Date published: 2025-9-7
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LIS1 Antibody (G-3) - Western Blotting - Image 381790
LIS1 抗体 (G-3): sc-17817. HeLa (A), C6 (B) 和 BC3H1 (C) 全细胞裂解液中 LIS1 表达的 Western 印迹分析.
LIS1 抗体 (G-3): sc-17817. HeLa (A), C6 (B) 和 BC3H1 (C) 全细胞裂解液中 LIS1 表达的 Western 印迹分析.

LIS1 抗体 (G-3): sc-17817

5.0(4)
寫評論提問

说明书
  • LIS1抗体(G-3)是小鼠单克隆IgG2b κ, 在1篇文献中引用，规格为200 µg/ml
  • 免疫human物种的lissencephaly-1的氨基酸1-300
  • 推荐用于 mouse, rat 和 human 来源的LIS1 WB, IP, IF 和 IHC(P)检测
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 LIS1 抗体 (G-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与LIS1 抗体 (G-3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

无脑畸形（光滑脑）是一种脑发育异常，其特征是不完全的神经元迁移和光滑的脑表面，导致严重的智力迟钝。遗传分析鉴定出两种在某些无脑畸形病例中发生突变的蛋白质，称为无脑畸形-1蛋白（LIS1）和doublecortin。LIS1与异三聚体G蛋白的b亚基具有序列同源性。doublecortin包含一个共识的Abl磷酸化位点，它与预测的激酶蛋白具有一些序列同源性。这两种蛋白质在发育中的大脑中高度表达，表明它们可能参与对脑发育至关重要的信号转导通路。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

LIS1 抗体 (G-3) 参考文献:

  1. LIS1 与神经上皮细胞的纺锤体定向  |  Schwamborn, JC. and Knoblich, JA. 2008. Cell Stem Cell. 2: 193-4. PMID: 18371441
  2. LIS1 在正常发育和疾病中的功能  |  Reiner, O. and Sapir, T. 2013. Curr Opin Neurobiol. 23: 951-6. PMID: 23973156
  3. Lis1突变会阻止小鼠基底放射状胶质样细胞的产生。  |  Penisson, M., et al. 2022. Hum Mol Genet. 31: 942-957. PMID: 34635911
  4. Lis1调控细胞质动力蛋白-1的结构基础  |  Gillies, JP., et al. 2022. Elife. 11: PMID: 34994688
  5. LIS1和NDEL1调控成熟神经元线粒体的轴突运输  |  Pandey, JP., et al. 2022. Front Mol Neurosci. 15: 841047. PMID: 35465088
  6. Lis1-dynein驱动电晕压实并限制微管在动点上的错误附着。  |  Mitevska, O., et al. 2023. J Cell Sci. 136: PMID: 36274587
  7. LIS1 RNA结合以AGO2依赖和独立的方式协调胚胎干细胞的机械敏感特性。  |  Kshirsagar, A., et al. 2023. Nat Commun. 14: 3293. PMID: 37280197
  8. 细胞质驱动蛋白-2概览。  |  Karasmanis, EP., et al. 2023. Nat Struct Mol Biol. 30: 1357-1364. PMID: 37620585
  9. Lis1 可减缓力诱导的细胞质动力蛋白与微管的分离。  |  Kusakci, E., et al. 2024. Nat Chem Biol. 20: 521-529. PMID: 37919547
  10. LIS1 组装动力蛋白-动力蛋白复合体的分子机制。  |  Singh, K., et al. 2024. Science. 383: eadk8544. PMID: 38547289

订购信息

产品名称产品编号规格价格数量收藏夹

LIS1 抗体 (G-3)

sc-17817
200 µg/ml
RMB2377.00

LIS1 (G-3): m-IgGκ BP-HRP 套装

sc-533744
200 µg Ab; 40 µg BP
RMB2662.00

LIS1 (G-3): m-IgG2b BP-HRP 套装

sc-549237
200 µg Ab; 10 µg BP
RMB2662.00

Can LIS1 (G-3): sc-17817 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: TinTin
Thank you for your question. Yes, LIS1 (G-3): sc-17817 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Excellent IF detection at kinetochores & NECells fixed in PTEMF for 10min, blocked in PBS+3% BSA and incubated with anti-lis1 at 1/400 for 1hr at RT.
Date published: 2021-07-03
Rated 5 out of 5 by from Excellent for IP, not so great for WBThis is about the best IP I have seen, completely depletes lysate of LIS1 protein. Definitely go for AC if plan on using in IP. Used with multiple lysis/IP including CelLytic M, M-PER and RIPA. However, did not have great success with WB, specific signal is weak relative to unspecific bands and background.
Date published: 2018-04-13
Rated 5 out of 5 by from WB publishable data with mice testis tissueWB publishable data with mice testis tissue - SCBT Publication Review
Date published: 2015-03-13
Rated 5 out of 5 by from Produced decent immunoperoxidase nuclearProduced decent immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human testis tissue. -SCBT QC
Date published: 2013-12-01
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LIS1 抗体 (G-3) is rated 5.0 out of 5 by 4.
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