Date published: 2025-9-7

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Laminin β-3 抗体 (A-6): sc-133178

4.3(6)
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说明书
  • Laminin beta-3 抗体 A-6 是小鼠单克隆 IgG1 κ,Laminin β-3抗体, 在11篇文献中引用,规格为200 µg/ml
  • 针对氨基酸873-1172,在Laminin β-3的human起源的C-terminus处进行映射
  • Laminin beta-3 抗体 (A-6) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 Laminin β-3
  • 抗Laminin beta-3抗体(A-6)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Laminin β-3 (A-6): sc-133178免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 Laminin β-3 抗体 (A-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Laminin β-3 抗体 (A-6) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

层粘连蛋白β-3抗体(A-6)是一种IgG1κ小鼠单克隆层粘连蛋白β-3抗体(也称为LAMB3抗体、LAMNB1抗体、层粘连蛋白β3抗体、Kalinin B1抗体或Epiligrin亚基β抗体),可通过WB、IP、IF、IHC(P)和ELISA检测人源的层粘连蛋白β-3蛋白。层粘连蛋白β-3抗体(A-6)既可作为非偶联的抗层粘连蛋白β-3抗体形式,也可作为多种偶联形式的抗层粘连蛋白β-3抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。层粘连蛋白由一个由三条基因不同的多肽链组成的二硫键连接的异三聚体家族组成,称为α、β和γ。作为基底膜的主要成分,层粘连蛋白在提供组织组装所需的支架以及作为分离特殊组织的物理屏障方面起着至关重要的作用。在胚胎发生和早期发育过程中,细胞沿着基底膜迁移,这是细胞极化所必需的。已描述至少八种层粘连蛋白同工型:α-1、α-2、α-3、β-1、β-2、β-3、γ-1和γ-2。每种同工型与单个层粘连蛋白受体的相对亲和力不同。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Laminin β-3 抗体 (A-6) 参考文献:

  1. 胃癌中层粘连蛋白β3和γ2链的共表达以及层粘连蛋白α3链的表观遗传失活。  |  Ii, M., et al. 2011. Int J Oncol. 39: 593-9. PMID: 21617852
  2. microRNA-329抑制胆管癌上皮细胞向间充质细胞转化和淋巴结转移,其作用机制是抑制层粘连蛋白亚基β3。  |  Liao, CH., et al. 2019. J Cell Physiol. 234: 17786-17799. PMID: 30887508
  3. 子宫内膜层粘连蛋白亚基β-3的表达与反复植入失败患者的生殖结果有关。  |  Li, T., et al. 2021. J Assist Reprod Genet. 38: 1835-1842. PMID: 33715134
  4. 甲状腺素对星形胶质细胞整合素-层粘连相互作用的依赖性调节  |  Farwell, AP., et al. 1995. Endocrinology. 136: 3909-15. PMID: 7649099
  5. 层粘连蛋白  |  Timpl, R. and Brown, JC. 1994. Matrix Biol. 14: 275-81. PMID: 7827749
  6. 基底层组装  |  Yurchenco, PD. and O'Rear, JJ. 1994. Curr Opin Cell Biol. 6: 674-81. PMID: 7833047
  7. 层粘连蛋白:基底膜上多种多样的多功能分子家族。  |  Aumailley, M. and Krieg, T. 1996. J Invest Dermatol. 106: 209-214. PMID: 8601717
  8. 层粘连蛋白链组装成三链盘卷结构的机制。  |  Nomizu, M., et al. 1996. Biochemistry. 35: 2885-93. PMID: 8608125
  9. 培养的人肝星状细胞中胶原蛋白和层粘连蛋白整合素受体的表达和功能。  |  Carloni, V., et al. 1996. Gastroenterology. 110: 1127-36. PMID: 8613002
  10. 层粘连蛋白与基底膜的相互作用对基底膜的组装非常重要,锌促进了层粘连蛋白的锌指样序列。  |  Ancsin, JB. and Kisilevsky, R. 1996. J Biol Chem. 271: 6845-51. PMID: 8636109
  11. 基底膜的结构和功能。  |  Engvall, E. 1995. Int J Dev Biol. 39: 781-7. PMID: 8645562
  12. 肿瘤进展和转移过程中的层粘连整合素。  |  Ziober, BL., et al. 1996. Semin Cancer Biol. 7: 119-28. PMID: 8773297

