Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
LDH-B Antibody (431.1) - Western Blotting - Image 368558
LDH-B 抗体 (431. 1): sc-100775. HeLa (A), IMR-32 (B) 和 A-10 (C) 全细胞裂解液以及小鼠心脏 (D), 大鼠心脏 (E) 和人类心脏 (F) 组织提取物中 LDH-B 表达的 Western 印迹分析.
LDH-B 抗体 (431. 1): sc-100775. HeLa (A), IMR-32 (B) 和 A-10 (C) 全细胞裂解液以及小鼠心脏 (D), 大鼠心脏 (E) 和人类心脏 (F) 组织提取物中 LDH-B 表达的 Western 印迹分析.

LDH-B 抗体 (431.1): sc-100775

4.9(16)
寫評論提問

说明书
  • LDH-B 抗体 431.1 是小鼠单克隆 IgG1 κ，LDH-B抗体, 在25篇文献中引用，规格为100 µg/ml
  • 免疫human物种的重组LDH-B
  • 推荐用于 mouse, rat 和 human 来源的LDH-B WB, IP, IF, IHC(P) 和 ELISA检测
  • 看 LDH (H-10): sc-133123，可查看有标记的 LDH-B 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取LDH-B (431.1): sc-100775的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 LDH-B 抗体 (431.1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与LDH-B 抗体 (431.1) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 LDH 抗体包括 LDH-A, LDH-B, LDH-C 和 LDH-D

LDH-B抗体（431.1）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人源LDH-B蛋白。抗LDH-B抗体（431.1）以非结合形式提供。乳酸脱氢酶B（LDH-B）蛋白在细胞代谢中起着至关重要的作用，特别是在L-乳酸转化为丙酮酸的过程中，后者对于厌氧条件下能量的产生至关重要。这种酶活性对于依赖厌氧糖酵解的组织至关重要，例如心肌，其中LDH-B的表达占主导地位。LDH-B的正常运作不仅对能量代谢很重要，而且对维持体内乳酸和丙酮酸的平衡也很重要，因为乳酸和丙酮酸会影响各种生理过程和代谢途径。LDH-B水平的变化与多种疾病有关，包括心脏病和某些癌症，因此LDH-B是临床诊断的重要生物标志物。乳酸脱氢酶家族由三个成员组成--LDH-A、LDH-B和LDH-C，它们在组织中的分布模式各不相同，其中LDH-B在心脏中尤为重要。编码人类LDH-A和LDH-C的基因位于11号染色体上，而人类LDH-B基因则位于12号染色体上，这突出了其表达和功能的遗传基础。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

LDH-B 抗体 (431.1) 参考文献:

  1. [肿瘤标志物在生殖细胞瘤治疗中的作用]。  |  Niwakawa, M. and Tobisu, K. 2001. Gan To Kagaku Ryoho. 28: 1159-65. PMID: 11525037
  2. 人类乳酸脱氢酶（LDH）B（H）变体基因突变的分子特征。  |  Sudo, K., et al. 1992. Hum Genet. 89: 158-62. PMID: 1587525
  3. 大肠杆菌表达的人类睾丸特异性乳酸脱氢酶的特性。  |  LeVan, KM. and Goldberg, E. 1991. Biochem J. 273 (Pt 3): 587-92. PMID: 1996957
  4. 决定人类精子特异性乳酸脱氢酶 LDHC 的基因座与 LDHA 同源。  |  Edwards, YH., et al. 1987. Dev Genet. 8: 219-32. PMID: 2844458
  5. 人类睾丸乳酸脱氢酶-C 基因在与 LDH-A（肌肉）和 LDH-B（心脏）基因同源的位置上被六个内含子打断。  |  Takano, T. and Li, SS. 1989. Biochem Biophys Res Commun. 159: 579-83. PMID: 2930531
  6. 乳酸脱氢酶B调节肿瘤微环境中巨噬细胞的新陈代谢  |  Frank, AC., et al. 2021. Theranostics. 11: 7570-7588. PMID: 34158867
  7. 单细胞RNA测序和孟德尔随机化的综合分析发现，乳酸脱氢酶B是缺血性中风中褪黑激素的靶标。  |  Shi, F., et al. 2024. CNS Neurosci Ther. 30: e14741. PMID: 38702940
  8. 乳酸脱氢酶B在KRAS驱动的肺癌中以非典型方式促进铁死亡防御。  |  Zhao, L., et al. 2024. Cell Death Differ.. PMID: 39643712
  9. T抑制因子对抗LDH-B反应的操纵。  |  Ikezawa, Z., et al. 1984. J Immunol. 132: 1605-7. PMID: 6607942
  10. 乳酸脱氢酶 M-亚基缺乏症：临床特征, 代谢背景和遗传异质性。  |  Kanno, T. and Maekawa, M. 1995. Muscle Nerve Suppl. 3: S54-60. PMID: 7603529
  11. 乳酸脱氢酶的分离方法和临床意义。  |  Kopperschläger, G. and Kirchberger, J. 1996. J Chromatogr B Biomed Appl. 684: 25-49. PMID: 8906464
  12. 超嗜热细菌 thermotoga maritima 的乳酸脱氢酶：2.1 A 分辨率的晶体结构揭示了蛋白质内在稳定的策略。  |  Auerbach, G., et al. 1998. Structure. 6: 769-81. PMID: 9655830

