Date published: 2025-9-7
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Laminin α-1 Antibody (G-12) - Western Blotting - Image 15609
层粘蛋白 α-1 (G-12): sc-74418. Caki-1 全细胞裂解液中层粘蛋白 α-1 表达的 Western 印迹分析.
Laminin α-1 Antibody (G-12) - Western Blotting - Image 15609
层粘蛋白 α-1 (G-12): sc-74418. Caki-1 全细胞裂解液中层粘蛋白 α-1 表达的 Western 印迹分析.
Laminin α-1 Antibody (G-12) - Western Blotting - Image 400930
层粘蛋白 α-1 抗体 (G-12): sc-74418. Caki-1 全细胞裂解液 (A) 和人睾丸组织提取物 (B) 中层粘连蛋白 α-1 表达的 Western 印迹分析. 所用检测试剂: m-IgG1 BP-HRP: sc-525408.
层粘蛋白 α-1 (G-12): sc-74418. Caki-1 全细胞裂解液中层粘蛋白 α-1 表达的 Western 印迹分析.

Laminin α-1 抗体 (G-12): sc-74418

4.5(6)
寫評論提問

说明书
  • Laminin alpha-1 抗体 G-12 是小鼠单克隆 IgG1 κ，Laminin α-1抗体, 在9篇文献中引用，规格为200 µg/ml
  • 针对氨基酸1856-2099的图谱，在Laminin α-1的内部区域，human起源
  • Laminin alpha-1 抗体 (G-12) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 Laminin α-1
  • 抗Laminin alpha-1抗体（G-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Laminin α-1 (G-12): sc-74418的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是Laminin α-1 Antibody (G-12) for WB applications. 的首选辅助检测试剂，这些试剂现与Laminin α-1 Antibody (G-12) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加Laminin抗体，包括Laminin-5, Laminin α-1, Laminin α-2, Laminin α-4, Laminin α-5, Laminin β-1, Laminin β-2, Laminin β-3, Laminin γ-1, Laminin γ-2和Laminin γ-3

层粘连蛋白α-1抗体（G-12）是一种小鼠单克隆IgG1κ轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测人类源性层粘连蛋白α-1蛋白。层粘连蛋白α-1 (G-12) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。层粘连蛋白α-1是基底膜的重要组成部分，基底膜是一种特殊的细胞外基质，为组织提供结构支持，在细胞信号传导和组织结构中发挥着重要作用。这些膜主要位于上皮细胞下方、血管周围以及肌肉和神经细胞的外膜，从而影响细胞粘附、迁移和分化等过程。层粘连蛋白的独特结构由三条多肽链（α、β和γ）组成，能够形成与其他细胞外基质成分相互作用的网状结构，促进基底膜的组装。层粘连蛋白α-1与多种病理状况有关，包括阿尔茨海默病患者额叶皮层中的过度表达，这表明其在神经退行性病变中可能具有潜在作用。人类层粘连蛋白α-1基因位于18p11.3染色体上，了解其功能和相互作用对于阐明其对正常生理和疾病状态的影响至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Laminin α-1 抗体 (G-12) 参考文献:

  1. 层粘连蛋白异构体在肾小球结构中的作用。  |  Hansen, K. and Abrass, CK. 1999. Pathobiology. 67: 84-91. PMID: 10023136
  2. 结肠癌细胞系中的层粘连蛋白α链：在Caco-2细胞中检测到截短的层粘连蛋白α1 mRNA。  |  Velling, T., et al. 1999. Exp Cell Res. 248: 627-33. PMID: 10222155
  3. 形式与功能：层粘连蛋白异三聚体家族。  |  Colognato, H. and Yurchenco, PD. 2000. Dev Dyn. 218: 213-34. PMID: 10842354
  4. 层粘连蛋白基因表达的转录调控。  |  Aberdam, D., et al. 2000. Microsc Res Tech. 51: 228-37. PMID: 11054873
  5. 鉴定层粘连蛋白α链N端结构域上具有生物活性的同源位点。  |  Nomizu, M., et al. 2001. Biochemistry. 40: 15310-7. PMID: 11735413
  6. 层粘连蛋白α链LG模块中同源环区的生物学活性。  |  Katagiri, F., et al. 2014. Biochemistry. 53: 3699-708. PMID: 24850085
  7. 层粘连蛋白α和β亚基的差异蛋白水解敏感性。  |  Rao, CN., et al. 1982. Arch Biochem Biophys. 219: 65-70. PMID: 6758704
  8. 层粘连蛋白家族  |  Tryggvason, K. 1993. Curr Opin Cell Biol. 5: 877-82. PMID: 8240830
  9. 层粘连蛋白在内皮细胞识别和分化中的作用  |  Schnaper, HW., et al. 1993. Kidney Int. 43: 20-5. PMID: 8433560
  10. 层粘连蛋白的结构域  |  Engvall, E. and Wewer, UM. 1996. J Cell Biochem. 61: 493-501. PMID: 8806072
  11. 层粘连蛋白与神经元生长机制  |  Luckenbill-Edds, L. 1997. Brain Res Brain Res Rev. 23: 1-27. PMID: 9063584
  12. 层粘连蛋白同工酶与上皮发育  |  Ekblom, M., et al. 1998. Ann N Y Acad Sci. 857: 194-211. PMID: 9917842