订购信息

产品名称产品编号规格价格数量收藏夹

Laminin β-3 抗体 (A-6)

sc-133178
200 µg/ml
RMB2377.00

Laminin β-3 (A-6): m-IgG Fc BP-HRP 套装

sc-528761
200 µg Ab; 10 µg BP
RMB2662.00

Laminin β-3 (A-6): m-IgGκ BP-HRP 套装

sc-521265
200 µg Ab, 40 µg BP
RMB2662.00

Laminin β-3 (A-6): m-IgG1 BP-HRP 套装

sc-543116
200 µg Ab; 20 µg BP
RMB2662.00

Laminin β-3 抗体 (A-6) AC

sc-133178 AC
500 µg/ml, 25% agarose
RMB3129.00

Laminin β-3 抗体 (A-6) HRP

sc-133178 HRP
200 µg/ml
RMB2377.00

Laminin β-3 抗体 (A-6) FITC

sc-133178 FITC
200 µg/ml
RMB2482.00

Laminin β-3 抗体 (A-6) PE

sc-133178 PE
200 µg/ml
RMB2580.00

Laminin β-3 抗体 (A-6) Alexa Fluor® 488

sc-133178 AF488
200 µg/ml
RMB2685.00

Laminin β-3 抗体 (A-6) Alexa Fluor® 546

sc-133178 AF546
200 µg/ml
RMB2685.00

Laminin β-3 抗体 (A-6) Alexa Fluor® 594

sc-133178 AF594
200 µg/ml
RMB2685.00

Laminin β-3 抗体 (A-6) Alexa Fluor® 647

sc-133178 AF647
200 µg/ml
RMB2685.00

Laminin β-3 抗体 (A-6) Alexa Fluor® 680

sc-133178 AF680
200 µg/ml
RMB2685.00

Laminin β-3 抗体 (A-6) Alexa Fluor® 790

sc-133178 AF790
200 µg/ml
RMB2685.00

Does this antibody work for immunocytochemistry when PFA (4%) and triton (0.5%) have been worked for fix and permeabilize the cells, respectively?

Asked by: Josy123
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2023-03-21

Ist der AK cryogängig? Dann würden wir ihn bestellen.

Asked by: Atefi
Vielen Dank für Ihre Anfrage. Der Laminin beta-3 Antikörper (A-6) ist für die Detektion von humanem Laminin β-3 mittels WB, IP, IF, IHC(P) und ELISA geeignet. Diese Empfehlung schließt auch die Anwendung in Cryoschnitten ein.
Answered by: Tech Support Europe
Date published: 2021-08-31

I am using Laminin β-3 (A-6): sc-133178 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2024-10-10
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Rated 1 out of 5 by from schwachTestsample erhalten, Probefärbungen durchgeführt, für unsere Zwecke nicht einsetzbar
Date published: 2017-08-28
Rated 5 out of 5 by from Clean bands with backgroundLaminin β-3 antibody works very well for WB showing clear band with minimal background. This antibody has clean bands with background.
Date published: 2017-02-04
Rated 5 out of 5 by from Good western blot resultsWe used this antibody for western blot and got a positive band for the correct molecular weight
Date published: 2017-01-17
Rated 5 out of 5 by from Positive result in WB in SCCPositive result in WB in SCC-4 whole cell lysate. -SCBT QC
Date published: 2015-04-03
Rated 5 out of 5 by from Good signal for WB in human LamininGood signal for WB in human Laminin -3 transfected 293T and A-431 whole cell lysates. -SCBT QC
Date published: 2015-02-20
Rated 5 out of 5 by from Produced exceptional immunoperoxidase cytoplasmicProduced exceptional immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human placenta tissue. -SCBT QC
Date published: 2015-01-13
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Laminin β-3 抗体 (A-6) is rated 4.3 out of 5 by 6.
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