Date published: 2025-9-7

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Laminin γ-1 抗体 (D-3): sc-17751

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说明书
  • Laminin γ-1抗体(D-3)是小鼠单克隆IgG1 κ, 在16篇文献中引用,规格为200 µg/ml
  • 免疫human物种的Laminin γ-1的氨基酸1420-1609
  • 抗-Laminin gamma-1 抗体 (D-3) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 Laminin γ-1
  • 抗Laminin gamma-1抗体(D-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Laminin γ-1 (D-3): sc-17751免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 Laminin γ-1 抗体 (D-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Laminin γ-1 抗体 (D-3) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

関連項目

层粘连蛋白γ-1抗体(D-3)是一种小鼠单克隆IgG1κ轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和免疫组织化学检测小鼠、大鼠和人类来源的层粘连蛋白γ-1。抗层粘连蛋白γ-1抗体(D-3)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。层粘连蛋白(包括层粘连蛋白γ-1)是基底膜的重要结构成分,基底膜是一种特殊的细胞外基质,为细胞提供支撑和锚定。这些膜不仅对维持组织结构至关重要,而且对调节细胞行为(如增殖、迁移和分化)也起着重要作用。层粘连蛋白的独特结构由α、β和γ链组成的异三聚体组成,使其能够自我组装并与其他基质成分相互作用,促进稳定基底膜的形成。人类层粘连蛋白γ-1基因位于1q31号染色体上,在多种组织中表达,这凸显了其在需要强健细胞外基质的组织发育和维持中的重要性。层粘连蛋白γ-1(D-3)单克隆抗体是研究人员研究基底膜动态及其对健康和疾病中细胞功能的影响的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Laminin γ-1 抗体 (D-3) 参考文献:

  1. bcn-1 转录因子 E3(TFE3)和 Smad 蛋白的合作作用对层粘蛋白 gamma 1 链基因的依赖性激活。  |  Kawata, Y., et al. 2002. J Biol Chem. 277: 11375-84. PMID: 11801598
  2. 在CNS轴突再生过程中,基底膜非依赖性层粘连蛋白γ1链起着关键作用。  |  Grimpe, B., et al. 2002. J Neurosci. 22: 3144-60. PMID: 11943817
  3. 层粘蛋白γ1链肽C-16(KAFDITYVRLKF)促进B16-F10小鼠黑色素瘤细胞的迁移、MMP-9分泌和肺转移  |  Kuratomi, Y., et al. 2002. Br J Cancer. 86: 1169-73. PMID: 11953867
  4. Plasmin 介导的层粘连蛋白 gamma-1 降解对乙醇诱导的神经退行性病变至关重要  |  Skrzypiec, AE., et al. 2009. Biol Psychiatry. 66: 785-94. PMID: 19577227
  5. 与银屑病红斑相关的表皮下大疱性皮肤病中针对Bp180和层粘蛋白γ-1中多个表位的自身抗体。  |  Goto-Hamano, H., et al. 2015. Indian J Dermatol. 60: 521. PMID: 26538726
  6. 层粘连蛋白γ1链降解的血清学生物标记反映了克罗恩病和DSS结肠炎基底膜重塑的改变。  |  Lindholm, M., et al. 2022. Dig Dis Sci. 67: 3662-3671. PMID: 34561759
  7. 少突胶质细胞源性层粘连蛋白-γ1在小鼠中调节血脑屏障和中枢神经系统髓鞘化。  |  Kang, M. and Yao, Y. 2024. Cell Rep. 43: 114123. PMID: 38635399
  8. 两个非连续区域有助于nidogen与层粘连蛋白γ1链的单个EGF样结构结合。  |  Pöschl, E., et al. 1994. EMBO J. 13: 3741-7. PMID: 8070402
  9. 培养肝细胞中层粘连蛋白γ1的表达涉及LAMC1启动子中重复的CTC和GC元件。  |  Levavasseur, F., et al. 1996. Biochem J. 313 (Pt 3): 745-52. PMID: 8611150
  10. 大鼠层粘连蛋白γ1-链基因启动子的克隆揭示了多种转录因子的识别基团。  |  O'Neill, BC., et al. 1997. Am J Physiol. 273: F411-20. PMID: 9321914

