Date published: 2025-9-7
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LEKTI Antibody (E-9) - Western Blotting - Image 359517
LEKTI 抗体 (E-9): sc-137109. CCD-1064Sk (A), SK-N-SH (B), RAW 264. 7 (C), WEHI-231 (D), RPE-J (E) 和 RBL-1 (F) 全细胞裂解液中 LEKTI 表达的 Western 印迹分析.
LEKTI 抗体 (E-9): sc-137109. CCD-1064Sk (A), SK-N-SH (B), RAW 264. 7 (C), WEHI-231 (D), RPE-J (E) 和 RBL-1 (F) 全细胞裂解液中 LEKTI 表达的 Western 印迹分析.

LEKTI 抗体 (E-9): sc-137109

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说明书
  • LEKTI 抗体 E-9 是小鼠单克隆 IgG2a κ，LEKTI抗体, 在1篇文献中引用，规格为200 µg/ml
  • 针对氨基酸261-560的图谱，在LEKTI的内部区域，human起源
  • LEKTI 抗体 (E-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 LEKTI precursor and mature form and HF7665 active peptide
  • 抗LEKTI抗体（E-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取LEKTI (E-9): sc-137109的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 LEKTI 抗体 (E-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与LEKTI 抗体 (E-9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

LEKTI抗体（E-9）是一种IgG2a κ小鼠单克隆LEKTI抗体（也称为LEKTI抗体），可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类来源的LEKTI蛋白。LEKTI抗体（E-9）既有非偶联抗LEKTI抗体形式，也有多种偶联形式的抗LEKTI抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。淋巴上皮Kazal型抑制剂（LEKTI）是一种丝氨酸蛋白酶抑制剂，可保护粘膜上皮免受微生物攻击和炎症。LEKTI是上皮分化的标志，在表皮的颗粒层和最上层的棘层以及分层的上皮的分化层中强烈表达。编码LEKTI的SPINK5基因的缺陷是内瑟顿综合征的原因，这是一种严重的常染色体隐性遗传病，其特征为特应性皮炎、花粉热和其他病症。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

LEKTI 抗体 (E-9) 参考文献:

  1. LEKTI，一种新型 15 域人类丝氨酸蛋白酶抑制剂。  |  Mägert, HJ., et al. 1999. J Biol Chem. 274: 21499-502. PMID: 10419450
  2. LEKTI：一种具有病理生理意义的多域丝氨酸蛋白酶抑制剂。  |  Mägert, HJ., et al. 2002. Int J Biochem Cell Biol. 34: 573-6. PMID: 11943586
  3. 人类 15 域丝氨酸蛋白酶抑制剂 LEKTI 的生化特征, 分子生物学和临床意义。  |  Walden, M., et al. 2002. Biol Chem. 383: 1139-41. PMID: 12437098
  4. LEKTI 对丝氨酸蛋白酶 plasmin, trypsin, subtilisin A, cathepsin G 和 elastase 的抑制作用：动力学分析。  |  Mitsudo, K., et al. 2003. Biochemistry. 42: 3874-81. PMID: 12667078
  5. 具有不同折叠的同源蛋白：多种卡扎尔型阻抑剂 LEKTI 的第 1 和第 6 结构域的三维结构。  |  Lauber, T., et al. 2003. J Mol Biol. 328: 205-19. PMID: 12684009
  6. LEKTI 在人类原代角质细胞中的蛋白水解过程, 组织分布以及在 Netherton 综合征中的表达缺陷。  |  Bitoun, E., et al. 2003. Hum Mol Genet. 12: 2417-30. PMID: 12915442
  7. SPINK5和奈瑟顿综合征：新突变、缺失LEKTI的证明以及转谷氨酰胺酶的差异表达。  |  Raghunath, M., et al. 2004. J Invest Dermatol. 123: 474-83. PMID: 15304086
  8. 丝氨酸蛋白酶活性和残留的LEKTI表达决定了奈瑟顿综合征的表型。  |  Hachem, JP., et al. 2006. J Invest Dermatol. 126: 1609-21. PMID: 16601670
  9. SPINK5的杂合性缺失突变与G1258A多态性结合会导致LEKTI功能受损和皮肤屏障蛋白异常表达。  |  Di, WL., et al. 2009. Br J Dermatol. 161: 404-12. PMID: 19438860
  10. 角质细胞特异性间胰蛋白酶通过allikrein激活和LEKTI降解促进脱屑过程。  |  Miyai, M., et al. 2014. J Invest Dermatol. 134: 1665-1674. PMID: 24390132
  11. 异位性皮炎角质层过度角化与KLK7分泌不全和LEKTI表达上调有关。  |  Igawa, S., et al. 2017. J Invest Dermatol. 137: 449-456. PMID: 27769847
  12. MiR-375-3p通过靶向YAP1/LEKTI通路减轻HaCaT细胞的炎症程度。  |  Cheng, S., et al. 2020. Biosci Biotechnol Biochem. 84: 2005-2013. PMID: 32564679

