Date published: 2025-9-8

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Lambda 5 抗体 (A-1): sc-398932

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  • Lambda 5 抗体 A-1 是小鼠单克隆 IgG2a (kappa轻链),Lambda 5抗体,规格为200 µg/ml
  • 免疫原氨基酸序列38-97位于human物种的Lambda 5的N-terminus附近
  • Lambda 5 抗体 (A-1) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Lambda 5
  • 抗Lambda 5抗体(A-1)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Lambda 5 (A-1): sc-398932免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP是Lambda 5 Antibody (A-1) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Lambda 5 Antibody (A-1) 打包提供(请参阅下面的订购信息)。

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    関連項目

    Lambda 5抗体(A-1)是一种IgG2a κ小鼠单克隆Lambda 5抗体(也称为Lambda 5抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的Lambda 5蛋白。Lambda 5抗体(A-1)既可以是非偶联的抗Lambda 5抗体形式,也可以是多种偶联形式的抗Lambda 5抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Lambda 5(也称为免疫球蛋白λ样多肽1或CD179b抗原)和VpreB构成前B细胞受体复合物的替代轻链。SL链也是质量控制机制的一部分,该机制测试m链与常规L链配对的能力。它可以与重(H)链、DHJHC m蛋白或p55链形成类似Ig的复合物。替代轻链的产生始于前B细胞阶段,在前B细胞阶段继续,并在未成熟B细胞阶段停止。一旦表达前BCR,SL链表达就会被关闭。随着前B II细胞的增殖,SL被稀释,从而限制了前BCR的形成。Lambda 5对哺乳动物B细胞的发育至关重要。Lambda 5在肝脏、前B淋巴细胞和骨髓中的表达最高,骨髓是B细胞前体的主要来源。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    Lambda 5 抗体 (A-1) 参考文献:

    1. 转基因人λ5能挽救小鼠λ5的无效表型。  |  Donohoe, ME., et al. 2000. J Immunol. 164: 5269-76. PMID: 10799888
    2. 小鼠前体 B 细胞受体与基质细胞的相互作用受控于 lambda 5 的独特尾部和基质细胞相关的硫酸肝素。  |  Bradl, H., et al. 2003. J Immunol. 171: 2338-48. PMID: 12928380
    3. 前沿:在没有λ5的情况下μH链的信号传导和细胞表面表达:范例再探。  |  Schuh, W., et al. 2003. J Immunol. 171: 3343-7. PMID: 14500626
    4. 在人类前 B 细胞中构成 mu-psi 轻链复合物的 lambda 样基因的组织和表达。  |  Bossy, D., et al. 1991. Int Immunol. 3: 1081-90. PMID: 1760403
    5. 免疫球蛋白λ轻链相关基因 14.1 和 16.1 在前 B 细胞中表达,可能编码人类免疫球蛋白Ω轻链蛋白。  |  Hollis, GF., et al. 1989. Proc Natl Acad Sci U S A. 86: 5552-6. PMID: 2501791
    6. c-myc 蛋白抑制了 lambda 5 和 TdT 启动子。  |  Mai, S. and Mårtensson, IL. 1995. Nucleic Acids Res. 23: 1-9. PMID: 7870572
    7. 表达重链疾病蛋白的转基因小鼠在缺乏λ5的情况下的前B细胞发育。  |  Corcos, D., et al. 1995. Curr Biol. 5: 1140-8. PMID: 8548286
    8. 人类 lambda5/14.1 基因突变会导致 B 细胞缺乏症和传染性球蛋白血症。  |  Minegishi, Y., et al. 1998. J Exp Med. 187: 71-7. PMID: 9419212

    订购信息

    产品名称产品编号规格价格数量收藏夹

    Lambda 5 抗体 (A-1)

    sc-398932
    200 µg/ml
    RMB2377.00

    Lambda 5 (A-1): m-IgG Fc BP-HRP 套装

    sc-530979
    200 µg Ab; 10 µg BP
    RMB2662.00

    Lambda 5 (A-1): m-IgGκ BP-HRP 套装

    sc-524546
    200 µg Ab, 40 µg BP
    RMB2662.00

    Lambda 5 (A-1): m-IgG2a BP-HRP 套装

    sc-547650
    200 µg Ab; 10 µg BP
    RMB2662.00

    Lambda 5 抗体 (A-1) AC

    sc-398932 AC
    500 µg/ml, 25% agarose
    RMB3129.00

    Lambda 5 抗体 (A-1) HRP

    sc-398932 HRP
    200 µg/ml
    RMB2377.00

    Lambda 5 抗体 (A-1) FITC

    sc-398932 FITC
    200 µg/ml
    RMB2482.00

    Lambda 5 抗体 (A-1) PE

    sc-398932 PE
    200 µg/ml
    RMB2580.00

    Lambda 5 抗体 (A-1) Alexa Fluor® 488

    sc-398932 AF488
    200 µg/ml
    RMB2685.00

    Lambda 5 抗体 (A-1) Alexa Fluor® 546

    sc-398932 AF546
    200 µg/ml
    RMB2685.00

    Lambda 5 抗体 (A-1) Alexa Fluor® 594

    sc-398932 AF594
    200 µg/ml
    RMB2685.00

    Lambda 5 抗体 (A-1) Alexa Fluor® 647

    sc-398932 AF647
    200 µg/ml
    RMB2685.00

    Lambda 5 抗体 (A-1) Alexa Fluor® 680

    sc-398932 AF680
    200 µg/ml
    RMB2685.00

    Lambda 5 抗体 (A-1) Alexa Fluor® 790

    sc-398932 AF790
    200 µg/ml
    RMB2685.00

    I need to use an antibody to detect the protein in samples of porcine origin. Do you know if Lambda 5 (A-1): sc-398932 mouse monoclonal antibody will work?

    Asked by: jenniferc
    Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We are available by phone, e-mail or chat.
    Answered by: Technical Support
    Date published: 2017-03-01
    • y_2025, m_9, d_7, h_4CST
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    Rated 1 out of 5 by from IHC (P): very weak stainingThis antibody was tested in human spleen, tonsil and bone marrow paraffin sections (fixation 3.7% formol in tap water for 24 h, antigen retrievals investigated: without, protease XIV and autoclaving at pH 6.0 in citrate buffer)). Only weak staining was obtained in peri- and interfollicular areas of spleen and tonsil (antigen retrieval : autoclaving at pH 6.0 in citrate buffer). After tyramide enhancement the antibody stained small round mononuclear cells in bone marrow, in perifollicular areas of spleen and interfollicular areas of tonsils. This does not correspond to the known distribution of pre-B-lymphocytes, which should only occur in bone marrow.
    Date published: 2017-01-30
    Rated 5 out of 5 by from Very good Western blot data of Lambda 5 expressionVery good Western blot data of Lambda 5 expression in Jurkat, JM1 and CCRF-CEM whole cell lysates. -SCBT QC
    Date published: 2014-03-26
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    Lambda 5 抗体 (A-1) is rated 3.0 out of 5 by 2.
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