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Laminin β-1 Antibody (LT3) - Western Blotting - Image 380895
层粘连蛋白 β-1 抗体 (LT3): sc-33709. NIH/3T3 (A) 和 EOC 20 (B) 全细胞裂解液中层粘连蛋白 β-1 表达的 Western 印迹分析.
层粘连蛋白 β-1 抗体 (LT3): sc-33709. NIH/3T3 (A) 和 EOC 20 (B) 全细胞裂解液中层粘连蛋白 β-1 表达的 Western 印迹分析.

Laminin β-1 抗体 (LT3): sc-33709

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说明书
  • Laminin beta-1 抗体 LT3 是大鼠单克隆 IgG1, 在11篇文献中引用，提供 200 µg/ml
  • 针对部分纯化的EHS小鼠肿瘤层粘连蛋白制剂
  • 抗-Laminin beta-1 抗体 (LT3) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 Laminin β-1
  • 抗Laminin beta-1抗体（LT3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Laminin β-1 (LT3): sc-33709的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成Laminin β-1 抗体 (LT3)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Laminin 抗体 包括 Laminin-5, Laminin α-1, Laminin α-2, Laminin α-4, Laminin α-5, Laminin β-1, Laminin β-2, Laminin β-3, Laminin γ-1和Laminin γ-2

层粘连蛋白β-1抗体（LT3）是一种鼠单克隆IgG1抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学等方法检测小鼠、大鼠和人类样本中的层粘连蛋白β-1。抗层粘连蛋白β-1抗体（LT3）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。层粘连蛋白β-1在基底膜的形成和维持中起着至关重要的作用，基底膜是一种特殊的细胞外基质，为组织提供结构支持，并影响细胞的增殖、迁移和分化等行为。这些基底膜由层粘连蛋白组成，层粘连蛋白是一种由α、β和γ链组成的异三聚体蛋白，与各种细胞表面受体（包括整合素和肌动蛋白）相互作用，以介导细胞粘附和信号传导。基底膜的正常组装和功能对于组织的完整性和修复至关重要，因为它们是细胞的屏障和支架。人类层粘连蛋白β-1基因位于7q22染色体上，在多种组织中表达，这凸显了其在正常生理和病理条件下的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Laminin β-1 抗体 (LT3) 参考文献:

  1. 层粘连蛋白异构体在肾小球结构中的作用。  |  Hansen, K. and Abrass, CK. 1999. Pathobiology. 67: 84-91. PMID: 10023136
  2. 形式与功能：层粘连蛋白异三聚体家族。  |  Colognato, H. and Yurchenco, PD. 2000. Dev Dyn. 218: 213-34. PMID: 10842354
  3. 层粘连蛋白基因表达的转录调控。  |  Aberdam, D., et al. 2000. Microsc Res Tech. 51: 228-37. PMID: 11054873
  4. 层粘连蛋白β1通过抑制ERK/MAPK和SGK1信号传导来损害空间学习。  |  Yang, YC., et al. 2011. Neuropsychopharmacology. 36: 2571-86. PMID: 21849984
  5. 层粘连蛋白β1和γ1链的短臂形成网络的晶体结构。  |  Carafoli, F., et al. 2012. PLoS One. 7: e42473. PMID: 22860131
  6. 层粘连蛋白β1链的磷酸化图谱：与活性位点相关的激酶。  |  Verrou, KM., et al. 2019. J Biosci. 44: PMID: 31180068
  7. CRISPR-Cas9介导的人类层粘连蛋白β1 C末端标记导致分泌抑制。  |  Shaw, L., et al. 2020. BMC Res Notes. 13: 90. PMID: 32085798
  8. 层粘连蛋白家族  |  Tryggvason, K. 1993. Curr Opin Cell Biol. 5: 877-82. PMID: 8240830
  9. 层粘连蛋白在内皮细胞识别和分化中的作用  |  Schnaper, HW., et al. 1993. Kidney Int. 43: 20-5. PMID: 8433560
  10. 层粘连蛋白的结构域  |  Engvall, E. and Wewer, UM. 1996. J Cell Biochem. 61: 493-501. PMID: 8806072
  11. 层粘连蛋白与神经元生长机制  |  Luckenbill-Edds, L. 1997. Brain Res Brain Res Rev. 23: 1-27. PMID: 9063584
  12. 层粘连蛋白同工酶与上皮发育  |  Ekblom, M., et al. 1998. Ann N Y Acad Sci. 857: 194-211. PMID: 9917842

