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LCAT 抗体 (D-2): sc-376682

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说明书
  • LCAT抗体(D-2)是小鼠单克隆IgG1 κ, 在1篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列283-423位于human物种的LCAT的C-terminus附近
  • LCAT 抗体 (D-2) 推荐用于WB, IP, IF 和 ELISA,检测human 来源的 precursor and mature LCAT
  • 抗LCAT抗体(D-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取LCAT (D-2): sc-376682免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是LCAT Antibody (D-2) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与LCAT Antibody (D-2) 打包提供(请参阅下面的订购信息)。

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関連項目

LCAT抗体(D-2)是一种IgG1 κ小鼠单克隆LCAT抗体,能够通过WB, IP, IF 和 ELISA检测人类来源的LCAT前体和成熟体。LCAT抗体(D-2)既有非偶联形式的抗LCAT抗体,也有多种偶联形式的抗LCAT抗体,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®偶联物。脂肪酶基因家族是在生物体中发现的最强大的遗传超家族之一,包括酯酶和硫酯酶。AB水解酶亚家族的成员包括肝脂肪酶(HL)、内皮脂肪酶(EL)、脂蛋白脂肪酶(LPL)、胰脂肪酶(PL)、胃脂肪酶(GL)和卵磷脂-胆固醇酰基转移酶(LCAT)。这些家族成员在脂质代谢中起着至关重要的作用。LCAT能够使胆固醇酯化,这对于胆固醇的运输是必需的。LCAT的缺乏与鱼眼病有关,这是一种罕见的高密度脂蛋白(HDL)代谢遗传性疾病。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

LCAT 抗体 (D-2) 参考文献:

  1. 人类卵磷脂:胆固醇酰基转移酶基因第 4 外显子中一个新的同卵双核苷酸缺失导致经典的 LCAT 缺乏症,该缺失引起了帧移位和残基 144 处的终止密码子。  |  Teh, EM., et al. 1999. Atherosclerosis. 146: 141-51. PMID: 10487497
  2. 卵磷脂胆固醇酰基转移酶基因 T13M 突变导致鱼眼病。  |  Miida, T., et al. 2004. Clin Chim Acta. 343: 201-8. PMID: 15115696
  3. 胆固醇逆向运输的分子机制:前β迁移高密度脂蛋白与血浆卵磷脂/胆固醇酰基转移酶的反应。  |  Nakamura, Y., et al. 2004. Biochemistry. 43: 14811-20. PMID: 15544352
  4. 胆固醇酯转移蛋白和卵磷脂:胆固醇酰基转移酶对高密度脂蛋白大小分布的贡献。  |  Huesca-Gómez, C., et al. 2004. Endocr Res. 30: 403-15. PMID: 15554357
  5. 家族性 LCAT 缺乏症和鱼眼病。  |  McIntyre, N. 1988. J Inherit Metab Dis. 11 Suppl 1: 45-56. PMID: 3141686

订购信息

产品名称产品编号规格价格数量收藏夹

LCAT 抗体 (D-2)

sc-376682
200 µg/ml
RMB2377.00

LCAT (D-2): m-IgG Fc BP-HRP 套装

sc-529920
200 µg Ab; 10 µg BP
RMB2662.00

LCAT (D-2): m-IgGκ BP-HRP 套装

sc-523043
200 µg Ab, 40 µg BP
RMB2662.00

LCAT (D-2): m-IgG1 BP-HRP 套装

sc-543765
200 µg Ab; 20 µg BP
RMB2662.00

LCAT 抗体 (D-2) AC

sc-376682 AC
500 µg/ml, 25% agarose
RMB3129.00

LCAT 抗体 (D-2) HRP

sc-376682 HRP
200 µg/ml
RMB2377.00

LCAT 抗体 (D-2) FITC

sc-376682 FITC
200 µg/ml
RMB2482.00

LCAT 抗体 (D-2) PE

sc-376682 PE
200 µg/ml
RMB2580.00

LCAT 抗体 (D-2) Alexa Fluor® 488

sc-376682 AF488
200 µg/ml
RMB2685.00

LCAT 抗体 (D-2) Alexa Fluor® 546

sc-376682 AF546
200 µg/ml
RMB2685.00

LCAT 抗体 (D-2) Alexa Fluor® 594

sc-376682 AF594
200 µg/ml
RMB2685.00

LCAT 抗体 (D-2) Alexa Fluor® 647

sc-376682 AF647
200 µg/ml
RMB2685.00

LCAT 抗体 (D-2) Alexa Fluor® 680

sc-376682 AF680
200 µg/ml
RMB2685.00

LCAT 抗体 (D-2) Alexa Fluor® 790

sc-376682 AF790
200 µg/ml
RMB2685.00

Hi I am wondering whether this antibody works for immunostaining? Have ever tried this Abs for immunostaining, either fixed cells or paraffin embedded sample? I would like to stain the cells after the fixation with 4%PFA or methanol. Shin Ogawa 

Asked by: Shin Ogawa
This primary antibody is recommended and warrantied for IF (immunofluorescent staining of fresh or frozen tissues or cells fixed in methanol or acetone), however we are still gathering data to show on our website. Check back for updates!
Answered by: Technical Support
Date published: 2019-06-16

For Western Blot, is it recommended to use denatured or non-denatured conditions with LCAT (D-2): sc-376682 monoclonal antibody?

Asked by: cjMara
Thank you for your question. We recommend this antibody for use in denatured Western Blot conditions. It has not been validated for use in non-denatured conditions. Please contact our Technical Service Department for further details or inquiries.
Answered by: Technical Support
Date published: 2017-02-27
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Rated 1 out of 5 by from No SignalUsed at a 1:500 dilution on 35ug of HeLa lysate and did not get any bands after 30min of exposure
Date published: 2016-12-06
Rated 5 out of 5 by from Produced positive Western blot data of LCATProduced positive Western blot data of LCAT expression in non-transfected and human LCAT transfected MLN whole cell lysates. -SCBT QC
Date published: 2014-07-22
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LCAT 抗体 (D-2) is rated 3.0 out of 5 by 2.
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