订购信息

产品名称产品编号规格价格数量收藏夹

LDH-B 抗体 (431.1)

sc-100775
100 µg/ml
RMB2504.00

LDH-B (431.1): m-IgG Fc BP-HRP 套装

sc-537288
100 µg Ab; 10 µg BP
RMB2662.00

LDH-B (431.1): m-IgGκ BP-HRP 套装

sc-534520
100 µg Ab; 40 µg BP
RMB2662.00

LDH-B (431.1): m-IgG1 BP-HRP 套装

sc-545125
100 µg Ab; 20 µg BP
RMB2662.00

Will this antibody (LDH-B, 431.1, sc-100775) detect LDH-B in bovine heart extracts using WB analysis Thanks.?

Asked by: DrM1
Thank you for your question. This antibody hasn’t been tested in bovine samples. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined, so we can not perform sequence analysis at this time to predict potential cross reactivity with that species.
Answered by: Technical Support
Date published: 2019-01-26

Is LDH-B (431.1): sc-100775 available directly conjugated to HRP?

Asked by: jenniferc
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at [email protected] Alternatively, consider using LDH (H-10): sc-133123, which is available conjugated to agarose, HRP, FITC, PE, and more. (https://www.scbt.com/scbt/product/ldh-antibody-h-10)
Answered by: Technical Support 5
Date published: 2017-01-19
  • y_2025, m_9, d_6, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_zh_CN, sid_100775, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 102ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from LDHB western blotWe highly recommended for western blot. With 1/800 diluition the staining teh signal is great.
Date published: 2020-09-14
Rated 5 out of 5 by from Perfect antibodyVery happy with this antibody. Highly recommended for western blotting.
Date published: 2019-12-04
Rated 5 out of 5 by from Clean and specific LDH-B antibodyWe were able to generate a high-quality Western blot using the LDH-B antibody on our mouse liver tissue samples. The data has been published (Trends Cell Mol Biol 13:47-56,2018).
Date published: 2019-03-13
Rated 5 out of 5 by from Very specific and and the only mAb for mouseThis antibody is the only monoclonal on the market that can be used to probe mouse samples (only polyclonals available from other vendors), at least according to this reviewer's research. I found it very specific and that it can be diluted up to 1:1000, which is impressive given that this antibody comes pretty diluted already (100µg in 1 mL). I used SantaCruz mouse binding protein HRP (sc-516102) and 5% milk for blocking; incubation - 4oC overnight.
Date published: 2018-11-20
Rated 5 out of 5 by from Great antibodyExcellent antibody. I use 1:500 dilution. It is totally enough!
Date published: 2018-04-17
Rated 5 out of 5 by from Great antibody!Great antibody! Very strong signal, diluted 1:2000 for western blotting
Date published: 2018-02-27
Rated 5 out of 5 by from works very well with mouse epithelial cellsworks great in western blot analysis of LDH-B expression
Date published: 2018-02-01
Rated 5 out of 5 by from immunofluorescenceLDH-B worked well with a concentration of 1:100 on 4% formalin fixed tissue sections
Date published: 2018-01-24
  • y_2025, m_9, d_6, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_16
  • loc_zh_CN, sid_100775, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 19ms
  • REVIEWS, PRODUCT
LDH-B 抗体 (431.1) is rated 4.9 out of 5 by 16.
  • y_2025, m_9, d_6, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_16
  • loc_zh_CN, sid_100775, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 168ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。