Date published: 2025-9-7

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LLGL2 抗体 (A-4): sc-376857

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说明书
  • LLGL2 抗体 A-4 是小鼠单克隆 IgG1 κ,LLGL2抗体, 在8篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列148-205位于human物种的LLGL2的内部区域内
  • LLGL2 抗体 (A-4) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 LLGL2
  • 抗LLGL2抗体(A-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取LLGL2 (A-4): sc-376857免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是LLGL2 Antibody (A-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与LLGL2 Antibody (A-4) 打包提供(请参阅下面的订购信息)。

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関連項目

LLGL2抗体(A-4)是一种IgG1 κ小鼠单克隆LLGL2抗体(也称为LLGL2抗体),可通过WB、IP、IF和ELISA检测到小鼠、大鼠和人类来源的LLGL2蛋白。LLGL2抗体(A-4)既有非偶联形式的抗LLGL2抗体,也有多种偶联形式的抗LLGL2抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。LLGL2(致命巨幼虫同源物2),也被称为HGL或LGL2,是一种皮层细胞骨架蛋白,是更大蛋白质复合物的一部分。该复合物由LGN、PKC i和PAR-6β组成,可能在确保双极纺锤体的正确组织和定向以及正常细胞分裂和上皮细胞极性建立的初始阶段中发挥作用。在黑腹果蝇中,滤泡细胞中的LLGL2对于柄细胞和后部滤泡细胞的分化是必需的。在人类中,LLGL2可能参与细胞增殖控制和肿瘤发生。细胞极化过程中会诱导LLGL2的磷酸化,这可能有助于LLGL2从PKC i和PAR-6β复合物中分离。LLGL2的过度表达被认为会抑制细胞中的紧密连接形成。LLGL2在细胞质中表达,并定位于细胞的核周结构。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

LLGL2 抗体 (A-4) 参考文献:

  1. 果蝇肿瘤抑制因子致死(2)巨幼虫的哺乳动物同源物与Madin-Darby犬肾细胞中的基底外切机制相互作用。  |  Müsch, A., et al. 2002. Mol Biol Cell. 13: 158-68. PMID: 11809830
  2. 有丝分裂过程中 Lgl2 与 LGN 的直接结合及其对正常细胞分裂的要求  |  Yasumi, M., et al. 2005. J Biol Chem. 280: 6761-5. PMID: 15632202
  3. aPKC, Crumbs3 和 Lgl2 在脊椎动物早期发育过程中控制顶基底极性。  |  Chalmers, AD., et al. 2005. Development. 132: 977-86. PMID: 15689379
  4. 在恶性黑色素瘤中,Hugl-1 的表达量大幅减少。  |  Kuphal, S., et al. 2006. Oncogene. 25: 103-10. PMID: 16170365
  5. Scribble 通过不同的分子结构域与两种极性蛋白 Lgl2 和 Vangl2 结合。  |  Kallay, LM., et al. 2006. J Cell Biochem. 99: 647-64. PMID: 16791850
  6. p32 是一种新型哺乳动物 Lgl 结合蛋白,可增强蛋白激酶 Czeta 的活性并调节细胞极性。  |  Bialucha, CU., et al. 2007. J Cell Biol. 178: 575-81. PMID: 17682048
  7. Hugl-2(果蝇致死巨幼虫(lgl)极性基因的人类同源物)启动子的克隆和表征。  |  Zimmermann, T., et al. 2008. Biochem Biophys Res Commun. 366: 1067-73. PMID: 18155665
  8. 转录抑制因子 ZEB1 可促进癌症的转移和细胞极性的丧失。  |  Spaderna, S., et al. 2008. Cancer Res. 68: 537-44. PMID: 18199550
  9. 果蝇卵子发生过程中,卵泡细胞中需要致命(2)巨幼虫来引发最初的AP不对称和卵母细胞极性的形成。  |  Li, Q., et al. 2008. Cell Res. 18: 372-84. PMID: 18268543

订购信息

产品名称产品编号规格价格数量收藏夹

LLGL2 抗体 (A-4)

sc-376857
200 µg/ml
RMB2377.00

LLGL2 (A-4): m-IgG Fc BP-HRP 套装

sc-529946
200 µg Ab; 10 µg BP
RMB2662.00

LLGL2 (A-4): m-IgGκ BP-HRP 套装

sc-523118
200 µg Ab, 40 µg BP
RMB2662.00

LLGL2 (A-4): m-IgG1 BP-HRP 套装

sc-543780
200 µg Ab; 20 µg BP
RMB2662.00

LLGL2 抗体 (A-4) AC

sc-376857 AC
500 µg/ml, 25% agarose
RMB3129.00

LLGL2 抗体 (A-4) HRP

sc-376857 HRP
200 µg/ml
RMB2377.00

LLGL2 抗体 (A-4) FITC

sc-376857 FITC
200 µg/ml
RMB2482.00

LLGL2 抗体 (A-4) PE

sc-376857 PE
200 µg/ml
RMB2580.00

LLGL2 抗体 (A-4) Alexa Fluor® 488

sc-376857 AF488
200 µg/ml
RMB2685.00

LLGL2 抗体 (A-4) Alexa Fluor® 546

sc-376857 AF546
200 µg/ml
RMB2685.00

LLGL2 抗体 (A-4) Alexa Fluor® 594

sc-376857 AF594
200 µg/ml
RMB2685.00

LLGL2 抗体 (A-4) Alexa Fluor® 647

sc-376857 AF647
200 µg/ml
RMB2685.00

LLGL2 抗体 (A-4) Alexa Fluor® 680

sc-376857 AF680
200 µg/ml
RMB2685.00

LLGL2 抗体 (A-4) Alexa Fluor® 790

sc-376857 AF790
200 µg/ml
RMB2685.00

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with LLGL2 (A-4): sc-376857 monoclonal antibody?

Asked by: jenniferc
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 5
Date published: 2017-01-22
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Rated 5 out of 5 by from It's nice antibodyI used this antibody, It is very nice to detect the protein. Thanks
Date published: 2017-11-02
Rated 5 out of 5 by from works well with human samplesa good antibody for western blot, and I got a clean band when incubated the membrane with 1:1000 dilution of primary antibody at 4°C overnight
Date published: 2017-07-25
Rated 5 out of 5 by from Great westernVery nice western blot data; strong signal with low background
Date published: 2017-02-01
Rated 5 out of 5 by from Recommended for WBThis antibody is recommended for detection of LLGL2 of human origin by WB, IP, IF and ELISA.
Date published: 2016-12-23
Rated 4 out of 5 by from Good western blot resultA good western blot result was produced by using this antibody. Sample: recombinant human LLGL2 overexpressed HEK293 cells. But the background is not as clear as the product page shows.
Date published: 2016-12-22
Rated 5 out of 5 by from WB publishable data with human KM12C and KM12SMWB publishable data with human KM12C and KM12SM - SCBT Publication Review
Date published: 2013-11-23
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LLGL2 抗体 (A-4) is rated 4.8 out of 5 by 6.
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