订购信息

产品名称产品编号规格价格数量收藏夹

Laminin γ-1 抗体 (D-3)

sc-17751
200 µg/ml
RMB2377.00

Laminin γ-1 (D-3): m-IgG Fc BP-HRP 套装

sc-528295
200 µg Ab; 10 µg BP
RMB2662.00

Laminin γ-1 (D-3): m-IgGκ BP-HRP 套装

sc-520636
200 µg Ab, 40 µg BP
RMB2662.00

Laminin γ-1 (D-3): m-IgG1 BP-HRP 套装

sc-542856
200 µg Ab; 20 µg BP
RMB2662.00

Laminin γ-1 抗体 (D-3) AC

sc-17751 AC
500 µg/ml, 25% agarose
RMB3129.00

Laminin γ-1 抗体 (D-3) HRP

sc-17751 HRP
200 µg/ml
RMB2377.00

Laminin γ-1 抗体 (D-3) FITC

sc-17751 FITC
200 µg/ml
RMB2482.00

Laminin γ-1 抗体 (D-3) PE

sc-17751 PE
200 µg/ml
RMB2580.00

Laminin γ-1 抗体 (D-3) Alexa Fluor® 488

sc-17751 AF488
200 µg/ml
RMB2685.00

Laminin γ-1 抗体 (D-3) Alexa Fluor® 546

sc-17751 AF546
200 µg/ml
RMB2685.00

Laminin γ-1 抗体 (D-3) Alexa Fluor® 594

sc-17751 AF594
200 µg/ml
RMB2685.00

Laminin γ-1 抗体 (D-3) Alexa Fluor® 647

sc-17751 AF647
200 µg/ml
RMB2685.00

Laminin γ-1 抗体 (D-3) Alexa Fluor® 680

sc-17751 AF680
200 µg/ml
RMB2685.00

Laminin γ-1 抗体 (D-3) Alexa Fluor® 790

sc-17751 AF790
200 µg/ml
RMB2685.00

I am using Laminin γ-1 (D-3): sc-17751 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great signal strengthUsed a 1:300 dilution for WB with Sum159, Caco-2, and 293T cell lysates. Great signal strength. A second band was present at 50 kDa
Date published: 2017-11-30
Rated 5 out of 5 by from Excellent antibodyThe Laminin gamma-1 Antibody (D-3): sc-17751 provides great staining data for IHC with formalin fixed, paraffin-embedded tissue sections, showing cytoplasmic and extracellular localization.
Date published: 2017-01-23
Rated 5 out of 5 by from Great signal in WB against HeLa whole cell lysateGreat signal in WB against HeLa whole cell lysate. -SCBT QC
Date published: 2015-03-10
Rated 5 out of 5 by from Good WB publishable data gathered with humanGood WB publishable data gathered with human skin organotypic cocultures. -SCBT Publication Review
Date published: 2015-03-02
Rated 5 out of 5 by from Good data with paraffinGood data with paraffin-embedded human tumor samples. -SCBT Publication Review
Date published: 2015-03-02
Rated 4 out of 5 by from Works well in WB against AWorks well in WB against A-431 whole cell lysate. -SCBT QC
Date published: 2015-02-23
Rated 5 out of 5 by from Produced superb immunofluorescence trabecularProduced superb immunofluorescence trabecular meshwork staining in normal human cornea frozen section. -SCBT QC
Date published: 2015-01-30
Rated 5 out of 5 by from Published data in human fibroblasts usingPublished data in human fibroblasts using this antibody for Western blot analysis -SCBT Publication Review
Date published: 2015-01-15
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Laminin γ-1 抗体 (D-3) is rated 4.9 out of 5 by 8.
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