Date published: 2025-9-7

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Laminin γ-2 抗体 (E-6): sc-28330

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说明书
  • Laminin gamma-2 抗体 E-6 是小鼠单克隆 IgG1 κ,Laminin γ-2抗体, 在35篇文献中引用,规格为200 µg/ml
  • 免疫human物种的Laminin γ-2的氨基酸1011-1193
  • Laminin gamma-2 抗体 (E-6) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Laminin γ-2
  • 抗Laminin gamma-2抗体(E-6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Laminin γ-2 (E-6): sc-28330免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 Laminin γ-2 抗体 (E-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Laminin γ-2 抗体 (E-6) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

层粘连蛋白γ-2抗体(E-6)是一种小鼠单克隆IgG1κ轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人源的层粘连蛋白γ-2。抗层粘连蛋白γ-2抗体(E-6)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。层粘连蛋白γ-2在基底膜的形成和维持中发挥着关键作用,基底膜是一种特殊的细胞外基质,为组织提供结构支持,并影响细胞的增殖、迁移和分化等行为。这些基底膜含有Laminin γ-2,这是一种由α、β和γ链亚基组装而成的异三聚体。Laminin γ-2与各种细胞表面受体(包括整合素和肌动蛋白)相互作用,介导细胞粘附和信号传导,因此其适当的组装和功能对于组织的完整性和功能至关重要。人类Laminin γ-2基因位于1q25-q31染色体上,主要在皮肤、肺和肾脏的上皮细胞中表达,这凸显了其在维持这些组织的结构和功能完整性方面的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Laminin γ-2 抗体 (E-6) 参考文献:

  1. 层粘连蛋白异构体在肾小球结构中的作用。  |  Hansen, K. and Abrass, CK. 1999. Pathobiology. 67: 84-91. PMID: 10023136
  2. 形式与功能:层粘连蛋白异三聚体家族。  |  Colognato, H. and Yurchenco, PD. 2000. Dev Dyn. 218: 213-34. PMID: 10842354
  3. 层粘连蛋白基因表达的转录调控。  |  Aberdam, D., et al. 2000. Microsc Res Tech. 51: 228-37. PMID: 11054873
  4. 头颈部鳞状细胞癌中层粘连蛋白-γ2的过度表达。  |  Patel, V., et al. 2002. Int J Cancer. 99: 583-8. PMID: 11992550
  5. 层粘连蛋白γ2介导Wnt5a诱导的胃癌细胞侵袭。  |  Yamamoto, H., et al. 2009. Gastroenterology. 137: 242-52, 252.e1-6. PMID: 19582886
  6. 尿层粘连蛋白-γ2是非肌肉浸润性尿路上皮癌的一种新型生物标记物。  |  Kamada, M., et al. 2015. Cancer Sci. 106: 1730-7. PMID: 26450632
  7. amelotin, 层粘连蛋白γ2和牙本质发生相关磷蛋白(ODAPH)在基底层和交界上皮细胞上的表达和定位。  |  Li, C., et al. 2022. J Mol Histol. 53: 111-118. PMID: 34709488
  8. 层粘连蛋白家族  |  Tryggvason, K. 1993. Curr Opin Cell Biol. 5: 877-82. PMID: 8240830
  9. 层粘连蛋白在内皮细胞识别和分化中的作用  |  Schnaper, HW., et al. 1993. Kidney Int. 43: 20-5. PMID: 8433560
  10. 层粘连蛋白的结构域  |  Engvall, E. and Wewer, UM. 1996. J Cell Biochem. 61: 493-501. PMID: 8806072
  11. 层粘连蛋白与神经元生长机制  |  Luckenbill-Edds, L. 1997. Brain Res Brain Res Rev. 23: 1-27. PMID: 9063584
  12. 层粘连蛋白同工酶与上皮发育  |  Ekblom, M., et al. 1998. Ann N Y Acad Sci. 857: 194-211. PMID: 9917842

