Date published: 2025-9-8
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LAT1 Antibody (D-10) - Western Blotting - Image 149459
LAT1 抗体 (D-10): sc-374232. Western 印迹分析 MCF7 全细胞裂解液中 LAT1 的表达.
LAT1 抗体 (D-10): sc-374232. Western 印迹分析 MCF7 全细胞裂解液中 LAT1 的表达.

LAT1 抗体 (D-10): sc-374232

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说明书
  • LAT1 抗体 D-10 是小鼠单克隆 IgG1 κ，LAT1抗体, 在33篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列365-507位于human物种的LAT1的C-terminus
  • LAT1 抗体 (D-10) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 LAT1
  • 抗LAT1抗体（D-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取LAT1 (D-10): sc-374232的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是LAT1 Antibody (D-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与LAT1 Antibody (D-10) 打包提供（请参阅下面的订购信息）。

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描述
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说明书与实验方案
研究信息

関連項目

LAT1抗体（D-10）是一种IgG1κ小鼠单克隆LAT1抗体（也称为溶质载体家族7成员5抗体、溶质载体家族7（氨基酸转运蛋白轻链L系统）成员5抗体、大型中性氨基酸转运蛋白小亚基1抗体、L型氨基酸转运蛋白1抗体、CD98轻链抗体、CD98抗体、溶质载体家族7（阳离子氨基酸转运蛋白Y+系统）成员5抗体、钠离子不依赖中性氨基酸转运蛋白LAT1抗体、Y+系统阳离子氨基酸转运蛋白抗体或CD98LC抗体），可通过WB、IP、IF和ELISA方法检测来自小鼠、大鼠和人类的LAT1蛋白。LAT1抗体（D-10）以非偶联型抗LAT1抗体形式提供，同时也有多种偶联形式的抗LAT1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。L型氨基酸转运蛋白1（LAT1）是一种多通道膜蛋白，负责钠离子不依赖的大型中性氨基酸的高亲和力转运。LAT1与氨基酸转运蛋白CD98以二硫键连接形成异二聚体而发挥作用。LAT1主要在成人肺和肝脏中表达，但也表达于脑、胸腺、视网膜、睾丸、胎盘、骨髓和胎儿肝脏中。在视网膜中，LAT1定位于血视网膜屏障（BRB），介导L-亮氨酸从血液到视网膜的转运。苯丙酮尿症对大脑的毁灭性影响是由于血液中高浓度的苯丙氨酸导致血脑屏障上LAT1水平升高所致。LAT1接受与氨基酸相关的抗癌药物美法仑，并在食管鳞状细胞癌的细胞增殖、分化和侵袭中发挥重要作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

LAT1 抗体 (D-10) 参考文献:

  1. 人类 L 型氨基酸转运体 1（LAT1）：肿瘤细胞系中的功能和表达特征。  |  Yanagida, O., et al. 2001. Biochim Biophys Acta. 1514: 291-302. PMID: 11557028
  2. 大鼠肝转移模型中 L 型氨基酸转运体 1 的表达：与肿瘤大小呈正相关。  |  Tamai, S., et al. 2001. Cancer Detect Prev. 25: 439-45. PMID: 11718450
  3. L 型氨基酸转运体 1（LAT1）和 4F2 重链（4F2hc）在大鼠模型肝肿瘤病变中的表达。  |  Ohkame, H., et al. 2001. J Surg Oncol. 78: 265-71; discussion 271-2. PMID: 11745822
  4. 由 L 型氨基酸转运体 1（LAT1）介导的氨基酸相关化合物的转运：对底物识别机制的认识。  |  Uchino, H., et al. 2002. Mol Pharmacol. 61: 729-37. PMID: 11901210
  5. 人类 LAT1 单核苷酸多态性 N230K 不会改变苯丙氨酸的转运。  |  Boado, RJ., et al. 2004. Mol Genet Metab. 83: 306-11. PMID: 15589117
  6. 鉴定 S-亚硝基-L-半胱氨酸的立体选择性转运体：LAT1 和 LAT2 在 S-亚硝硫醇生物活性中的作用。  |  Li, S. and Whorton, AR. 2005. J Biol Chem. 280: 20102-10. PMID: 15769744
  7. 食管癌中 L 型氨基酸转运体 1 (LAT1) 的表达。  |  Kobayashi, H., et al. 2005. J Surg Oncol. 90: 233-8. PMID: 15906366
  8. L 型氨基酸转运体 1 在视网膜内血屏障介导的 L 型亮氨酸转运。  |  Tomi, M., et al. 2005. Invest Ophthalmol Vis Sci. 46: 2522-30. PMID: 15980244
  9. 人类 LAT1-4F2hc 异构氨基酸转运体复合物的结构。  |  Yan, R., et al. 2019. Nature. 568: 127-130. PMID: 30867591
  10. 氨基酸转运蛋白LAT1（SLC7A5）作为癌症诊断和治疗的分子靶标。  |  Kanai, Y. 2022. Pharmacol Ther. 230: 107964. PMID: 34390745
  11. 人类LAT1-4F2hc复合物的完整组装为研究其调节、功能和定位提供了新的视角。  |  Wu, D., et al. 2024. Nat Commun. 15: 3711. PMID: 38697966
  12. L 型氨基酸转运体参与大鼠海马中 S-亚硝基半胱氨酸刺激的去甲肾上腺素释放。  |  Satoh, S., et al. 1997. J Neurochem. 69: 2197-205. PMID: 9349567

