Soluble in ethanol, methanol, DMF or DMSO. Poor water solubility.
TEST CONDITIONS
HPLC Analysis and UV Spectrum: The HPLC chromatogram (254 nm) was generated on C18 reverse phase (EPS Platinum, Rocket format, Grace Discovery) under gradient conditions (0 to 100% ACN, 0.01% TFA, 10 min). The metabolite elutes as a single peak at 5.51 min displaying the expected UVmax at 216,238, 254, 276 and 342 nm with trace related analogue at 5.23 min (<1%) and no unrelated co-metabolites detected. Purity for batch >98% when analysed within the linear concentration/peak height response range. NOTE: the trace impurity is thought be loss of a saccharide subunit by facile methanolysis during work-up but is as yet unidentified.