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Luciferase Antibody (C-12) - Western Blotting - Image 19561
荧光素酶抗体 (C-12): sc-74548. 重组萤火虫荧光素酶蛋白的 Western 印迹分析.
Luciferase Antibody (C-12) - Western Blotting - Image 19561
荧光素酶抗体 (C-12): sc-74548. 重组萤火虫荧光素酶蛋白的 Western 印迹分析.
Luciferase Antibody (C-12) - Western Blotting - Image 391040
荧光素酶抗体 (C-12) Alexa Fluor® 680: sc-74548 AF680. 直接对萤火虫重组荧光素酶融合蛋白进行近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214.
Luciferase Antibody (C-12) - Western Blotting - Image 395117
荧光素酶抗体 (C-12) HRP: sc-74548 HRP. 萤火虫重组荧光素酶融合蛋白的直接 Western 印迹分析.
荧光素酶抗体 (C-12): sc-74548. 重组萤火虫荧光素酶蛋白的 Western 印迹分析.

Luciferase 抗体 (C-12): sc-74548

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寫評論提問

说明书
  • Luciferase 抗体 C-12 是小鼠单克隆 IgG2a κ，Luciferase抗体, 在29篇文献中引用，规格为200 µg/ml
  • 针对氨基酸251-550的抗原，在Luciferase的C-terminus处映射，Photinus pyralis来源
  • Luciferase 抗体 (C-12) 推荐用于WB, IP, IF 和 ELISA，检测Photinus pyralis 来源的 Luciferase
  • 抗Luciferase抗体（C-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Luciferase (C-12): sc-74548的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是Luciferase Antibody (C-12) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Luciferase Antibody (C-12) 打包提供（请参阅下面的订购信息）。

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描述
蛋白序列
说明书与实验方案
研究信息

関連項目

荧光素酶抗体（C-12）是一种IgG2a κ小鼠单克隆荧光素酶抗体（也称为萤火虫荧光素酶抗体），可通过WB, IP, IF和ELISA检测来自Photinus pyralis的荧光素酶蛋白。荧光素酶抗体（C-12）既有非偶联的抗荧光素酶抗体形式，也有多种偶联形式的抗荧光素酶抗体，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®偶联物。从北美常见萤火虫Photinus pyralis中分离出的荧光素酶是在生物发光生物体中催化光产生的研究最广泛的酶之一。荧光素酶属于依赖ATP的AMP结合酶家族。它产生波长为562 nM的绿光。以下是荧光素酶催化的化学催化反应：Photinus荧光素+ O(2) + ATP = 氧化的Photinus荧光素+ CO(2) + AMP + 二磷酸 + 光。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Luciferase 抗体 (C-12) 参考文献:

  1. 使用同时包含Photinus pyralis和Renilla reniformis荧光素酶基因的新型报告载体同时测量启动子活性和转染效率。  |  Park, JB. 2001. Anal Biochem. 291: 162-6. PMID: 11262171
  2. 作为抗氧化剂和发光体的火蝇荧光素：昆虫生物发光的进化。  |  Dubuisson, M., et al. 2004. Luminescence. 19: 339-44. PMID: 15558801
  3. 利用生物发光报告细胞进行高通量趋化定量分析。  |  Vishwanath, RP., et al. 2005. J Immunol Methods. 302: 78-89. PMID: 15987642
  4. 用于生物发光报告器应用的红色和绿色发光萤火虫荧光素酶突变体。  |  Branchini, BR., et al. 2005. Anal Biochem. 345: 140-8. PMID: 16125663
  5. 生物发光报告物的发射光谱以及与哺乳动物组织的相互作用决定了体内检测的灵敏度。  |  Zhao, H., et al. 2005. J Biomed Opt. 10: 41210. PMID: 16178634
  6. An enhanced chimeric firefly luciferase-inspired enzyme for ATP detection and bioluminescence reporter and imaging applications.  |  Branchini, BR., et al. 2015. Anal Biochem. 484: 148-53. PMID: 26049097
  7. Cloning of the Orange Light-Producing Luciferase from Photinus scintillans-A New Proposal on how Bioluminescence Color is Determined.  |  Branchini, BR., et al. 2017. Photochem Photobiol. 93: 479-485. PMID: 27861940
  8. C-末端结构域对绿色和红色发光甲虫荧光素酶和荧光素酶样酶的生物发光活性和颜色决定的影响。  |  Bevilaqua, VR., et al. 2021. Photochem Photobiol Sci. 20: 113-122. PMID: 33721241
  9. 萤火虫荧光素酶是哺乳动物细胞中过氧物酶体的靶向酶。  |  Keller, GA., et al. 1987. Proc Natl Acad Sci U S A. 84: 3264-8. PMID: 3554235
  10. 萤火虫荧光素酶基因：结构和在哺乳动物细胞中的表达。  |  de Wet, JR., et al. 1987. Mol Cell Biol. 7: 725-37. PMID: 3821727
  11. 全身麻醉剂抑制萤火虫荧光素酶的结构基础。  |  Franks, NP., et al. 1998. Biophys J. 75: 2205-11. PMID: 9788915
  12. 通过体外翻译从成年灯笼获取的 RNA 合成活性萤火虫荧光素酶。  |  Wood, KV., et al. 1984. Biochem Biophys Res Commun. 124: 592-6. PMID: 6208909