订购信息

产品名称产品编号规格价格数量收藏夹

Laminin α-1 抗体 (G-12)

sc-74418
200 µg/ml
RMB2377.00

Laminin α-1 (G-12): m-IgG Fc BP-HRP 套装

sc-527018
200 µg Ab; 10 µg BP
RMB2662.00

Laminin α-1 (G-12): m-IgG1 BP-HRP 套装

sc-532391
200 µg Ab; 20 µg BP
RMB2662.00

Laminin α-1 抗体 (G-12) AC

sc-74418 AC
500 µg/ml, 25% agarose
RMB3129.00

Laminin α-1 抗体 (G-12) HRP

sc-74418 HRP
200 µg/ml
RMB2377.00

Laminin α-1 抗体 (G-12) FITC

sc-74418 FITC
200 µg/ml
RMB2482.00

Laminin α-1 抗体 (G-12) PE

sc-74418 PE
200 µg/ml
RMB2580.00

Laminin α-1 抗体 (G-12) Alexa Fluor® 488

sc-74418 AF488
200 µg/ml
RMB2685.00

Laminin α-1 抗体 (G-12) Alexa Fluor® 546

sc-74418 AF546
200 µg/ml
RMB2685.00

Laminin α-1 抗体 (G-12) Alexa Fluor® 594

sc-74418 AF594
200 µg/ml
RMB2685.00

Laminin α-1 抗体 (G-12) Alexa Fluor® 647

sc-74418 AF647
200 µg/ml
RMB2685.00

Laminin α-1 抗体 (G-12) Alexa Fluor® 680

sc-74418 AF680
200 µg/ml
RMB2685.00

Laminin α-1 抗体 (G-12) Alexa Fluor® 790

sc-74418 AF790
200 µg/ml
RMB2685.00

Please suggest me a paper using this antibody for IHC in human tissue.

Asked by: Siwanon
Unfortunately we are not aware of a citation using this antibody for IF at this time. Please contact your local distributor or technical service if you have any further questions.
Answered by: Technical Support
Date published: 2019-01-06

What blocking solution should I use with Laminin α-1 (G-12): sc-74418 monoclonal antibody for Western blot?

Asked by: TinTin
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-13
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Rated 3 out of 5 by from not work in zebrafish.i tried a zebrafish whole mount IF with a small sample package, unfortunately, this antibody can't work in zebrafish, no signals.
Date published: 2018-04-24
Rated 5 out of 5 by from very nice staining by immunofluorescencework very well when diluted 1/300 on paraffin sections from adult testis
Date published: 2017-12-04
Rated 4 out of 5 by from good for western blot analysisWe used this antibody to detect Smo and got a good result. Fresh HUVEC were homogenized in RIPA buffer, then 20ug of total protein were subjected to 10% gel for SDS-PAGE and transferred onto a PVDF membrane. After blocking with 5% skim milk in 1×TBS-T, membrane were incubated overnight at 4℃ with Smo antibodies(1:1000).And then, it were incubated with anti- mouse IgG(1:2000) at room temperature and detected with ECL kit.
Date published: 2017-06-07
Rated 5 out of 5 by from good3 months ago, I used this antibody for immunohistochemistry (paraffin embedded tissue). The effect is very good, very specific, no background staining. EDTA 8 heat antigen repair.
Date published: 2017-05-11
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of Laminin α-1 expression in Caki-1 whole cell lysate.
Date published: 2017-02-07
Rated 5 out of 5 by from Great Western blot data of LamininGreat Western blot data of Laminin -1 expression in Caki-1 whole cell lysate. -SCBT QC
Date published: 2013-06-03
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Laminin α-1 抗体 (G-12) is rated 4.5 out of 5 by 6.
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