来源的LXRα and LXRβ; 也和以下物种反应，包括：和 equine, canine, bovine, porcine and avian
抗LXR alpha/beta/NR1H3/NR1H2抗体（H-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
可用于凝胶迁移和ChIP的TransCruz试剂(sc-377260 X, 200 µg/0.1 ml)
m-IgGκ BP-HRP是LXR alpha/beta/NR1H3/NR1H2 Antibody (H-7) 适用于 WB 应用。的首选辅助检测试剂。该试剂现可与LXR alpha/beta/NR1H3/NR1H2 Antibody (H-7) 搭配使用（请参阅下面的订购信息）。有关其它 m-IgGκ BP 结合物，请参阅我们完整的小鼠 IgG 结合蛋白列表。

查看产品引用文献(22)

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LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) 参考文献:

LXRα和LXRβ是内分泌系统的把关者。 | Maqdasy, S., et al. 2016. Mol Aspects Med. 49: 31-46. PMID: 27091047
槲皮素通过调节PCSK9、CD36、PPARγ、LXRα和ABCA1的表达来预防动脉粥样硬化。 | Jia, Q., et al. 2019. Int J Mol Med. 44: 893-902. PMID: 31524223
禁食和禁食模拟治疗可激活SIRT1/LXRα，缓解糖尿病引起的全身和微血管功能障碍。 | Hammer, SS., et al. 2021. Diabetologia. 64: 1674-1689. PMID: 33770194
LXRα激活和Raf抑制可引发肝癌的致死性脂毒性。 | Rudalska, R., et al. 2021. Nat Cancer. 2: 201-217. PMID: 35122079
抑制DHCR24激活LXRα，改善肝脂肪变性及炎症。 | Zhou, E., et al. 2023. EMBO Mol Med. 15: e16845. PMID: 37357756
山柰精油通过激活PPARγ-LXRα-ABCA1/G1信号通路改善动脉粥样硬化。 | Wang, SQ., et al. 2024. Phytomedicine. 123: 155227. PMID: 38128398
IGFBPL1通过增强IGR1R/LXRα/ABCG1通路的激活来抑制巨噬细胞的脂质积累。 | Hou, L., et al. 2023. Aging (Albany NY). 15: 14791-14802. PMID: 38157252
Tiaogan daozhuo formula attenuates atherosclerosis via activating AMPK -PPARγ-LXRα pathway. | Zhang, Y., et al. 2024. J Ethnopharmacol. 324: 117814. PMID: 38286155
亮氨酸通过mTORC1/LXRα信号通路调节巨噬细胞极化，从而缓解细胞因子风暴综合征。 | Yan, H., et al. 2024. Elife. 12: PMID: 38442142
LXRα转录组的重新编程维持巨噬细胞的二次炎症反应。 | Vladimir de la Rosa, J., et al. 2024. Adv Sci (Weinh). 11: e2307201. PMID: 38549193

种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	NR1H2	Entrez Gene ID: 7376	19q13.33	NM_001256647, NM_007121	UniProt ID: P55055	600380
Human	NR1H3	Entrez Gene ID: 10062	11p11.2	XM_005252705, XM_005252706, XM_005252707, XM_005252709, XM_005252710, XM_005252713, XM_005252715, XM_005252716, XM_005252718, XM_006718112, XM_006718113, XM_006718115, XM_006718116, XM_011519805, XM_011519806, XM_011519807, XM_011519808, XM_017017056, XM_017017057, XM_017017058, NM_001130101, NM_001130102, NM_001251934, NM_001251935, NM_005693	UniProt ID: Q13133	602423
Mouse	Nr1h3	Entrez Gene ID: 22259	2 E1	XM_006499166, XM_006499167, XM_006499168, NM_001177730, NM_013839	UniProt ID: Q9Z0Y9	N/A
Mouse	Nr1h2	Entrez Gene ID: 22260	7 B4	XM_006540804, XM_017322126, NM_001285517, NM_001285518, NM_001285519, NM_009473	UniProt ID: Q60644	N/A