订购信息

产品名称产品编号规格价格数量收藏夹

LEKTI 抗体 (E-9)

sc-137109
200 µg/ml
RMB2377.00

LEKTI (E-9): m-IgG Fc BP-HRP 套装

sc-528862
200 µg Ab; 10 µg BP
RMB2662.00

LEKTI (E-9): m-IgGκ BP-HRP 套装

sc-521428
200 µg Ab, 40 µg BP
RMB2662.00

LEKTI (E-9): m-IgG2a BP-HRP 套装

sc-547194
200 µg Ab; 10 µg BP
RMB2662.00

LEKTI 抗体 (E-9) AC

sc-137109 AC
500 µg/ml, 25% agarose
RMB3129.00

LEKTI 抗体 (E-9) HRP

sc-137109 HRP
200 µg/ml
RMB2377.00

LEKTI 抗体 (E-9) FITC

sc-137109 FITC
200 µg/ml
RMB2482.00

LEKTI 抗体 (E-9) PE

sc-137109 PE
200 µg/ml
RMB2580.00

LEKTI 抗体 (E-9) Alexa Fluor® 488

sc-137109 AF488
200 µg/ml
RMB2685.00

LEKTI 抗体 (E-9) Alexa Fluor® 546

sc-137109 AF546
200 µg/ml
RMB2685.00

LEKTI 抗体 (E-9) Alexa Fluor® 594

sc-137109 AF594
200 µg/ml
RMB2685.00

LEKTI 抗体 (E-9) Alexa Fluor® 647

sc-137109 AF647
200 µg/ml
RMB2685.00

LEKTI 抗体 (E-9) Alexa Fluor® 680

sc-137109 AF680
200 µg/ml
RMB2685.00

LEKTI 抗体 (E-9) Alexa Fluor® 790

sc-137109 AF790
200 µg/ml
RMB2685.00

1-Would be thankful to let me know which coating solution would be the best to use for sitting the E-9 antibody on the plate as the capture AB in the sandwich Elisa. 2- What is the recommended capture and detection concentration in Elisa for this AB?

Asked by: MaryAzi
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-03-02

What is the recommend dilution of the Ab for WB? Is this Ab was used for WB application in published works?

Asked by: OSar
Thank you for your question. For this antibody, the recommended starting dilution is 1:100, with a dilution range between 1:100-1:1000. as of yet, there are no publications citing use of this antibody in western blots. Anti-LEKTI Antibody (E-9) is recommended for detection of LEKTI precursor and mature form and HF7665 active peptide of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
Answered by: Tech Support Europe
Date published: 2022-01-11

Does the unmasking have to be performed with citrate or EDTA for IHC on paraffin-embedded tissues ?

Asked by: ManB
Thank you for your question. You can find our recommended protocol by following this link: https://www.scbt.com/de/resources/protocols/immunoperoxidase-staining. We recommend Heat treatment: Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0 (sc-294091); or with 50 mM glycine-HCl buffer (sc-29096), pH 3.5, with 0.01% (w/v) EDTA (sc-29092). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides.
Answered by: Tech Support Europe
Date published: 2019-11-22

I would like to know if this antibody has been tested for IHC in FFPE sections of mouse epidermis. 

Asked by: ElaineZ
Thank you for your question. This antibody has been tested for IHC in FFPE tissue sections, or for IHC(P) in human tonsil. We have not tested this in mouse epidermis.
Answered by: Technical Support
Date published: 2019-04-20

What is the recommended fixation method for immunofluorescence staining with LEKTI (E-9): sc-137109 monoclonal antibody?

Asked by: Randy McDonald
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent StainingGreat signal when staining cryosectioned, 4% paraformaldehyde fixed human epidermal tissue
Date published: 2017-11-09
Rated 5 out of 5 by from Great immunofluorescence cytoplasmic stainingGreat immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-05
Rated 5 out of 5 by from Great Western Blot data of LEKTI expressionGreat Western Blot data of LEKTI expression in HeLa, Hs 732. -SCBT QC
Date published: 2013-12-07
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LEKTI 抗体 (E-9) is rated 5.0 out of 5 by 3.
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