Date published: 2025-9-7
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LRP6 Antibody (C-10) - Western Blotting - Image 356566
LRP6 抗体 (C-10): sc-25317. NRK (A), A-10 (B), L6 (C) 和 RBL-1 (D) 全细胞裂解液中 LRP6 表达的 Western 印迹分析.
LRP6 抗体 (C-10): sc-25317. NRK (A), A-10 (B), L6 (C) 和 RBL-1 (D) 全细胞裂解液中 LRP6 表达的 Western 印迹分析.

LRP6 抗体 (C-10): sc-25317

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寫評論提問

说明书
  • LRP6 抗体 C-10 是小鼠单克隆 IgG2a κ，LRP6抗体, 在52篇文献中引用，规格为200 µg/ml
  • 免疫human物种的LRP6的氨基酸1314-1613
  • LRP6 抗体 (C-10) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 LRP6
  • 抗LRP6抗体（C-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取LRP6 (C-10): sc-25317的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 LRP6 抗体 (C-10) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与LRP6 抗体 (C-10) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 LRP 抗体 包括 LRP1, LRP11, LRP3, LRP5和LRP6

LRP6抗体（C-10）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的LRP6蛋白。抗LRP6抗体（C-10）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。LRP6在Wnt/β-catenin信号传导中起着关键作用，该信号传导途径对于身体轴向模式、中枢神经系统发育、肢体形成和器官发生等许多发育过程至关重要。该信号传导途径对于维持细胞功能和组织稳态至关重要，LRP6失调与包括癌症和代谢紊乱在内的多种疾病有关。人类LRP6基因位于12p13.2染色体上，编码一种蛋白质，其细胞外结构域具有四个表皮生长因子（EGF）重复序列和三个低密度脂蛋白受体（LDLR）重复序列的独特排列，这些序列对于其与Wnt配体的相互作用至关重要。LRP6单克隆抗体（C-10）使研究人员能够深入了解Wnt信号传导的机制及其对健康和疾病的影响。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

LRP6 抗体 (C-10) 参考文献:

  1. 低密度脂蛋白受体相关蛋白与阿尔茨海默病有关吗？  |  Lambert, JC., et al. 1999. Neurogenetics. 2: 109-13. PMID: 10369887
  2. 在缺乏 VLDL 受体和 ApoE 受体 2 的基因敲除小鼠中，Reeler/Disabled 类神经元迁移中断。  |  Trommsdorff, M., et al. 1999. Cell. 97: 689-701. PMID: 10380922
  3. 极低密度脂蛋白受体的功能域：配体结合和酸依赖性配体解离机制的分子分析。  |  Mikhailenko, I., et al. 1999. J Cell Sci. 112 (Pt 19): 3269-81. PMID: 10504332
  4. 内细胞受体蛋白 LRP 还通过 N-甲基-D-天冬氨酸受体介导神经元钙信号。  |  Bacskai, BJ., et al. 2000. Proc Natl Acad Sci U S A. 97: 11551-6. PMID: 11016955
  5. 一种与 LDL 受体相关的蛋白质在小鼠体内介导 Wnt 信号。  |  Pinson, KI., et al. 2000. Nature. 407: 535-8. PMID: 11029008
  6. 低密度脂蛋白受体相关蛋白通路对淀粉样 beta 蛋白清除率和阿尔茨海默氏症易感性的调节。  |  Kang, DE., et al. 2000. J Clin Invest. 106: 1159-66. PMID: 11067868
  7. 下调 LRP6 可促进心肌细胞增殖和心脏再生。  |  Wu, Y., et al. 2021. Cell Res. 31: 450-462. PMID: 32973339
  8. LRP6受体在发育和人类疾病中发挥重要作用。  |  Alrefaei, AF. and Abu-Elmagd, M. 2022. Genes (Basel). 13: PMID: 35052459
  9. 心肌梗死中LRP6介导的连接蛋白43磷酸化。  |  Zhang, XM., et al. 2023. iScience. 26: 106160. PMID: 36879803
  10. 靶向LRP6：癌症治疗的新策略。  |  Xue, W., et al. 2024. Pharmacol Res. 204: 107200. PMID: 38710241
  11. 低密度脂蛋白受体基因家族新成员 LRP6 的分离和特征描述。  |  Brown, SD., et al. 1998. Biochem Biophys Res Commun. 248: 879-88. PMID: 9704021
  12. 低密度脂蛋白受体相关蛋白的三个补体型重复序列确定了 RAP, PAI-1 和乳铁蛋白的共同结合位点。  |  Vash, B., et al. 1998. Blood. 92: 3277-85. PMID: 9787164