订购信息

产品名称产品编号规格价格数量收藏夹

Luciferase 抗体 (C-12)

sc-74548
200 µg/ml
RMB2377.00

Luciferase (C-12): m-IgG Fc BP-HRP 套装

sc-528688
200 µg Ab; 10 µg BP
RMB2662.00

Luciferase (C-12): m-IgGκ BP-HRP 套装

sc-521137
200 µg Ab, 40 µg BP
RMB2662.00

Luciferase (C-12): m-IgG2a BP-HRP 套装

sc-547148
200 µg Ab; 10 µg BP
RMB2662.00

Luciferase 抗体 (C-12) AC

sc-74548 AC
500 µg/ml, 25% agarose
RMB3129.00

Luciferase 抗体 (C-12) HRP

sc-74548 HRP
200 µg/ml
RMB2377.00

Luciferase 抗体 (C-12) FITC

sc-74548 FITC
200 µg/ml
RMB2482.00

Luciferase 抗体 (C-12) PE

sc-74548 PE
200 µg/ml
RMB2580.00

Luciferase 抗体 (C-12) Alexa Fluor® 488

sc-74548 AF488
200 µg/ml
RMB2685.00

Luciferase 抗体 (C-12) Alexa Fluor® 546

sc-74548 AF546
200 µg/ml
RMB2685.00

Luciferase 抗体 (C-12) Alexa Fluor® 594

sc-74548 AF594
200 µg/ml
RMB2685.00

Luciferase 抗体 (C-12) Alexa Fluor® 647

sc-74548 AF647
200 µg/ml
RMB2685.00

Luciferase 抗体 (C-12) Alexa Fluor® 680

sc-74548 AF680
200 µg/ml
RMB2685.00

Luciferase 抗体 (C-12) Alexa Fluor® 790

sc-74548 AF790
200 µg/ml
RMB2685.00

Will luciferase retain its enzyme activity towards luciferin and ATP upon binding to this antibody?

Asked by: LuCMM
Thank you for your question. Luciferase Antibody (C-12): sc-74548 is only recommended for detection of Luciferase of Photinus pyralis origin by WB, IP, IF and ELISA. We do not have data on its performance in assays other than antigen detection.
Answered by: Tech Support Europe
Date published: 2023-11-24

Will storing this firefly luciferase antibody at -20 lead to activity loss?

Asked by: Medha
Thank you for your question. All of our antibodies should be kept at +4 °C for storage to prevent degradation. A single freeze down may impact the antibody's viability and lead to reduced signal strength, but should not render the antibody unusable. We advise testing the antibody and if necessary compensate by increasing the amount of antibody used in your experiments accordingly.
Answered by: Tech Support Europe
Date published: 2023-08-08

Does this antibody react with nanoLuc luciferase from Promega?

Asked by: JC2020
Thank you for your question. This mouse monoclonal Luciferase antibody, sc-74548 was raised against amino acids 251-550 mapping at the C-terminus of Luciferase of Photinus pyralis origin. Unfortunately we do not have any information regarding the nanoLuc Luciferase from Promega, but it does detect standard Luciferace of Photinus pyralis origin.
Answered by: Tech Service
Date published: 2020-01-27

Concerning the IP antibody sc-74548 AC, what kind of bound is it between the agarose beads and the antibody ? A covalent one ?

Asked by: dmr56re
Thank you for your question. Yes, the antibody is covalently bound to the agarose beads via the primary amines.
Answered by: Tech Service
Date published: 2020-01-10

Hi I have a split luciferase expression system where one vector has nLUC (N terminal LUC) and another vector has cLUC (C terminal LUC). I want to know whether, this antibody can detect both N and C terminal LUC? Thanks

Asked by: skarre86
Thank you for your question. This antibody has a C-terminal epitope so it will detect the protein that contains the C-terminal region. Please contact technical service if you need further assistance.
Answered by: Tech Service
Date published: 2019-02-08

Hi, I want to perform western blotting for a modified firefly luciferase p2luc, just wondering if this antibody will work with this modified P2luc

Asked by: vindu
Thank you for your question. Please contact our Technical Service Department and we can assist you by performing sequence analysis of the antigen used to develop the antibody, along with your specific modified p2luc protein sequence.
Answered by: Technical Support
Date published: 2019-01-26

Is there a blocking peptide available for this antibody?

Asked by: AbPolly
Thank you for your question. As this antibody was raised against a recombinant protein, it is not possible to offer a blocking peptide for this product.
Answered by: Technical Support 7
Date published: 2017-01-11

What is the recommended secondary reagent for this antibody?

Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 7
Date published: 2017-01-11
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Rated 5 out of 5 by from FantasticFantastic product! Will order again when needed!!!!
Date published: 2023-01-03
Rated 1 out of 5 by from mAb doesnt work for FacsmAb shows very strong binding to untransfected cells
Date published: 2022-10-27
Rated 5 out of 5 by from Works perfectly for WBworks very well in WB, incubation in PBS-T Milk 5% 1h at 4°C.
Date published: 2021-07-15
Rated 4 out of 5 by from funziona beneQuesto anticorpo produce una banda singola e netta.
Date published: 2019-05-15
Rated 5 out of 5 by from greatfirefly luciferase. Loading 20ug, antibody 1:500 dilution, get band at 62kD. good.
Date published: 2018-01-26
Rated 5 out of 5 by from Excellent resultsthe sample lucferase (C-12) sc-74548 works very well, we did already buy for our lab.
Date published: 2017-11-13
Rated 5 out of 5 by from Excellent antibodyI purchased this antibody two weeks ago. It works very well for western blot analysis.
Date published: 2017-10-27
Rated 5 out of 5 by from Positive resultNice sample antibody.Excelent results by WB on huamn skeletal muscle 1:700 used.
Date published: 2017-04-29
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Luciferase 抗体 (C-12) is rated 4.3 out of 5 by 15.
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