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7)	sc-377260	200 µg/ml	CNY2422.00
LXR alpha/beta/NR1H3/NR1H2 (H-7): m-IgGκ BP-HRP 套装	sc-523253	200 µg Ab, 40 µg BP	CNY2715.00
LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) X	sc-377260 X	200 µg/0.1 ml	CNY2422.00
LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) AC	sc-377260 AC	500 µg/ml, 25% agarose	CNY3189.00
LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) HRP	sc-377260 HRP	200 µg/ml	CNY2422.00
LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) FITC	sc-377260 FITC	200 µg/ml	CNY2527.00
LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) PE	sc-377260 PE	200 µg/ml	CNY2625.00
LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) Alexa Fluor^® 488	sc-377260 AF488	200 µg/ml	CNY2738.00
LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) Alexa Fluor^® 546	sc-377260 AF546	200 µg/ml	CNY2738.00
LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) Alexa Fluor^® 594	sc-377260 AF594	200 µg/ml	CNY2738.00
LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) Alexa Fluor^® 647	sc-377260 AF647	200 µg/ml	CNY2738.00
LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) Alexa Fluor^® 680	sc-377260 AF680	200 µg/ml	CNY2738.00
LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) Alexa Fluor^® 790	sc-377260 AF790	200 µg/ml	CNY2738.00
LXR alpha/beta/NR1H3/NR1H2 (H-7) 中和勝肽	sc-377260 P	100 µg/0.5 ml	CNY519.00

What application is the blocking peptide sc-377260 P appropriate for?

Asked by: jenniferc

Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization

Answered by: Technical Support

Date published: 2017-02-24

Rated 4 out of 5 by LaBo from LXRα/β (H-7) western blotting liver and brainLXRα/β expression levels were measured in the brain and the liver of both wt and 3xTg-AD mice brain homogenates. 20 micrograms of total proteins were loaded Bloking overnight in 5% BSA in TTBS Antibody diluted 1:500 in BSA 2% in TTBS Good sensitivity and specificity

Date published: 2024-07-17

Rated 5 out of 5 by Anonymous from 美しいバンドが検出されます。WBで使用しました。シャープなバンドが検出されました。非特異的なバンドは一切ありません。バンドの大きさもデータシートと同じ位置です。

Date published: 2020-12-01

Rated 5 out of 5 by MAO123 from Excellent in WBWestern blotting dilution 1:1000 C2C12 cell lysate specific and very clear band

Date published: 2018-10-05

Rated 2 out of 5 by wenwen20170912 from CHIP failed with sampleI applied a sample to do the CHIP experiment, the sample was the mouse liver, the ultrasound fragmented between 300-800bp, each sample used 3ug antibody, the results shown in the figure

Date published: 2017-09-12

Rated 4 out of 5 by sims from working good!I got this two weeks ago and used 1:1000. working good on my WB.

Date published: 2017-07-21

Rated 5 out of 5 by emilyshenn from great featuresWB 1:500 on mouse liver samples,band is very clear

Date published: 2017-05-08

Rated 5 out of 5 by jerojero from Excellent!This antibody works great in western blot analysis of LXRα/β expression in HeLa and THP-1 whole cell lysates.

Date published: 2017-02-01

Rated 5 out of 5 by wangjie from Worked well for co-IP in mouse macrophagesWe get pretty results in IP assay, our samples are mouse macrophages, The working dilution is 1: 100.

Date published: 2017-01-15

LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) Reviews - page 2

LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) is rated 4.5 out of 5 by 10.

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LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7): sc-377260

LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) 参考文献:

LXR alpha/beta/NR1H3/NR1H2 抗体 (H-7) 规格

替代产品名称: LXRα/β

产品克隆名称: H-7

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG₁ kappa light chain

氨基酸: 433-461

抗原表位靶标: LXRβ

抗原表位种属: human

产品反应性/检测: LXRα and LXRβ

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: equine, canine, bovine, porcine and avian

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 22篇各种科学出版物的引文

優選的 LXR alpha/beta/NR1H3/NR1H2 抗體是 LXR alpha/beta/NR1H3/NR1H2 抗體 (G-10): sc-271064.