订购信息

产品名称产品编号规格价格数量收藏夹

LRP6 抗体 (C-10)

sc-25317
200 µg/ml
RMB2377.00

LRP6 (C-10): m-IgG Fc BP-HRP 套装

sc-528401
200 µg Ab; 10 µg BP
RMB2662.00

LRP6 (C-10): m-IgGκ BP-HRP 套装

sc-520774
200 µg Ab, 40 µg BP
RMB2662.00

LRP6 (C-10): m-IgG2a BP-HRP 套装

sc-547087
200 µg Ab; 10 µg BP
RMB2662.00

LRP6 抗体 (C-10) AC

sc-25317 AC
500 µg/ml, 25% agarose
RMB3129.00

LRP6 抗体 (C-10) HRP

sc-25317 HRP
200 µg/ml
RMB2377.00

LRP6 抗体 (C-10) FITC

sc-25317 FITC
200 µg/ml
RMB2482.00

LRP6 抗体 (C-10) PE

sc-25317 PE
200 µg/ml
RMB2580.00

LRP6 抗体 (C-10) Alexa Fluor® 488

sc-25317 AF488
200 µg/ml
RMB2685.00

LRP6 抗体 (C-10) Alexa Fluor® 546

sc-25317 AF546
200 µg/ml
RMB2685.00

LRP6 抗体 (C-10) Alexa Fluor® 594

sc-25317 AF594
200 µg/ml
RMB2685.00

LRP6 抗体 (C-10) Alexa Fluor® 647

sc-25317 AF647
200 µg/ml
RMB2685.00

LRP6 抗体 (C-10) Alexa Fluor® 680

sc-25317 AF680
200 µg/ml
RMB2685.00

LRP6 抗体 (C-10) Alexa Fluor® 790

sc-25317 AF790
200 µg/ml
RMB2685.00

It says the antibody is targeted against aa 1314-1613 - this spans across the extracellular and intracellular portion of the protein. I need an antibody that recognises the extracellular domain - should this antibody be suitable?

Asked by: DanR
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext. 1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-08-16

I want to stain my mouse tissue samples. How can I do this using a mouse monoclonal antibody?

Asked by: Cweed
We recommend using a directly conjugated antibody. This product, sc-25317, is available directly conjugated to FITC, AF488, and AF647.
Answered by: Technical Support 9
Date published: 2017-01-09
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Rated 3 out of 5 by from buonoutilizzato 1:200 su 3 diverse linee cellulari di neuroblastoma; la banda attesa di 180 kDa è appena visibile anche a lunghe esposizioni; più nitida è una banda a 100 kDa; + altre inferiori lievissime
Date published: 2019-10-22
Rated 5 out of 5 by from Good for western blotThis antibody is good for detecting endogenous LRP6 (such as in MDA-MB-231 cells) by western blot.
Date published: 2019-06-03
Rated 1 out of 5 by from Did not workThis antibody was cited by the Human Protein Atlas for their IHC of human tissue, but I could not get it to work Antigen retrieval: Sodium Citrate pH 6, 5 min high pressure Primary: 1:50, 18 hours DAB development for 20 minutes yielded a very weak signal.
Date published: 2017-09-23
Rated 5 out of 5 by from positive bandI obtained positive band use this antibody ,sample is mouse kidney tissue, it shows the expression.
Date published: 2017-06-15
Rated 5 out of 5 by from IF stainingBeautiful IF staining, with Hep G2 great monoclonal specificity with dilution 1:400.
Date published: 2017-06-03
Rated 5 out of 5 by from strong signalWestern blot(dilution 1:800) results; strong signal and low background.
Date published: 2017-05-27
Rated 5 out of 5 by from good resultWe have used the antibody for WB(1:1000), dissolved in milk, the results were good.
Date published: 2017-05-25
Rated 5 out of 5 by from Robust bandVery strong signal by Western Blot using Jurkat whole cell lysates.
Date published: 2017-05-07
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LRP6 抗体 (C-10) is rated 4.5 out of 5 by 22.
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