订购信息

产品名称产品编号规格价格数量收藏夹

Laminin γ-2 抗体 (E-6)

sc-28330
200 µg/ml
RMB2377.00

Laminin γ-2 (E-6): m-IgG Fc BP-HRP 套装

sc-526662
200 µg Ab; 10 µg BP
RMB2662.00

Laminin γ-2 (E-6): m-IgG1 BP-HRP 套装

sc-532035
200 µg Ab; 20 µg BP
RMB2662.00

Laminin γ-2 抗体 (E-6) AC

sc-28330 AC
500 µg/ml, 25% agarose
RMB3129.00

Laminin γ-2 抗体 (E-6) HRP

sc-28330 HRP
200 µg/ml
RMB2377.00

Laminin γ-2 抗体 (E-6) FITC

sc-28330 FITC
200 µg/ml
RMB2482.00

Laminin γ-2 抗体 (E-6) PE

sc-28330 PE
200 µg/ml
RMB2580.00

Laminin γ-2 抗体 (E-6) Alexa Fluor® 488

sc-28330 AF488
200 µg/ml
RMB2685.00

Laminin γ-2 抗体 (E-6) Alexa Fluor® 546

sc-28330 AF546
200 µg/ml
RMB2685.00

Laminin γ-2 抗体 (E-6) Alexa Fluor® 594

sc-28330 AF594
200 µg/ml
RMB2685.00

Laminin γ-2 抗体 (E-6) Alexa Fluor® 647

sc-28330 AF647
200 µg/ml
RMB2685.00

Laminin γ-2 抗体 (E-6) Alexa Fluor® 680

sc-28330 AF680
200 µg/ml
RMB2685.00

Laminin γ-2 抗体 (E-6) Alexa Fluor® 790

sc-28330 AF790
200 µg/ml
RMB2685.00

Can this Ab and sc-25341 (B-2) detect laminin subunit gamma-2 isoform b (NP_061486)?

Asked by: Mariya
Thank you for your question. Yes ,sc-25341(B-2) antibody can detect laminin subunit gamma-2 isoform b.
Answered by: BlakeJ
Date published: 2024-08-28

is this antibody suitable for IHC & IF against laminin 5 gamma 2?

Asked by: connectify
Thank you for your question. This antibody is recommended for the detection of Laminin γ-2 in many applications, including IF and IHC. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-08-30

I am using Laminin γ-2 (E-6): sc-28330 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Cweed
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Not for us.We used this sample on paraffin embedded-formalin-fixed-bladder but did not work at all for us. We went as high as 10ug/ml and got no signal.
Date published: 2017-02-14
Rated 5 out of 5 by from Excellent!This antibody is great in western blot analysis of Laminin γ-2 expression in A-431 whole cell lysate and mouse lung and rat lung tissue extracts.
Date published: 2017-01-27
Rated 5 out of 5 by from WB Published with this antibody using humanWB Published with this antibody using human skin cell samples. -SCBT Publication Review
Date published: 2015-04-14
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmicProduced excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human lung carcinoma tissue. -SCBT QC
Date published: 2015-02-25
Rated 4 out of 5 by from Nice antibody to use for Western BlotNice antibody to use for Western Blot, precise signal achieved in mouse lung and rat lung tissue extracts. -SCBT QC
Date published: 2015-01-17
Rated 5 out of 5 by from Publishable staining in paraffinPublishable staining in paraffin-embedded human esophagus tissue. -SCBT Publication Review
Date published: 2015-01-15
Rated 5 out of 5 by from IHC paraffin publishable data in normal humanIHC paraffin publishable data in normal human fibroblasts, E10 cells, cancer tissue, and the collagen matrix containing fibroblasts. -SCBT Publication Review
Date published: 2015-01-12
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Laminin γ-2 抗体 (E-6) is rated 4.3 out of 5 by 7.
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