订购信息

产品名称产品编号规格价格数量收藏夹

LAT1 抗体 (D-10)

sc-374232
200 µg/ml
RMB2377.00

LAT1 (D-10): m-IgG Fc BP-HRP 套装

sc-529694
200 µg Ab; 10 µg BP
RMB2662.00

LAT1 (D-10): m-IgGκ BP-HRP 套装

sc-522668
200 µg Ab, 40 µg BP
RMB2662.00

LAT1 (D-10): m-IgG1 BP-HRP 套装

sc-543638
200 µg Ab; 20 µg BP
RMB2662.00

LAT1 抗体 (D-10) AC

sc-374232 AC
500 µg/ml, 25% agarose
RMB3129.00

LAT1 抗体 (D-10) HRP

sc-374232 HRP
200 µg/ml
RMB2377.00

LAT1 抗体 (D-10) FITC

sc-374232 FITC
200 µg/ml
RMB2482.00

LAT1 抗体 (D-10) PE

sc-374232 PE
200 µg/ml
RMB2580.00

LAT1 抗体 (D-10) Alexa Fluor® 488

sc-374232 AF488
200 µg/ml
RMB2685.00

LAT1 抗体 (D-10) Alexa Fluor® 546

sc-374232 AF546
200 µg/ml
RMB2685.00

LAT1 抗体 (D-10) Alexa Fluor® 594

sc-374232 AF594
200 µg/ml
RMB2685.00

LAT1 抗体 (D-10) Alexa Fluor® 647

sc-374232 AF647
200 µg/ml
RMB2685.00

LAT1 抗体 (D-10) Alexa Fluor® 680

sc-374232 AF680
200 µg/ml
RMB2685.00

LAT1 抗体 (D-10) Alexa Fluor® 790

sc-374232 AF790
200 µg/ml
RMB2685.00

Good afternoon, I was wondering if you have any insights on detecting the LAT1 protein using your antibody by Western blot in RIPA-extracted mouse cardiac tissue, specifically around 25KDa?

Asked by: Sara
Thank you for your question. LAT1 Antibody (D-10): sc-374232 was tested and is recommended for detection of LAT1 of any mouse, rat, or human cell or tissue sample expressing the protein, by WB, IP, IF and ELISA. Observed molecular weight of LAT1 is 40 kDa; molecular weight of glycosylated LAT1 is 45-55 kDa.
Answered by: Tech Support Europe
Date published: 2025-03-26

Hello, which secondary antibodies would you recommend for IHC with this primary antibody? Which blocking agent would you recommend to use for IHC? Thank you

Asked by: Marie93
Thank you for your question. For HRP-based secondary detection of Anti-LAT1 Antibody (D-10): sc-374232 we recommend m-IgG1 BP-HRP: sc-525408. Our plant-based UltraCruz® Blocking Reagent: sc-516214 is recommended for blocking in IHC applications.
Answered by: Tech Support Europe
Date published: 2021-10-18

LAT1 Antibody (D-10): sc-374232 Hi, Can you please confirm that this antibody works for WB, IP, IF, ELISA and it is reactive against Human, Mouse and Rat? Thank you.

Asked by: Anonymous
Thank you for your question. As indicated on the product page, LAT1 Antibody (D-10): sc-374232 is recommended for detection of LAT1 of mouse, rat and human origin by WB, IP, IF and ELISA.
Answered by: Tech Support Europe
Date published: 2020-06-24

Hi, if this LAT1 antibody in conjugation with HRP is recommend by you for IHC(P) applications (citation: "HRP für WB, IHC(P) und ELISA"), which antigen retrieval method to your knowledge have been tested successfully for this antibody?

Asked by: Technical Support 2020
Thank you for your question. The HRP conjugated primary antibody is recommended and warrantied for immunohistochemistry on formalin-fixed paraffin-embedded tissues, or for IHC(P), however we are still gathering data to show on our website. Check back for updates! In general, we recommend using a sodium citrate buffer (pH 6.0) with heat treatment for antigen retrieval.
Answered by: Technical Support
Date published: 2020-04-08

I am interested in using this anti-body in a porcine model. Is there any data showing that the reagent can detect porcine?

Asked by: michdani
Thank you for your question. This antibody has not been tested for porcine use, and is not recommended or warrantied for this use. If you have any further questions, please contact Technical Service.
Answered by: Technical Support
Date published: 2018-12-11

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2017-01-13

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with LAT1 (D-10): sc-374232?

Asked by: jerojero
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2017-01-13
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Rated 1 out of 5 by from Variable/weak wb results from mouse CNS tissuesI've tried to use this antibody to detect Lat1 of mouse origin in cell lines and tissues from the brain and spinal cord, and I get variable signal. The protein is known to be expressed in my cells of interest, and the mRNA is really quite high as well. Either this antibody is less efficient for Ms Lat1, or its not specific. Based on this I wouldn't trust it for ICC unless using an over-expression model.
Date published: 2020-03-03
Rated 4 out of 5 by from Worked first tryGreat antibody worked for my in house cell lines at 1:1000. No extra bands very clean blot
Date published: 2017-08-01
Rated 5 out of 5 by from Excellent antibodyI received a sample for this antibody about a month ago. It gave me the results I was looking for. Highly specific antibody with little unspecific protein binding seen on my western blots.
Date published: 2017-04-24
Rated 5 out of 5 by from Outstanding !!Strong western blot data, low background; also superb IF staining
Date published: 2017-02-03
Rated 5 out of 5 by from Good in WB and stainingThis antibody shows strong signal for WB and staining in human samples.
Date published: 2017-01-11
Rated 5 out of 5 by from Did WesternDid Western, very good antibody
Date published: 2015-11-24
Rated 5 out of 5 by from Used in WBUsed in WB, good specificity.
Date published: 2015-05-26
Rated 5 out of 5 by from Produced excellent immunofluorescence cytoplasmicProduced excellent immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-15
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LAT1 抗体 (D-10) is rated 4.4 out of 5 by 9.
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