订购信息

产品名称产品编号规格价格数量收藏夹

Laminin β-1 抗体 (LT3)

sc-33709
200 µg/ml
RMB2377.00

Laminin β-1 抗体 (LT3) AC

sc-33709 AC
500 µg/ml, 25% agarose
RMB3129.00

Laminin β-1 抗体 (LT3) HRP

sc-33709 HRP
200 µg/ml
RMB2377.00

Laminin β-1 抗体 (LT3) FITC

sc-33709 FITC
200 µg/ml
RMB2482.00

Laminin β-1 抗体 (LT3) PE

sc-33709 PE
200 µg/ml
RMB2580.00

Laminin β-1 抗体 (LT3) Alexa Fluor® 488

sc-33709 AF488
200 µg/ml
RMB2685.00

Laminin β-1 抗体 (LT3) Alexa Fluor® 546

sc-33709 AF546
200 µg/ml
RMB2685.00

Laminin β-1 抗体 (LT3) Alexa Fluor® 594

sc-33709 AF594
200 µg/ml
RMB2685.00

Laminin β-1 抗体 (LT3) Alexa Fluor® 647

sc-33709 AF647
200 µg/ml
RMB2685.00

Laminin β-1 抗体 (LT3) Alexa Fluor® 680

sc-33709 AF680
200 µg/ml
RMB2685.00

Laminin β-1 抗体 (LT3) Alexa Fluor® 790

sc-33709 AF790
200 µg/ml
RMB2685.00

Dear sir/madam, I would like to get a free sample of the Laminin β-1 Antibody (LT3): sc-33709. Thanks in advance, Christa Tanganyika

Asked by: christa
Thank you for your question. If you have an interest in testing a free 10 µg sample of sc-33709, please contact our Technical Service by phone or email or by Live Chat.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-21

Which product is recommended for kidney regeneration?

Asked by: Rupert13
Thank you for your question. Unfortunately we are not able to provide that information. Our antibodies are for research use only. If you have any further questions, please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-04-14

What is the recommended fixation method for immunofluorescence staining with Laminin β-1 (LT3): sc-33709 monoclonal antibody?

Asked by: DefinitelyNotMatt
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from IF do not work on frozen sectionsTested IF on frozen sections of 4% PFA fixed mouse cerebellum, no specific signal was detected.
Date published: 2018-07-30
Rated 4 out of 5 by from Great Western blot data of LamininWe used this antibody to detect laminin beta 1 and got a good result. Fresh liver samples were homogenized in RIPA buffer, then 40ug of total protein were subjected to 10% gel for SDS-PAGE and transferred onto a PVDF membrane. The membrane were incubated overnight at 4℃ with laminin beta 1 antibodies(1:1000) and it were incubated with anti- rat IgG(1:2000) at room temperature and detected with ECL kit.
Date published: 2017-06-07
Rated 5 out of 5 by from Works wellThis antibody performs well in staining and blotting procedures. Signal is clear and specific and will help you achieve your desired outcome.
Date published: 2017-02-07
Rated 4 out of 5 by from Seems to work well in our Western but weSeems to work well in our Western but we have not fully tested.
Date published: 2015-07-28
Rated 5 out of 5 by from Produced superb immunofluorescence basementProduced superb immunofluorescence basement membrane, Descemet s membrane and smooth muscle staining in normal mouse eye frozen section. -SCBT QC
Date published: 2015-05-10
Rated 5 out of 5 by from Strong signal for WB in T98G whole cell lysateStrong signal for WB in T98G whole cell lysate. -SCBT QC
Date published: 2015-04-27
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Laminin β-1 抗体 (LT3) is rated 4.0 out of 5 by 6.
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